Abstract:
:Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of neovessels. However, contradictory results are published concerning the vasculogenic potential of this cell type. We could previously show that implanted human endothelial vein cells (HUVECs) gave rise to the formation of a complex functional human neovasculature in a heterotopic (subcutaneous) as well as in an orthotopic (calvarial defect) model of severe combined immunodeficiency (SCID) mice, where vessel formation could even be increased by coimplanting mesenchymal stem cells (MSCs) functioning as perivascular cells. In this study, we investigated whether coimplantation of MSCs which have been predifferentiated in vitro into SMCs (SMC-MSCs) may enable pbEPCs to form blood vessels upon implantation and, if this would be the case, whether the resulting enhanced vascularization may support bone regeneration. For this purpose, pbEPCs and SMC-MSCs were mono- or cocultured in collagen matrices and seeded into scaffolds consisting of decalcified processed bovine cancellous bone (PBCB, Tutobone). Neovascularization and osteogenesis were evaluated using a calvarial bone defect-model in SCID mice. Our experiments could show that the missing vasculogenic potential of pbEPCs is not rescued by coimplantation of SMCs derived from MSCs predifferentiated along the vascular smooth muscle lineage. However, implantation of both cell types alone, or in combination induced an angiogenic response, which correlated in a positive manner with bone formation within the implants.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Goerke SM,Obermeyer J,Plaha J,Stark GB,Finkenzeller Gdoi
10.1016/j.mvr.2014.12.001subject
Has Abstractpub_date
2015-03-01 00:00:00pages
40-7eissn
0026-2862issn
1095-9319pii
S0026-2862(14)00188-5journal_volume
98pub_type
杂志文章abstract::The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.05.008
更新日期:2014-07-01 00:00:00
abstract::Pericytes are perivascular support cells, the origin of which in tumor tissue is not clear. Recently, we identified a Tie1(+) precursor cell that differentiates into vascular smooth muscle, in a Notch-dependent manner. To understand the involvement of Notch in the ontogeny of tumor pericytes we used a novel flow immun...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.05.004
更新日期:2015-09-01 00:00:00
abstract::This study was undertaken to determine if coronary vascular permeability (CVP) increases and if myocardial edema develops in the canine heart after local exposure to histamine. Histamine (50 or 15 micrograms/min) or compound 48/80 (0.1-0.2 mg/kg) was infused into the left anterior descending coronary artery (LAD) of o...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90034-9
更新日期:1991-05-01 00:00:00
abstract::Metabolic syndrome (MetS) is a combination of clinical disorders that together increase the risk for cardiovascular disease and diabetes. SHRSP.Z-Lepr(fa)/IzmDmcr (SHRSP.ZF) rats with MetS show impaired nitric oxide-mediated relaxation in coronary and mesenteric arteries, and angiotensin II receptor type 1 blockers pr...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.04.001
更新日期:2013-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.07.006
更新日期:2012-11-01 00:00:00
abstract::Microalbuminuria is a primary manifestation of diabetic nephropathy. Endothelial cell-selective adhesion molecule (ESAM) is a new member of the immunoglobulin superfamily which is selectively expressed by vascular endothelial cells. Although ESAM mediates homophilic interaction between endothelial cells, the role of E...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.01.002
更新日期:2009-05-01 00:00:00
abstract::We examined the influence of local cooling (-15 degrees C, 60 sec) on blood flow velocity in nailfold capillaries and on laser Doppler flux (LDF) at the fingertip in 12 patients with primary Raynaud's phenomenon (PRP) and 13 patients with secondary Raynaud's (SRP) in comparison with 10 health controls. Skin temperatur...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1993.1060
更新日期:1993-11-01 00:00:00
abstract::The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90060-8
更新日期:1984-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.01.006
更新日期:2012-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2363
更新日期:2002-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(89)90020-4
更新日期:1989-07-01 00:00:00
abstract::Pericytes are known to communicate with endothelial cells by direct contact and by releasing cytokines such as TGF-beta. There is also strong evidence that pericytes act as regulators of endothelial cell proliferation and differentiation. We have investigated the effect of pericyte-conditioned medium (PCM) on prolifer...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2208
更新日期:2000-01-01 00:00:00
abstract::We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2356
更新日期:2001-11-01 00:00:00
abstract::Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.03.001
更新日期:2010-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2177
更新日期:1999-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(92)90089-8
更新日期:1992-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.11.003
更新日期:2019-03-01 00:00:00
abstract::A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90071-i
更新日期:1991-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2017.09.003
更新日期:2018-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.07.006
更新日期:2008-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2007.01.002
更新日期:2007-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00005-0
更新日期:2003-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.09.008
更新日期:2009-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.008
更新日期:2018-11-01 00:00:00
abstract::This study determined the contribution of cytochrome P450 (CP450) 4A enzyme metabolites of arachidonic acid in mediating the constriction of isolated rat skeletal muscle resistance arteries in response to elevated PO2. Gracilis arteries (GA) were viewed via television microscopy and constrictor responses to elevated P...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2341
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND:Sedentary behaviour is linked to increased cardiovascular disease risk in Rheumatoid Arthritis (RA), but the biological processes underlying this relationship are not understood. OBJECTIVES:To investigate the cross-sectional associations of habitual sedentary behaviour, with endothelial function in RA. MET...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.01.005
更新日期:2018-05-01 00:00:00
abstract::Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104103
更新日期:2021-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(87)90009-4
更新日期:1987-01-01 00:00:00
abstract::The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90040-8
更新日期:1985-07-01 00:00:00
abstract::This study aims to assess the relationship between spontaneous oscillations in changes in cerebral tissue oxyhemoglobin concentrations (Delta [HbO2]) and arterial blood pressure (ABP) signals in healthy elderly subjects during the resting state using wavelet coherence analysis. Continuous recordings of near-infrared s...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.008
更新日期:2014-05-01 00:00:00