Abstract:
:We present a simple method for isolation and long-term cultivation of porcine and murine cerebral capillary endothelial cells (cEC). Two major points are made. First, that the "characteristic" morphology of the endothelial cells depends mainly on the presence of endothelial cell growth factors in the culture medium and second, that the identification of the cells as endothelial cells requires a special lectin instead of criteria used for large vessel endothelial cells, such as factor VIII staining or LDL uptake. Pure cerebral capillaries were isolated by means of a series of centrifugation steps; endothelial cells were released by collagenase treatment and cultivated on plastic petri dishes, which proved to be better for cell attachment than collagen or gelatin coating. The microvascular cells were cultivated in either the presence or absence of growth factors. Medium 199 + 10% FCS produced mainly spindle-shaped cells, growing in the "hills and valleys" pattern, which, if not passaged for weeks, showed three dimensional tubular structures. Cells of the "cobblestone" phenotype were promoted in medium 199 + 10% FCS, enriched with endothelial cell growth supplement (ECGS) and heparin (referred to as complete medium). These cells retained their phenotype for months and could be passaged up to 35 times till now. If ECGS and heparin were omitted from these cultures, the cells became elongated and resembled smooth muscle cells. This effect was reversible when the cells were transferred to complete medium. With cEC, cloned by limiting dilution, we noticed this reversal phenomenon as well. We used several markers to characterize the microvascular cells and could show that the lectin of Bandeiraea simplicifolia is a highly reliable marker for endothelial cells and that the monoclonal antibody alpha-sm-1 (anti-smooth muscle cell actin) is excellent for determining smooth muscle cells.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Tontsch U,Bauer HCdoi
10.1016/0026-2862(89)90034-4subject
Has Abstractpub_date
1989-03-01 00:00:00pages
148-61issue
2eissn
0026-2862issn
1095-9319pii
0026-2862(89)90034-4journal_volume
37pub_type
杂志文章abstract::Polymorphonuclear leukocytes are thought to play a pivotal role in the generation of ischemia-reperfusion-induced mucosal injury; however, their behavior in the intestinal microvasculature following this injury has not been directly examined. In this study intravital microscopy was used to investigate the dynamics of ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1028
更新日期:1994-05-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the correlation between the expression of miR-210 in peripheral blood and the number of peripheral endothelial progenitor cells (EPCs) in patients with type 2 diabetes mellitus (T2DM). We also determined the effect of miR-210 on EPC proliferation, adhesion, migration, tube forma...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104032
更新日期:2020-09-01 00:00:00
abstract::Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of ne...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.12.001
更新日期:2015-03-01 00:00:00
abstract::Angiogenesis, or the growth of new blood vessels, is critical to both health and disease. Several genes are implicated in the regulation of angiogenesis, such as genes encoding growth factors, cytokines, and the extracellular matrix, as well as transcription factors. Among them, Hox genes exert master regulatory effec...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2013.02.009
更新日期:2013-05-01 00:00:00
abstract::This study was designed to determine if vascular dysfunction and enhanced norepinephrine sensitivity occurring in early experimental juvenile diabetes (S. M. Mueller, T. M. Mueller, and P. J. Ertel, 1982, Amer. J. Physiol. 243, H139-H144) persist, improve, or worsen in adulthood. Alloxan was administered to rats at 4 ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90030-x
更新日期:1984-07-01 00:00:00
abstract::The large secreted glycoprotein thrombospondin-1 is a potent inhibitor of neovascularization in vivo. In order to better understand its mechanism of action, we have determined the full range of deficits thrombospondin can impose on cultured capillary endothelial cells. Exogenously added thrombospondin-1 blocked the ab...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1997.2015
更新日期:1997-07-01 00:00:00
abstract::Changes in blood vessel diameter can be measured manually, but this is time-consuming and often impractical. For automatic measurement commercial solutions are available, but the proprietary algorithms and their potential shortcomings are not known to the user. We present an approach with a CCD camera for image acquis...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.04.004
更新日期:2010-09-01 00:00:00
abstract::The therapeutic efficacy of various genetically engineered macromolecules is determined by their delivery and distribution in tumors. We have recently developed mathematical models which describe the interstitial velocity, pressure, and concentration profiles of macromolecules over the length scale of a solid tumor (B...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90026-8
更新日期:1991-03-01 00:00:00
abstract::It has generally been assumed that movement of fluid between the pulmonary microvasculature and surrounding tissues is governed by a "Starling" balance of hydrostatic and protein osmotic forces similar to that which prevails in the extremities. However, both recent and older observations suggest that the lungs are mor...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2009.03.004
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:Ischemia-reperfusion (IR) injury impairs microcirculatory function by reducing nitric oxide (NO) bioavailability and increasing sympathetic tone. This study non-invasively examined the effects of acute upper limb IR injury on local thermal hyperemia (LTH) in glabrous and non-glabrous finger skin. MATERIALS ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.02.003
更新日期:2018-07-01 00:00:00
