Abstract：
:In the present study, human myometrial microvascular endothelial cells (HMMEC) were cocultured with human vascular smooth muscle cells (VSMC) labeled with fluorescent dyes to examine their morphological interaction using confocal laser fluorescent microscopy. HMMEC and VSMC labeled with fluorescent green and red dyes, respectively, attached to opposite sides of polyethyleneterephthalate membranes and remained viable for up to 96 h. In defined medium, 5%+/-3% of the VSMC cytoplasmic processes and 71%+/-17% of the HMMEC processes extended completely across the 13 microm thickness of the transmembrane. However, 41%+/-21% of the VSMC projections and 10%+/-3% of the HMMEC processes that traversed the membrane made contact with the opposing cell type. In cocultures incubated with angiopoietin-1 (Ang-1), although the number of VSMC or HMMEC projections was not significantly increased, the number of VSMC extending across the membrane and making contact with HMMEC was increased (P<0.05) to 88%+/-2%. The results of the current study demonstrate that coculture of fluorescent-labeled HMMEC and VSMC on a semipermeable transmembrane coupled with confocal laser fluorescent microscopy provides an in vitro experimental model to study morphological association of microvascular endothelial cells with mural cells. We propose that this system will greatly facilitate study of remodeling of the microvasculature in various organ systems.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Burch MG,Pepe GJ,Dobrian AD,Lattanzio FA Jr,Albrecht EDdoi
10.1016/j.mvr.2005.04.008keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
43-52issue
1-2eissn
0026-2862issn
1095-9319pii
S0026-2862(05)00052-Xjournal_volume
70pub_type
杂志文章abstract：:The cytoskeleton is vital to the function of virtually all cell types in the organism as it is required for cell division, cell motility, endo- or exocytosis and the maintenance of cell shape. Endothelial cells, lining the inner surface of the blood vessels, exploit cytoskeletal elements to ensure the integrity of cel...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2008.06.003
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:In this study we aimed to evaluate effects of liver resection on hepatic microcirculation. In addition we wanted to study if histological liver damage could be detected intra-operatively. PATIENTS AND METHODS:40 patients undergoing hepatic resection were included and grouped according to if they were operat...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...
journal_title：Microvascular research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.mvr.2010.01.002
更新日期：2010-03-01 00:00:00
abstract：:Spontaneous contractions and those elicited by electrical stimulation were studied in isolated segments of bovine mesenteric lymphatic vessels. The effects of three general anesthetics, pentobarbitone, halothane, and ether, on these spontaneous and evoked contractions were studied. Pentobarbitone and halothane inhibit...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90073-3
更新日期：1989-01-01 00:00:00
abstract：:The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.05.001
更新日期：2004-09-01 00:00:00
abstract：:Oxygen tension was recorded with double-barreled microelectrodes in the retina of anesthetized cats and monkeys. The oxygen tension (PO2) was often observed to undergo spontaneous irregular fluctuations with peak-to-peak amplitudes of about 3 to as much as 20 mm Hg. These fluctuations were observed only in the vascula...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90103-v
更新日期：1992-07-01 00:00:00
abstract：:It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90008-f
更新日期：1990-07-01 00:00:00
abstract：:Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.11.002
更新日期：2014-01-01 00:00:00
abstract：:During diabetes in rats, the choroid of the eye shows increased permeability to albumin, basement membrane thickening, and decreased anionic charge sites on the abluminal surfaces of the choriocapillary microvessels. In other microvascular beds, permeability differences are correlated with differences in luminal membr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90058-j
更新日期：1991-11-01 00:00:00
abstract：:Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104103
更新日期：2021-01-01 00:00:00
abstract：:We present a simple method for isolation and long-term cultivation of porcine and murine cerebral capillary endothelial cells (cEC). Two major points are made. First, that the "characteristic" morphology of the endothelial cells depends mainly on the presence of endothelial cell growth factors in the culture medium an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90034-4
更新日期：1989-03-01 00:00:00
abstract：:Living mesenteric capillaries with either an intact or disrupted glycocalyx were challenged with ramp change in shear stress (Deltatau). Animals (Rana pipiens) were divided randomly into two experimental groups, and two true capillaries (TC) per animal were investigated. The modified Landis technique was combined with...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.12.001
更新日期：2010-03-01 00:00:00
abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：:The contractile responses of cultured rat and calf endothelial cells (EC), vascular smooth muscle cells (VSMC), and fibroblasts (FB) to vasoactive mediators (thrombin, serotonin, bradykinin, and histamine), forskolin, and cytochalasin B were compared. Cells were grown on a pliable silicone membrane, and contraction wa...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90015-h
更新日期：1992-03-01 00:00:00
abstract：:To compute two parameters of capillary protein transport of reflection coefficient (sigma) and permeability--surface area product (PS), two major methods have been employed. The first, or crosspoint method, uses two sets of lymph flow (Jv) and lymph/plasma protein concentration ratios (R) and solves the irreversible t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90051-2
更新日期：1985-09-01 00:00:00
abstract：:Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate th...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.10.003
更新日期：2019-05-01 00:00:00
abstract：:The Raynaud's phenomenon often accompanies systemic rheumatic diseases and is also known as a vascular side effect of chemotherapy. Therapy of the Raynaud's phenomenon with nitrates or calcium-channel-blockers is rarely beneficial. In contrast, the PDE-V-inhibitor sildenafil seems to be effective in these patients. Fo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.03.001
更新日期：2005-05-01 00:00:00
abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2043
更新日期：1997-11-01 00:00:00
abstract：:The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1022
更新日期：1993-05-01 00:00:00
abstract：:The steroid hormone estradiol is suggested to play a protective role in intestinal injury during systemic inflammation (sepsis). Our aim was to determine the effects of specific estradiol receptor (ER-α and ER-ß) agonists on the intestinal microcirculation during experimental sepsis. Male and sham ovariectomized femal...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.10.002
更新日期：2013-01-01 00:00:00
abstract：:The anatomic arrangement, pressure distribution, and resting vascular tone of the feed arteries located upstream from the rat cremaster microcirculation were determined to characterize the sites of the vascular resistance in this macrovessel segment of the cremaster circulation. The cremaster microcirculation and its ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(87)90009-4
更新日期：1987-01-01 00:00:00
abstract：:Hypoxia-inducible factor-1 (HIF-1) orchestrates angiogenesis under hypoxic conditions mainly due to increased expression of such target genes as vascular endothelial growth factor (VEGF). Na+/H+exchanger-1 (NHE1), a potential HIF target gene product, plays a pivotal role in proliferation, survival, migration, adhesion...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.12.004
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:In the present study, we used a two-kidney-two-clip (2k2c) stroke-prone renovascular hypertension rat model (RHRSP) to investigate the protective effects of ligustrazine (TMP) on cerebral arteries and to examine PI3K/Akt pathway behavior under this protection. METHODS:The cerebral artery remodeling was induc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103938
更新日期：2020-03-01 00:00:00
abstract：:Neutrophil-derived hydrogen peroxide (H2O2) is believed to play an important role in inflammatory lung injury. We investigated the influence of pharmacological agents that increase intracellular c-AMP levels on endothelial and epithelial leakage in response to intravascular H2O2 challenge in buffer-perfused rabbit lun...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1033
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND:Angiogenin levels are increased in children and adolescent patients with type 1 diabetes, regardless of the extent of diabetic microangiopathy. However, little is known about the angiogenin concentrations in adults with type 1 diabetes. Thus we studied its level in middle aged subjects with the presence of d...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.08.005
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.10.008
更新日期：2014-03-01 00:00:00
abstract：:Local skin heating to 42°C causes rapid increases in cutaneous perfusion (initial peak), followed by a brief nadir and subsequent sustained elevation (plateau). Several studies have demonstrated that nitric oxide synthase (NOS) largely contributes to the plateau response during local heating. In this study, we tested ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.12.001
更新日期：2018-05-01 00:00:00
abstract：:The maturational changes in tissue water spaces and in the fractional exclusion of albumin from the interstitial gel were studied in the developing rabbit using multiple radioactive tracers. Total tissue water and extracellular water decrease with age in all organs studied. Intracellular water decreases in the heart a...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(86)90021-x
更新日期：1986-05-01 00:00:00
abstract：:A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.009
更新日期：2009-06-01 00:00:00
abstract：:Although vasodilation is conventionally held to be the predominant microvascular response to a wound, there has been no previous attempt to actually quantitate skin blood flow within and in the neighborhood of wounds. In particular, there has been no differentiation between sites with primarily nutritive (NUTR) blood ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2008
更新日期：1997-05-01 00:00:00
