MiR-150 promotes angiogensis and proliferation of endothelial progenitor cells in deep venous thrombosis by targeting SRCIN1.

Abstract:

:Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate the effect of miR-150 on EPCs function ex vivo and thrombus resolution in vivo. We determined miR-150 expression in EPCs isolated from DVT patients and control subjects by RT-PCR. Potential target of miR-150 was confirmed by bioinformatics analysis and luciferase reporter respectively. The angiogenesis and proliferation were tested by MTT and tube formation assay. A murine model of venous thrombosis was developed as in vivo model. Finally, the effect of miR-150 on EPCs with inferior venous thrombosis were evaluated in vivo. Our data showed that miR-150 was downregulated in EPCs from DVT patients. By using miR-150 agomir and antagomir, we found that miR-150 promoted angiogenesis and proliferation of EPCs. Bioinformatics analysis revealed SRCIN1 as a target of miR-150 and SRCIN1 knockdown inhibited function of EPCs. Forced expression of miR-150 contributed thrombus resolution in a murine model of venous thrombosis. In general, miR-150 was downregulated in EPCs from DVT. Upregulation of miR-150 promoted angiogenesis and proliferation of EPCs by targeting SRCIN1 in vitro and thrombus resolution in vivo.

journal_name

Microvasc Res

journal_title

Microvascular research

authors

Wang W,Zhu X,Du X,Xu A,Yuan X,Zhan Y,Liu M,Wang S

doi

10.1016/j.mvr.2018.10.003

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

35-41

eissn

0026-2862

issn

1095-9319

pii

S0026-2862(18)30131-6

journal_volume

123

pub_type

杂志文章
  • Characteristics of beat-to-beat photoplethysmography waveform indexes in subjects with metabolic syndrome.

    abstract:UNLABELLED:Metabolic syndrome (MetS) increases the risk of the subsequent development of cardiovascular disease. This study aimed to determine if the harmonic indexes of finger photoplethysmography (PPG) waveforms can be used to discriminate different arterial pulse transmission conditions between MetS and healthy subj...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2016.04.001

    authors: Chang YW,Hsiu H,Yang SH,Fang WH,Tsai HC

    更新日期:2016-07-01 00:00:00

  • Influence of local finger cooling on laser Doppler flux and nailfold capillary blood flow velocity in normal subjects and in patients with Raynaud's phenomenon.

    abstract::We examined the influence of local cooling (-15 degrees C, 60 sec) on blood flow velocity in nailfold capillaries and on laser Doppler flux (LDF) at the fingertip in 12 patients with primary Raynaud's phenomenon (PRP) and 13 patients with secondary Raynaud's (SRP) in comparison with 10 health controls. Skin temperatur...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1993.1060

    authors: Lütolf O,Chen D,Zehnder T,Mahler F

    更新日期:1993-11-01 00:00:00

  • Neuropeptide Y not involved in cutaneous vascular control in young human females taking oral contraceptive hormones.

    abstract::We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2017.04.003

    authors: Hodges GJ,Martin ZT,Del Pozzi AT

    更新日期:2017-09-01 00:00:00

  • Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo.

    abstract::Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2010.03.001

    authors: Critser PJ,Kreger ST,Voytik-Harbin SL,Yoder MC

    更新日期:2010-07-01 00:00:00

  • Stereomorphological arrangement of capillaries in skeletal muscle in normal and stenosed young and adult rats.

    abstract::The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(84)90060-8

    authors: Brotzu G,Carta M,D'Alia G,Diaz G,Montisci R,Savona G

    更新日期:1984-05-01 00:00:00

  • Chebulagic acid and Chebulinic acid inhibit TGF-β1 induced fibrotic changes in the chorio-retinal endothelial cells by inhibiting ERK phosphorylation.

    abstract:PURPOSE:Diabetic retinopathy (DR) is characterized by pro-inflammatory, pro-angiogenic and pro-fibrotic environment during the various stages of the disease progression. Basement membrane changes in the retina and formation of fibrovascular membrane are characteristically seen in DR. In the present study the effect of ...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2018.09.001

    authors: Shanmuganathan S,Angayarkanni N

    更新日期:2019-01-01 00:00:00

  • Partial chemical characterization of the anionic sites in the basal lamina of fenestrated capillaries.

    abstract::The distribution of anionic sites in the basal laminae of the blood capillaries of the murine pancreas was studied in specimens fixed in ruthenium red (RR)-glutaraldehyde mixtures. The sites appeared as discrete, small (6 to 18 nm) particles distributed throughout the three laminae but concentrated primarily in the la...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(84)90006-2

    authors: Simionescu M,Simionescu N,Palade GE

    更新日期:1984-11-01 00:00:00

  • Hemorrhagic shock and nitric oxide release from erythrocytic nitric oxide synthase: a quantitative analysis.

    abstract::A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2009.02.009

    authors: Chen K,Pittman RN,Popel AS

    更新日期:2009-06-01 00:00:00

  • Adhesive interaction of functionalized particles and endothelium in idealized microvascular networks.

