Abstract:
:Neutrophil-derived hydrogen peroxide (H2O2) is believed to play an important role in inflammatory lung injury. We investigated the influence of pharmacological agents that increase intracellular c-AMP levels on endothelial and epithelial leakage in response to intravascular H2O2 challenge in buffer-perfused rabbit lungs. Endothelial permeability was assessed by determination of the capillary filtration coefficient (Kfc) and lung weight gain. Measurement of the clearance rate of inhaled aerosolized technetium-99m-labeled diethylenetriamine pentaacetic acid ([99mTc]DTPA) from the lungs into the perfusion fluid was used as an index of alveolar epithelial permeability. Experiments were performed in the presence of acetylsalicylic acid to suppress H2O2-induced lung prostanoid generation and concomitant vasoconstriction. Under these conditions, H2O2 admixture to the perfusate (250 microM) caused a greater than eight-fold increase in Kfc values, resulting in > 30 g lung weight gain within 30 min in the absence of any significant vasopressor response. Pretreatment with the adenylate cyclase activators prostaglandin E1 (0.1 microM) and forskolin (0.1 microM), the dual phosphodiesterase type III/IV inhibitor zardaverine (10 microM) as well as combinations of these drugs all caused a nearly complete suppression of this early Kfc increase; and severe edema formation (> 30 g) was retarded to approximately 50-55 min. In addition to the microvascular leakage response, H2O2 caused a four- to five-fold increase in the [99mTc]DTPA clearance rate, starting within 15 min and culminating after approximately 35 min. Adenylate cyclase activation reduced this epithelial leakage response by approximately 30%, whereas zardaverine exerted no significant effect. We conclude that both microvascular endothelial and alveolar epithelial barrier function are severely compromised by intravascular H2O2 challenge in intact lungs. Pharmacological approaches to increase c-AMP levels, including both adenylate cyclase activation and phosphodiesterase inhibition, partially block the endothelial response and, to a lesser extent, the epithelial response.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Seeger W,Hansen T,Rössig R,Schmehl T,Schütte H,Krämer HJ,Walmrath D,Weissmann N,Grimminger F,Suttorp Ndoi
10.1006/mvre.1995.1033subject
Has Abstractpub_date
1995-07-01 00:00:00pages
1-17issue
1eissn
0026-2862issn
1095-9319pii
S0026-2862(85)71033-3journal_volume
50pub_type
杂志文章abstract:OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.03.007
更新日期:2013-09-01 00:00:00
abstract::Alzheimer's disease (AD) is a chronic neuro-degenerative disease that adversely affect many people on a global scale. Despite different diagnostic and therapeutic treatment, there is no cure for AD. The brain is one of the most complex organ and researchers are still trying to understand so as to find a cure. OBJECTI...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.103928
更新日期:2020-03-01 00:00:00
abstract::Circulation of blood depends, in part, on the ability of red blood cells (RBCs) to aggregate, disaggregate, and deform. The primary intrinsic disaggregating force of RBCs is derived from their electronegativity, which is largely determined by sialylated glycoproteins on the plasma membrane. Given supraphysiological sh...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.008
更新日期:2018-11-01 00:00:00
abstract::The purpose of the present study was to investigate the nervous control of gingival blood flow in cats. Gingival blood flow was measured by laser Doppler flowmeter in 75 cats during electrical stimulation and cutting or ligation of the inferior alveolar nerve and cervical sympathetic nerve without sympathectomy or pre...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(90)90061-u
更新日期:1990-01-01 00:00:00
abstract::An ex vivo perfused solid tumor preparation provides control over the physiological, biochemical, and pharmacological composition of the arterial input and easy access to the venous output. This is advantageous for studies of transport and metabolism in solid tumors. Here we present a tissue-isolated tumor preparation...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1063
更新日期:1994-11-01 00:00:00
abstract::A microvascular network model described in the preceding paper (B. Dawant, M. Levin, and A. S. Popel, 1986, Microvasc. Res. 31, 203-222) featuring random distribution of vessel diameters and lengths, is extended to calculate the distributions of red blood cell flux and discharge hematocrit throughout the network. A re...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(86)90036-1
更新日期:1986-03-01 00:00:00
abstract::Post occlusive skin reactive hyperemia (PORH) is a tool used to assess microcirculation. Endothelial nitric oxide synthase (eNOS) mediates nitric oxide (NO) production; polymorphism of the eNOS gene may affect response to the PORH process. This study aims to determine whether eNOS G894T gene polymorphism affects respo...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.05.005
更新日期:2009-09-01 00:00:00
abstract:PURPOSE:To investigate conjunctival microvascular responses in patients with mild diabetic retinopathy (MDR) and methylenetetrahydrofolate reductase (MTHFR) polymorphisms (D + PM) after administration of Ocufolin™, a medical food containing 900 μg l-methylfolate (levomefolate calcium or [6S]-5-methyltetrahydrofolic aci...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104066
更新日期:2020-11-01 00:00:00
abstract::We have applied theories of mass transfer, in laminar and turbulent flow, to red cells in the stopped-flow apparatus in order to estimate the effect of extracellular diffusion boundary layers on the initial rate of O2 uptake. We compared the theoretical predictions with the results of our previous stopped-flow experim...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90058-4
更新日期:1983-07-01 00:00:00
abstract::It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(90)90008-f
更新日期:1990-07-01 00:00:00
abstract::The aim of our study was to evaluate the feasibility of laser Doppler flowmetry (LDF) coupled with iontophoresis in exploring the skin vasodilator activity of exogenous calcitonin gene-related peptide (CGRP) in healthy subjects and to investigate the mechanisms involved in the skin vasodilator activity of this peptide...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2006.10.006
更新日期:2007-03-01 00:00:00
abstract::Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2010.09.005
更新日期:2010-12-01 00:00:00
