Abstract：
:We have applied theories of mass transfer, in laminar and turbulent flow, to red cells in the stopped-flow apparatus in order to estimate the effect of extracellular diffusion boundary layers on the initial rate of O2 uptake. We compared the theoretical predictions with the results of our previous stopped-flow experiments with suspensions of red cells to which bovine serum albumin (BSA) had been added to decrease O2 solubility and diffusivity (Huxley and Kutchai, 1981). Models of red cells in laminar flow (Friedlander, 1957, 1961; Harriott, 1962) predict a significant retardation of the rate of O2 entry into red cells by diffusion boundary layers. Mixing in the stopped-flow apparatus occurs by convective and turbulent mechanisms in addition to simple molecular diffusion. The more complex theory of mass transfer to particles in turbulent flow (Levich, 1962) shows, because the red cells are highly entrained in the flow, that mixing is not complete on the time scale of initial O2 uptake. The O2 permeability of the diffusion boundary layer, predicted by both laminar and turbulent flow theories, approximates the experimentally obtained value. The theories of mass transfer to particles in laminar flow predict the dependence of the rate of O2 uptake on red cell size observed by other investigators. This suggests that these experimental results may be primarily due to the effect of diffusion boundary layers. Our studies are consistent with the interpretation that red cells in rapid mixing devices may be mixed incompletely with the suspending fluid; thus O2 transfer is limited by molecular diffusion in the immediate vicinity of the erythrocyte. We conclude that the rates of respiratory gas exchange by red cells in flowing systems may be partly limited by diffusion boundary layers.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Huxley VH,Kutchai Hdoi
10.1016/0026-2862(83)90058-4subject
Has Abstractpub_date
1983-07-01 00:00:00pages
89-107issue
1eissn
0026-2862issn
1095-9319journal_volume
26pub_type
杂志文章abstract：:We have performed a mathematical simulation on a CYBER computer of the release, diffusion, and consumption of oxygen in the capillaries and surrounding tissue of peripheral nerve under steady-state conditions. The Krogh-Erlang equation was used to calculate oxygen tension in tissue, while numerical solution of the dif...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(87)90054-9
更新日期：1987-09-01 00:00:00
abstract：:We explored the role of 20-hydroxy-5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid (20-HETE) in oxygen-induced vasoconstriction in a normal renin form of hypertension [the 1 kidney-1 clip Goldblatt hypertensive rat (1K1C)] and a high renin form of hypertension [the 2 kidney-1 clip Goldblatt hypertensive rat (2K1C)]. A silver c...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.09.002
更新日期：2009-12-01 00:00:00
abstract：:We tested the hypothesis that p42/44MAPK and p38MAPK (mitogen-activated protein kinases; MAPK) signaling pathways regulate endothelial cell permeability to macromolecules. Passage 2-4 human umbilical vein endothelial cells (HUVEC) were grown to confluence on fibronectin-coated Snapwell membranes. The flux of fluoresce...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2381
更新日期：2002-03-01 00:00:00
abstract：:Although microvascular dysfunction (MVD) has been well characterized in individual organs as different disease entities, clinical evidence is mounting in support of an underlying systemic process. To address this hypothesis, we systematically searched PubMed and Medline for studies in adults published between 2014 and...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2020.104123
更新日期：2021-03-01 00:00:00
abstract：:The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.008
更新日期：2014-07-01 00:00:00
abstract：:The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(02)00035-3
更新日期：2003-03-01 00:00:00
abstract：:Oxygen pressure histograms were determined with the multiwire surface electrode from the gastrocnemius muscle in patients with arterial occlusive disease and compared with those of healthy volunteers. Seven out of 22 patients had normally distributed histograms like volunteers, but the mean PO2 values were lower than ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90024-x
更新日期：1985-05-01 00:00:00
abstract：:Electrophysiological characteristics of cultured human umbilical vein endothelial cells (HUVEC) were determined using the patch-clamp technique in the whole cell configuration. In isolated cells, membrane potential, capacitance, and input resistance were (Mean +/- SD) - 16.3 +/- 12.7 mV, 53.9 +/- 26 pF, and 2.3 +/- 1....