abstract::Current tissue engineering techniques have failed to provide an established microvasculature in skin substitutes, a requisite for the maintenance of graft viability and rapid revascularization subsequent to graft transplantation in vivo. To improve outcomes for both conventional skin grafts and skin substitutes, the e...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2007.04.012
更新日期:2008-01-01 00:00:00
abstract::Metabolic syndrome (MetS) is a combination of clinical disorders that together increase the risk for cardiovascular disease and diabetes. SHRSP.Z-Lepr(fa)/IzmDmcr (SHRSP.ZF) rats with MetS show impaired nitric oxide-mediated relaxation in coronary and mesenteric arteries, and angiotensin II receptor type 1 blockers pr...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.04.001
更新日期:2013-07-01 00:00:00
abstract::IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.02.007
更新日期:2009-06-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.10.004
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2236
更新日期:2000-07-01 00:00:00
abstract::Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.07.006
更新日期:2009-12-01 00:00:00
abstract::Blood flow studies in the brain, heart, and other organs suggest that there could be interaction between nitric oxide (NO) and adenosine. This possibility was investigated in the optic nerve head (ONH) during photic stimulation of the dark-adapted cat eye. Functional activation of ONH blood flow was measured by laser ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2002.2432
更新日期:2002-09-01 00:00:00
abstract:OBJECTIVES:Calcium channel inhibitors have beneficial impact on microcirculation, but beta-blocker effect is controversial. Clinicians still do not agree on beta-blocker combination with other treatments in the management of impaired microcirculation. The aim of the present study was to describe the effects of beta-blo...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.04.004
更新日期:2011-07-01 00:00:00
abstract::A microfluidic device was developed for quantifying the migratory and deformability capabilities of a single tumor cell using direct imaging. It was fabricated using photolithography and is made of polydimethysiloxane. Chemotaxis approach was used for directing cell movement, using 10 microm microgaps to restrict the ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2006.06.003
更新日期:2006-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00005-0
更新日期:2003-05-01 00:00:00
abstract::Reduced lung capillary expression of angiotensin I-converting enzyme (ACE), a key enzyme in cardiovascular pathophysiology, and of caveolin-1, an important regulator of endothelial cell signalling, has been demonstrated in various models of pulmonary arterial hypertension (PAH). We addressed the relationship between P...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.04.008
更新日期:2010-09-01 00:00:00
abstract::The relative magnitude of vascular reactivity to inhaled gas stimuli in the major retinal arterioles has not been systematically investigated. The purpose of this study was to compare the magnitude of retinal vascular reactivity in response to inhaled gas provocation at equivalent measurement sites in the superior-, a...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2011.11.002
更新日期:2012-03-01 00:00:00
abstract::Microvascular blood flow is commonly calculated from a "centerline" velocity derived from the dual-slit method. This is done by relating the computed centerline velocity to an average velocity through an empirically derived conversion factor. To determine the physical basis of the conversion factor, we utilized a Four...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(89)90046-0
更新日期:1989-05-01 00:00:00
abstract::We examined the influence of local cooling (-15 degrees C, 60 sec) on blood flow velocity in nailfold capillaries and on laser Doppler flux (LDF) at the fingertip in 12 patients with primary Raynaud's phenomenon (PRP) and 13 patients with secondary Raynaud's (SRP) in comparison with 10 health controls. Skin temperatur...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1993.1060
更新日期:1993-11-01 00:00:00
abstract::We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...
journal_title:Microvascular research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.mvr.2010.01.002
更新日期:2010-03-01 00:00:00
abstract::In this study cerebral capillary bed structure and the effects of chronic hypertension on these systems have been assessed in 6- to 7-month-old spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Capillary diameter (D), profile frequency (Na), volume fraction (Vv), and surface area (Sv) were quantitated by l...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(90)90032-m
更新日期:1990-11-01 00:00:00
abstract::Previously reported cell-flow microchannels in a single-crystal silicon substrate (Microvasc. Res. 44, 226-240, 1992) have been modified, and flow behavior of blood cells is described using flow rate-time curves and video pictures. The principal structure (2600 identically sized channels in parallel) was retained to g...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1008
更新日期:1994-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.10.008
更新日期:2015-01-01 00:00:00
abstract::In the present study, human myometrial microvascular endothelial cells (HMMEC) were cocultured with human vascular smooth muscle cells (VSMC) labeled with fluorescent dyes to examine their morphological interaction using confocal laser fluorescent microscopy. HMMEC and VSMC labeled with fluorescent green and red dyes,...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2005.04.008
更新日期:2005-07-01 00:00:00
abstract::The effect of hemodilution on oxygen delivery to the tissue was investigated analytically by taking into consideration the oxygen loss that occurs along the arterial microvasculature due to diffusion into the tissue and shunting to parallel running venules. The theoretical findings were related to experimental data on...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(87)90062-8
更新日期:1987-11-01 00:00:00