Abstract:
:The steroid hormone estradiol is suggested to play a protective role in intestinal injury during systemic inflammation (sepsis). Our aim was to determine the effects of specific estradiol receptor (ER-α and ER-ß) agonists on the intestinal microcirculation during experimental sepsis. Male and sham ovariectomized female rats were subjected to sham colon ascendens stent peritonitis (CASP), and they were compared to male and ovariectomized female rats underwent CASP and either estradiol receptor α (ER-α) agonist propyl pyrazole triol (PPT), estradiol receptor ß (ER-ß) agonist diarylpropiolnitrile (DPN), or vehicle treatment. Intravital microscopy was performed, which is sufficiently sensitive to measure changes in the functional capillary density (FCD) as well as the major steps in leukocyte recruitment (rolling and adhesion). The leukocyte extravasations were also quantified by using histological paraffin sections of formalin fixed intestine. We found that either DPN (ER-β) or PPT (ER-α) significantly reduced (P<0.05) sepsis-induced leukocyte-endothelial interaction (rolling, adherent leukocytes and neutrophil extravasations) and improved the intestinal muscular FCD. [PPT: Female; Leukocyte rolling (n/min): V(3) 3.7±0.7 vs 0.8±0.2, Leukocyte adhesion(n/mm(2)): V(3) 131.3±22.6 vs 57.2±13.5, Neutrophil extravasations (n/10000 μm(2)): 3.1±0.7 vs 6 ±1. Male; Leukocyte adhesion (n/mm(2)): V(1) 154.8±19.2 vs 81.3±11.2, V(3) 115.5±23.1 vs 37.8±12]. [DPN: Female; neutrophil extravasations (n/10000 μm(2)) 3.8±0.6 vs 6 ±1. Male; Leukocyte adhesion (n/mm(2)) V(1) 154.8±19.2 vs 70±10.5, V(3) 115.5±23.1 vs 52.8±9.6].Those results suggest that the observed effects of estradiol receptors on different phases of leukocytes recruitment with the improvement of the functional capillary density could partially explain the previous demonstrated salutary effects of estradiol on the intestinal microcirculation during sepsis. The observed activity of this class of compounds could open up a new avenue of research into the potential treatment of sepsis.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Sharawy N,Ribback S,Al-Banna N,Lehmann C,Kern H,Wendt M,Cerny V,Dombrowski F,Pavlovic Ddoi
10.1016/j.mvr.2012.10.002subject
Has Abstractpub_date
2013-01-01 00:00:00pages
118-27eissn
0026-2862issn
1095-9319pii
S0026-2862(12)00165-3journal_volume
85pub_type
杂志文章abstract:OBJECTIVE:The relationship between NF-κB Interacting lncRNA (NKILA) and angiogenesis in breast cancer has never been studied. Our study aimed to investigate effect of NKILA on proliferation, migration, apoptosis, as well as angiogenesis in breast cancer. METHODS:NKILA was over-expressed in MDA-MB-231 cells by transfec...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.103968
更新日期:2020-05-01 00:00:00
abstract::Mesangium enlargement is a central feature of diabetic nephropathy and almost certainly plays a pathogenic role in this condition. Previous studies have shown that mesangium degradation is reduced in a high glucose mileau. Plasmin has been shown to play an important role in extracellular matrix degradation, both direc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1996.2006
更新日期:1997-03-01 00:00:00
abstract::We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title:Microvascular research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) was originally discovered as vascular permeability factor because of its ability to increase microvascular permeability to plasma proteins. Since then, it has been shown to induce proliferation and migration in endothelial cells. Placenta growth factor (PlGF) is a member of th...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2298
更新日期:2001-03-01 00:00:00
abstract::The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1997.2043
更新日期:1997-11-01 00:00:00
abstract::An ex vivo perfused solid tumor preparation provides control over the physiological, biochemical, and pharmacological composition of the arterial input and easy access to the venous output. This is advantageous for studies of transport and metabolism in solid tumors. Here we present a tissue-isolated tumor preparation...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1063
更新日期:1994-11-01 00:00:00
abstract::Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.07.006
更新日期:2009-12-01 00:00:00
abstract::We determined whether the solvent drag reflection coefficient (sigma f) for total proteins of a canine perfused left lower lung lobe (LLL) preparation decreases at elevated venous pressures (Pv). We found that sigma f (estimated using the hematocrit-protein technique) remained constant at all Pv's (30-95 mm Hg) evalua...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(92)90006-b
更新日期:1992-01-01 00:00:00
abstract::The effect of mean blood velocity on thrombus formation parameters was studied using mesenteric vessels of white rats. An advanced phenomenological theory, explaining the relationship between mean blood velocity and thrombus growth rate, is presented. The qualitative analysis of theoretical and experimental data have ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1002
更新日期:1995-01-01 00:00:00
abstract::Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of ne...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.12.001
更新日期:2015-03-01 00:00:00