    abstract:OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2013.03.007

    authors: Lamberti G,Tang Y,Prabhakarpandian B,Wang Y,Pant K,Kiani MF,Wang B

    更新日期:2013-09-01 00:00:00

  • Impaired function of cerebral parenchymal arterioles in experimental preeclampsia.

    abstract::Preeclampsia (PE), a dangerous hypertensive complication of pregnancy, is associated with widespread maternal vascular dysfunction. However, the effect of PE on the cerebral vasculature that can lead to stroke and cognitive decline is not well understood. We hypothesized that function of cortical parenchymal arteriole...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2018.04.007

    authors: Johnson AC,Cipolla MJ

    更新日期:2018-09-01 00:00:00

  • Griffonia simplicifolia I: fluorescent tracer for microcirculatory vessels in nonperfused thin muscles and sectioned muscle.

    abstract::Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly when fluorescent visu...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(88)90022-2

    authors: Hansen-Smith FM,Watson L,Lu DY,Goldstein I

    更新日期:1988-11-01 00:00:00

  • Effects of ischemia on capillary density and flow velocity in nailfolds of human toes.

    abstract::The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(85)90040-8

    authors: Richardson D,Schwartz R,Hyde G

    更新日期:1985-07-01 00:00:00

  • Regulatory crosstalk between Hox genes and miRNAs during angiogenesis.

    abstract::Angiogenesis, or the growth of new blood vessels, is critical to both health and disease. Several genes are implicated in the regulation of angiogenesis, such as genes encoding growth factors, cytokines, and the extracellular matrix, as well as transcription factors. Among them, Hox genes exert master regulatory effec...

    journal_title:Microvascular research

    pub_type: 杂志文章,评审

    doi:10.1016/j.mvr.2013.02.009

    authors: Mahdipour E

    更新日期:2013-05-01 00:00:00

  • Validation of near-infrared laser speckle imaging for assessing microvascular (re)perfusion.

    abstract::The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2010.01.004

    authors: Bezemer R,Klijn E,Khalilzada M,Lima A,Heger M,van Bommel J,Ince C

    更新日期:2010-03-01 00:00:00

  • Modulation of endothelial cell network formation in vitro by molecular signaling of head and neck squamous cell carcinoma (HNSCC) exposed to cetuximab.

    abstract::Overexpression of EGFR plays a key-role in head and neck squamous cell carcinoma (HNSCC) and justifies the extensive use of cetuximab, a monoclonal anti-EGFR antibody, as well as EGFR-tyrosine kinase inhibitors (EGFR-TKI), which have been reported to inhibit tumor cell growth and the secretion of pro-angiogenic factor...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2011.07.008

    authors: Jouan-Hureaux V,Boura C,Merlin JL,Faivre B

    更新日期:2012-03-01 00:00:00

  • Chronic elevations in salt intake and reduced renal mass hypertension compromise mechanisms of arteriolar dilation.

    abstract::Alterations in the reactivity of arterioles to vasodilator agonists were assessed in the skeletal muscle microcirculation of age-matched, normotensive male Sprague-Dawley rats fed either a high salt (4% NaCl, HS) or a low salt (0.4% NaCl, LS) diet and in reduced renal mass hypertensive rats on a high salt diet (HSRRM)...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1998.2105

    authors: Frisbee JC,Lombard JH

    更新日期:1998-11-01 00:00:00

  • Microvascular hemodynamics in experimental arthritis: disparity between the distribution of microspheres and plasma flow in bone.

    abstract::The microcirculation in normal and arthritic juxtaarticular bone was studied in 16 young dogs with carragheenan-induced arthritis of one knee. The regional blood flow was determined by the tissue uptake of intracardially injected 15-microns 141Ce-labeled microspheres, and the microvascular plasma volume was determined...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(90)90020-r

    authors: Hansen ES,Søballe K,Kjølseth D,Henriksen TB,He SZ

    更新日期:1990-09-01 00:00:00

  • Effects of pulmonary ischemia-reperfusion on platelet adhesion in subpleural arterioles in rabbits.

    abstract::Reperfusion of the ischemic lung is associated with increased pulmonary vascular resistance and reduced alveolar perfusion in conjunction with an inflammatory response. To determine the contribution of platelet-endothelial interactions, we examined effects of pulmonary ischemia-reperfusion (IR) on platelet adhesion an...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2003.09.003

    authors: Roberts AM,Ovechkin AV,Mowbray JG,Robinson TW,Lominadze D

    更新日期:2004-01-01 00:00:00

  • Transvascular albumin and IgG flux in skin and skeletal muscle following plasmapheresis.

    abstract::The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(92)90089-8

    authors: Wallace JR,Powers MR,Bell DR

    更新日期:1992-11-01 00:00:00

  • Inhibition of capillary growth in chronically stimulated rat muscles by N(G)-nitro-l-arginine, nitric oxide synthase inhibitor.