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1012
更新日期：1994-03-01 00:00:00
abstract：:Using the laser-Doppler method we measured blood flow on the nailfold skin to compare the reactivity of cutaneous microcirculation in three groups of normotensive subjects: 11 subjects with a familial predisposition to hypertension without a previous record of high blood pressure, 6 predisposed subjects with a previou...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2279
更新日期：2000-11-01 00:00:00
abstract：:Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.003
更新日期：2012-09-01 00:00:00
abstract：:Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1016
更新日期：1993-03-01 00:00:00
abstract：:Hyperoxia and hyperbaric oxygen therapy can restore oxygen tensions in tissues distressed by ischemic injury and poor vascularization and is believed to also yield angiogenesis and regulate tissue perfusion. The aim of this study was to develop a model in which hyperoxia-driven microvascular changes could be quantifie...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.02.001
更新日期：2016-05-01 00:00:00
abstract：:The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2236
更新日期：2000-07-01 00:00:00
abstract：:An increasing body of evidence suggests that the deleterious effects of Acute Kidney Injury (AKI) on remote organ function could, at least in part, be due to loss of the normal balance of immune, inflammatory, and soluble mediator metabolism that attends injury of the tubular epithelium. Such dysregulation, acting at ...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2008.09.001
更新日期：2009-01-01 00:00:00
abstract：:This article describes the interaction between solid particles and blood propagating through a capillary. A slip condition is considered on the walls of the capillary. The rheological features of the blood are discussed by considering as a two-phase Newtonian fluid model, i.e., the suspension of cells in plasma. A per...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.04.004
更新日期：2018-09-01 00:00:00
abstract：:Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis in the mammalian eye, suggesting that loss of PEDF is implicated in the pathogenesis of proliferative diabetic retinopathy. However, a role for PEDF in early diabetic retinopathy remains to be elucidated. Since oxidative stress is thought t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.11.001
更新日期：2005-01-01 00:00:00
abstract：:We investigated the kinetics of human mesenchymal stem cells (MSCs) after intravascular administration into SCID mouse cremaster vasculature by intravital microscopy. MSCs were injected into abdominal aorta through left femoral artery at two different concentrations (1 x 10(6) or 0.2 x 10(6) cell). Arterial blood velo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.001
更新日期：2009-05-01 00:00:00
abstract：:Bile duct obstruction is associated with hepatic accumulation of leukocytes and liver injury. Emerging data suggest that platelets may play an important role in tissue damage and inflammation. Herein, we characterized the platelet response in cholestatic liver injury and evaluated the role of Rho-kinase signaling. For...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.04.003
更新日期：2009-06-01 00:00:00
abstract：:Endothelial cells represent one of the critical cellular elements in tumor microenvironment playing a crucial role in the growth and progression of cancer through controlling angiogenesis. Vascular endothelial growth factor (VEGF) produced from tumor cells is essential for the expansion of breast cancer and may functi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.02.008
更新日期：2013-05-01 00:00:00
abstract：:We have used ruthenium red, a cationic dye, to detect at the electron microscopic level the presence of anionic sites in various murine basement membranes, with particular emphasis on those of the microvasculature. We have observed anionic sites in all continuous and fenestrated capillaries examined. Terminal lymphati...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90018-3
更新日期：1983-05-01 00:00:00
abstract：BACKGROUND:In this study we aimed to evaluate effects of liver resection on hepatic microcirculation. In addition we wanted to study if histological liver damage could be detected intra-operatively. PATIENTS AND METHODS:40 patients undergoing hepatic resection were included and grouped according to if they were operat...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.03.007
更新日期：2013-09-01 00:00:00
abstract：:Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerize...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2449
更新日期：2002-11-01 00:00:00
abstract：:The role of VEGF during development and in pathology is well known, but its function in normal adult tissues is poorly understood. Adverse effects associated with the use of anti-angiogenic therapies targeting VEGF in human pathologies have begun to reveal potential functions of VEGF in quiescent vasculature. Further ...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2007.03.004
更新日期：2007-09-01 00:00:00
abstract：:Endothelial dysfunction is common in patients with chronic kidney disease (CKD) and contributes significantly to the high long-term cardiovascular morbidity and mortality. The short-term cardiovascular effects of recombinant human growth hormone (rhGH) in CKD patients (stages III-V) and healthy controls (n=15 each) we...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.05.006
更新日期：2009-09-01 00:00:00
abstract：:Histone acetylation/deacetylation is a key mechanism for regulating transcription, which plays an important role in the control of gene expression, tissue growth, and development. In particular, histone deacetylase 7 (HDAC7), a member of class IIa HDACs, is crucial in maintaining vascular integrity. Endothelial progen...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.10.006
更新日期：2014-01-01 00:00:00
abstract：:The steroid hormone estradiol is suggested to play a protective role in intestinal injury during systemic inflammation (sepsis). Our aim was to determine the effects of specific estradiol receptor (ER-α and ER-ß) agonists on the intestinal microcirculation during experimental sepsis. Male and sham ovariectomized femal...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.10.002
更新日期：2013-01-01 00:00:00
abstract：:The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, howe...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.09.003
更新日期：2018-03-01 00:00:00
abstract：:Homeostasis around vascular endothelium is a function of the equilibrium between the bioavailability of nitric oxide (NO) and oxidizing reactive oxygen species (ROS). Within the vascular endothelium, NO enhances vasodilatation, reduces platelet aggression and adhesion (anti-thrombotic), prevents smooth muscle prolifer...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.03.012
更新日期：2018-09-01 00:00:00