abstract::Interactions between migratory endothelial cells (ECs) and surrounding extracellular matrix (ECM) are of central importance to vascular growth. Here, we present a new model of EC migration and morphogenesis within three-dimensional ECM termed "radial invasion of matrix by aggregated cells" (RIMAC). In the RIMAC model,...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2122
更新日期:1999-03-01 00:00:00
abstract::This study determined the contribution of cytochrome P450 (CP450) 4A enzyme metabolites of arachidonic acid in mediating the constriction of isolated rat skeletal muscle resistance arteries in response to elevated PO2. Gracilis arteries (GA) were viewed via television microscopy and constrictor responses to elevated P...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2341
更新日期:2001-11-01 00:00:00
abstract::Carrier-immobilized carbohydrates are valuable tools for assessing the glycoligand-binding capacity of cell surfaces. A panel of 10 types of fluorescent neoglycoproteins has been synthesized to determine the extent of their specific binding to endothelial cells in vitro that have been obtained from porcine brain, ovar...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1053
更新日期:1995-09-01 00:00:00
abstract::The relative magnitude of vascular reactivity to inhaled gas stimuli in the major retinal arterioles has not been systematically investigated. The purpose of this study was to compare the magnitude of retinal vascular reactivity in response to inhaled gas provocation at equivalent measurement sites in the superior-, a...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2011.11.002
更新日期:2012-03-01 00:00:00
abstract::Oxygen pressure histograms were determined with the multiwire surface electrode from the gastrocnemius muscle in patients with arterial occlusive disease and compared with those of healthy volunteers. Seven out of 22 patients had normally distributed histograms like volunteers, but the mean PO2 values were lower than ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90024-x
更新日期:1985-05-01 00:00:00
abstract:OBJECTIVE:Microvascular perfusion is indispensable for the growth and remodulation of membrane bone. Trauma, inflammation and surgical interventions may alter periosteal perfusion. However, there is not much known about periosteal perfusion in membrane bones. Therefore, the aim of this study was to establish a new chro...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.09.008
更新日期:2009-03-01 00:00:00
abstract::Local skin heating to 42°C causes rapid increases in cutaneous perfusion (initial peak), followed by a brief nadir and subsequent sustained elevation (plateau). Several studies have demonstrated that nitric oxide synthase (NOS) largely contributes to the plateau response during local heating. In this study, we tested ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2017.12.001
更新日期:2018-05-01 00:00:00
abstract::A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90071-i
更新日期:1991-07-01 00:00:00
abstract::The aim of this study was to analyze the development of vascular architecture as well as vascular morphometry and morphology of anastomosed microvascular free flaps. Free pectoral skin flaps were raised in 25 rats and anastomosed to the femoral vessels in the groin region. CD31 immunohistology was performed after 3, 7...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.03.006
更新日期:2018-07-01 00:00:00
abstract::This study was designed to determine if vascular dysfunction and enhanced norepinephrine sensitivity occurring in early experimental juvenile diabetes (S. M. Mueller, T. M. Mueller, and P. J. Ertel, 1982, Amer. J. Physiol. 243, H139-H144) persist, improve, or worsen in adulthood. Alloxan was administered to rats at 4 ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90030-x
更新日期:1984-07-01 00:00:00
abstract:PURPOSE:Subcutaneous tissue is a promising site for cell transplantation; advantages include minimally invasive procedures and easy post-transplant monitoring. However, limited vascularity is the major known challenge. To address this challenge, a prevascularized graft bed is prepared in recipients. We aimed to establi...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104070
更新日期:2020-11-01 00:00:00
abstract::Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly when fluorescent visu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90022-2
更新日期:1988-11-01 00:00:00
abstract::Strychnine is known to possess anti-inflammatory and antitumour activity, but its roles in tumour angiogenesis, the key step involved in tumour growth and metastasis, and the involved molecular mechanism are still unknown. We aimed to investigate the effects of strychnine on key components of inflammatory angiogenesis...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.01.003
更新日期:2013-05-01 00:00:00
abstract::Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In thi...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.005
更新日期:2018-11-01 00:00:00
abstract::To compute two parameters of capillary protein transport of reflection coefficient (sigma) and permeability--surface area product (PS), two major methods have been employed. The first, or crosspoint method, uses two sets of lymph flow (Jv) and lymph/plasma protein concentration ratios (R) and solves the irreversible t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90051-2
更新日期:1985-09-01 00:00:00
abstract::A hallmark of severe inflammation is reactive oxygen species (ROS) overproduction induced by increased inflammatory mediators secretion. During systemic inflammation, inflammation mediators circulating in the bloodstream interact with endothelial cells (ECs) raising intracellular oxidative stress at the endothelial mo...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.001
更新日期:2015-03-01 00:00:00
abstract::Leptin, a circulating hormone secreted mainly from adipose tissues, is involved in the control of body weight. Recently, leptin was found to be an angiogenic factor, and its vitreous levels are associated with angiogenic eye diseases such as proliferative diabetic retinopathy. However, the molecular mechanism for lept...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00005-0
更新日期:2003-05-01 00:00:00
abstract::Nitrate supplementation in the form of beetroot juice has been shown to increase nitric oxide (NO) where nitrate can be reduced to nitrite and, subsequently, to NO through both nitric oxide synthase (NOS)-dependent and -independent pathways. We tested the hypothesis that nitrate supplementation would augment the NO co...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.12.002
更新日期:2015-03-01 00:00:00