    abstract::Shear stress causes release of nitric oxide (NO) from microvascular endothelial cells in vivo and stimulates their growth in vitro. After chronic electrical stimulation of lower hind limb skeletal muscles in the rat, measurements of capillary diameters and red blood cell velocity indicated that shear stress is increas...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1999.2193

    authors: Hudlická O,Brown MD,Silgram H

    更新日期:2000-01-01 00:00:00

  • Mechanism of macromolecule concentration in collecting lymphatics in rat mesentery.

    abstract::The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1997.2043

    authors: Takahashi T,Shibata M,Kamiya A

    更新日期:1997-11-01 00:00:00

  • A new model for chronic in vivo analysis of the periosteal microcirculation.

    abstract:OBJECTIVE:Microvascular perfusion is indispensable for the growth and remodulation of membrane bone. Trauma, inflammation and surgical interventions may alter periosteal perfusion. However, there is not much known about periosteal perfusion in membrane bones. Therefore, the aim of this study was to establish a new chro...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2008.09.008

    authors: Stuehmer C,Schumann P,Bormann KH,Laschke MW,Menger MD,Gellrich NC,Rücker M

    更新日期:2009-03-01 00:00:00

  • Regional differences in spontaneously occurring angiogenesis in the adult rat mesentery.

    abstract::Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1994.1029

    authors: Hansen-Smith FM,Joswiak GR,Baustert JL

    更新日期:1994-05-01 00:00:00

  • Effects of nonsteroidal anti-inflammatory drugs on microvascular dynamics.

    abstract::Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1006/mvre.1993.1016

    authors: Slater C,House SD

    更新日期:1993-03-01 00:00:00

  • Emerging from the PAC: studying zebrafish lymphatic development.

    abstract::Recently the zebrafish has emerged as a promising vertebrate model of lymphatic vasculature development. The establishment of numerous transgenic lines that label the lymphatic endothelium in the zebrafish has allowed the fine examination of the developmental timing and the anatomy of their lymphatic vasculature. Alth...

    journal_title:Microvascular research

    pub_type: 杂志文章,评审

    doi:10.1016/j.mvr.2014.06.001

    authors: Mulligan TS,Weinstein BM

    更新日期:2014-11-01 00:00:00

  • Skin microcirculatory effect of exogenous calcitonin gene-related peptide (CGRP) evaluated by laser Doppler flowmetry coupled with iontophoresis in healthy subjects.

    abstract::The aim of our study was to evaluate the feasibility of laser Doppler flowmetry (LDF) coupled with iontophoresis in exploring the skin vasodilator activity of exogenous calcitonin gene-related peptide (CGRP) in healthy subjects and to investigate the mechanisms involved in the skin vasodilator activity of this peptide...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2006.10.006

    authors: Rossi M,Carpi A,Di Maria C,Galetta F,Santoro G

    更新日期:2007-03-01 00:00:00

  • The use of quantum dots for analysis of chick CAM vasculature.

    abstract::Quantum dots (QDs) are fluorescent semiconductor nanocrystals that possess a number of superior fluorescent properties compared to more established organic dyes and fluorescent proteins. As a result, QDs are being studied for use in a wide range of biological applications. We have examined QDs for one such application...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2006.09.003

    authors: Smith JD,Fisher GW,Waggoner AS,Campbell PG

    更新日期:2007-03-01 00:00:00

  • Effect of dispersion of vessel diameters and lengths in stochastic networks. II. Modeling of microvascular hematocrit distribution.

    abstract::A microvascular network model described in the preceding paper (B. Dawant, M. Levin, and A. S. Popel, 1986, Microvasc. Res. 31, 203-222) featuring random distribution of vessel diameters and lengths, is extended to calculate the distributions of red blood cell flux and discharge hematocrit throughout the network. A re...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/0026-2862(86)90036-1

    authors: Levin M,Dawant B,Popel AS

    更新日期:1986-03-01 00:00:00

  • Expression and regulation of tumor necrosis factor-α-induced protein-8-like 2 is associated with acute lung injury induced by myocardial ischemia reperfusion in diabetic rats.

    abstract:AIMS:The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. METHODS:Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR); diabetes+sham (D +...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2020.104009

    authors: Kong Q,Yuan M,Ming T,Fang Q,Wu X,Song X

    更新日期:2020-07-01 00:00:00

  • Characterization of ultrasound-induced pulmonary capillary hemorrhage in rats.

    abstract::Routine pulmonary ultrasound for diagnosis of disease or injury relies on interpretation of image features, such as comet-tail artifacts, which can also be indicative of the poorly understood phenomenon of ultrasound-induced pulmonary capillary hemorrhage (PCH). Evans blue extraction and bronchoalveolar lavage (BAL) w...

    journal_title:Microvascular research

    pub_type: 杂志文章

    doi:10.1016/j.mvr.2014.02.006

    authors: Miller DL,Suresh MV,Dou C,Yu B,Raghavendran K

    更新日期:2014-05-01 00:00:00