Abstract:
OBJECTIVE:This study aims to investigate the correlation between the expression of miR-210 in peripheral blood and the number of peripheral endothelial progenitor cells (EPCs) in patients with type 2 diabetes mellitus (T2DM). We also determined the effect of miR-210 on EPC proliferation, adhesion, migration, tube formation, and apoptosis. METHODS:A total of 32 patients with newly diagnosed T2DM (T2DM group) and 32 control subjects with normal glucose tolerance (NC group) were included. Peripheral blood samples were collected from each subject. The miR-210 level was determined by quantitative real-time polymerase chain reaction (qRT-PCR), and the number of positive EPCs indicated by CD34, CD133, and KDR expressions was detected by flow cytometry. After isolation, culture, and identification by fluorescent staining, EPCs were divided into four groups: NC group, untransfected type 2 diabetic group, miR-210 inhibitor NC group, and miR-210 inhibitor group. The expression of miR-120 in each group was detected by qRT-PCR, and the changes in the proliferation, adhesion, migration, tube formation, and apoptosis of EPCs after transfection with a miR-210 inhibitor were observed. RESULTS:The expression level of miR-210 in the T2DM group (5.83 ± 1.26) was significantly higher than that in the NC group (1.18 ± 0.54) (t = 17.26, P < 0.001). The number of EPCs was significantly lower in the T2DM group (39.3 ± 12.6)/106 cells than that in the NC group (76.2 ± 10.7)/106 cells (t = 10.49, P < 0.001). Spearman's correlation analysis showed that the expression of miR-210 in the peripheral blood of patients with T2DM was negatively correlated with the number of EPCs (r = -0.558, P = 0.001). Multiple linear stepwise regression analysis showed that the peripheral blood level of miR-210 was an independent correlation factor that affected the number of EPCs (P < 0.001). After transfection with the miR-210 inhibitor, the proliferation, adhesion, tube formation, and migration levels of EPCs in miR-210 inhibitor group were higher than those in untransfected type 2 diabetic group and miR-210 inhibitor NC group, whereas the apoptosis rate was lower than that in these groups, and these results were statistically significant (P < 0.05). CONCLUSION:The increased expression of miR-210 in patients with T2DM may be related to the decreased number and function of EPCs in peripheral blood.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Li X,Jia Z,Zhao X,Xu M,Chen Mdoi
10.1016/j.mvr.2020.104032subject
Has Abstractpub_date
2020-09-01 00:00:00pages
104032eissn
0026-2862issn
1095-9319pii
S0026-2862(20)30092-3journal_volume
131pub_type
杂志文章abstract::7-Ketocholesterol (7kCh) is a major oxysterol found associated with vascular diseases. Human microvascular endothelial cells (HMVECs) were cultured with different concentrations of 7kCh with and without inhibitors. Cell viabilities and caspase activities were assessed. 7kCh caused loss of cell viability in a dose-depe...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2007.10.003
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVE:Studies on the acute effects of coffee on the microcirculation have shown contradicting results. This study aimed to investigate if intake of caffeine-containing coffee changes blood flow and microvascular reactivity in the skin. METHODS:We measured acute changes in cutaneous vascular conductance (CVC) in th...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2017.06.006
更新日期:2017-11-01 00:00:00
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journal_title:Microvascular research
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doi:10.1006/mvre.1994.1063
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abstract::An increased recognition of the role of endothelial cells in disease and the development of methods for endothelial cell culture has led to an upsurge in in vitro studies of endothelial cell function. However, the cells most often used for these studies do not reflect the in vivo heterogeneity of endothelial cells. To...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1997.2045
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abstract::The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, howe...
journal_title:Microvascular research
pub_type: 杂志文章
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journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2010.09.005
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journal_title:Microvascular research
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doi:10.1006/mvre.1996.0029
更新日期:1996-05-01 00:00:00
abstract::Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.06.011
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2003.09.003
更新日期:2004-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0026-2862(02)00010-9
更新日期:2003-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.103938
更新日期:2020-03-01 00:00:00
abstract::There is evidence that vascular beds distal to the ophthalmic artery (OA) show vasoconstriction in response to a step decrease in systemic blood pressure (BP). The mediators of this response are mostly unidentified. The aim of the current study was to test the hypothesis that α2-adrenoreceptors may contribute to the r...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2010.10.001
更新日期:2011-01-01 00:00:00
abstract::The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(02)00035-3
更新日期:2003-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2151
更新日期:1999-07-01 00:00:00
abstract::Neutrophil-derived hydrogen peroxide (H2O2) is believed to play an important role in inflammatory lung injury. We investigated the influence of pharmacological agents that increase intracellular c-AMP levels on endothelial and epithelial leakage in response to intravascular H2O2 challenge in buffer-perfused rabbit lun...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1033
更新日期:1995-07-01 00:00:00
abstract::This article describes the interaction between solid particles and blood propagating through a capillary. A slip condition is considered on the walls of the capillary. The rheological features of the blood are discussed by considering as a two-phase Newtonian fluid model, i.e., the suspension of cells in plasma. A per...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.04.004
更新日期:2018-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2005.04.007
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVE:Hypertension and hypercholesterolemia elicit inflammatory and thrombogenic responses in the microvasculature. However, little is known about whether and how risk factor combinations alter microvascular function. We examined how the actions of HTN+HCh on the microvasculature differ from the responses elicited ...
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pub_type: 杂志文章
doi:10.1016/j.mvr.2016.01.002
更新日期:2016-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1996.0016
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doi:10.1006/mvre.1999.2224
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journal_title:Microvascular research
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doi:10.1006/mvre.1993.1022
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journal_title:Microvascular research
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doi:10.1016/j.mvr.2004.08.001
更新日期:2004-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90035-0
更新日期:1988-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1028
更新日期:1994-05-01 00:00:00
abstract::The contractile responses of cultured rat and calf endothelial cells (EC), vascular smooth muscle cells (VSMC), and fibroblasts (FB) to vasoactive mediators (thrombin, serotonin, bradykinin, and histamine), forskolin, and cytochalasin B were compared. Cells were grown on a pliable silicone membrane, and contraction wa...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(92)90015-h
更新日期:1992-03-01 00:00:00
abstract::Fluid homeostasis is required for life. Processes involved in fluid balance are strongly related to exchanges at the microvascular level. Computational models have been presented in the literature to analyze the microvascular-interstitial interactions. As far as we know, none of those models consider a physiological d...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.11.003
更新日期:2019-03-01 00:00:00
abstract::Endotoxemia, a major feature of sepsis, is a common cause of acute lung injury and initiates rapid accumulation of leukocytes in the lung vasculature. Endothelial mechanisms that underlie this accumulation remain unclear, as current experimental models of endotoxemia are less suitable for targeted activation of the en...
journal_title:Microvascular research
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doi:10.1016/j.mvr.2012.01.006
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journal_title:Microvascular research
pub_type: 杂志文章
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.06.008
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