abstract::In a novel assay of angiogenesis in the quail chorioallantoic membrane (CAM), we measured vascular pattern and angiogenic rate after homogeneous exposure of the entire vascular tree to recognized modulators of vessel growth. In comparison to phosphate-buffered saline (PBS)-treated controls, the vascular stimulator, ba...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2073
更新日期:1998-05-01 00:00:00
abstract::Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104103
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Tissue ischemia and reperfusion (I/R) affects blood flow restoration and oxygen delivery to the damaged tissues contributing to tissue morbidity and microcirculatory compromise. Pulsed acoustic cellular expression (PACE) technology is known to support tissue neovascularization. The aim of this study was to t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.11.005
更新日期:2012-03-01 00:00:00
abstract::Thrombolysis treatment of acute ischemic stroke is limited by the pro-edematous and hemorrhagic effects exerted by reperfusion, which disrupts the blood-brain barrier (BBB) capillary endothelium in the infarct core. Most studies of the ischemic BBB overlook the complexity of the penumbral area, where the affected brai...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.12.002
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Hypertension and hypercholesterolemia elicit inflammatory and thrombogenic responses in the microvasculature. However, little is known about whether and how risk factor combinations alter microvascular function. We examined how the actions of HTN+HCh on the microvasculature differ from the responses elicited ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2016.01.002
更新日期:2016-05-01 00:00:00
abstract::Age-related macular degeneration (AMD) is a common cause of blindness worldwide. While recent studies have revealed that the loss of choroidal endothelial cells (ChECs) is critical to the disease pathogenesis of dry AMD, in vitro studies are needed to fully elucidate the disease mechanism. However, these studies remai...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.12.002
更新日期:2019-05-01 00:00:00
abstract::Pulmonary arterial hypertension is a common complication of congenital heart defects with left-to-right shunts. Current preclinical models do not reproduce clinical characteristics of shunt-related pulmonary hypertension. Aorto-caval shunt was firstly described as a model of right ventricle volume overload. The pathop...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.05.005
更新日期:2014-07-01 00:00:00
abstract::Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.03.001
更新日期:2010-07-01 00:00:00
abstract::The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2236
更新日期:2000-07-01 00:00:00
abstract::Hypoxia-induced arginase elevation plays an essential role in several vascular diseases but influence of arginase on hypoxia-mediated angiogenesis is completely unknown. In this study, in vitro network formation in bovine aortic endothelial cells (BAEC) was examined after exposure to hypoxia for 24h with or without ar...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.11.002
更新日期:2015-03-01 00:00:00
abstract::Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.11.002
更新日期:2014-01-01 00:00:00
abstract::Visualization of the lymphatic system is clinically necessary during diagnosis or treatment of many conditions and diseases; it is used for identifying and monitoring lymphedema, for detecting metastatic lesions during cancer staging and for locating lymphatic structures so they can be spared during surgical procedure...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2014.06.006
更新日期:2014-11-01 00:00:00
abstract::Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2010.09.005
更新日期:2010-12-01 00:00:00
abstract::Previously reported cell-flow microchannels in a single-crystal silicon substrate (Microvasc. Res. 44, 226-240, 1992) have been modified, and flow behavior of blood cells is described using flow rate-time curves and video pictures. The principal structure (2600 identically sized channels in parallel) was retained to g...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1008
更新日期:1994-01-01 00:00:00
abstract::It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(90)90008-f
更新日期:1990-07-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) and their receptors play important roles in cellular processes such as proliferation, differentiation, migration and cell survival. It was also demonstrated that BMPs are involved in vasculogenesis and angiogenesis. In this study, we investigated the expression profile of BMP recepto...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.03.010
更新日期:2012-07-01 00:00:00
abstract::The aim of this study is to develop a novel non-contrast 4-dimensional MR arterial spin labeling (4D-ASL) technique (3D acquisition and time) and to investigate myocardial perfusion on healthy volunteers without administration of contrast materials. A non-contrast 4D-ASL technique was developed using the time-spatial ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.01.007
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.10.008
更新日期:2014-03-01 00:00:00
abstract::Blood flow studies in the brain, heart, and other organs suggest that there could be interaction between nitric oxide (NO) and adenosine. This possibility was investigated in the optic nerve head (ONH) during photic stimulation of the dark-adapted cat eye. Functional activation of ONH blood flow was measured by laser ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2002.2432
更新日期:2002-09-01 00:00:00
abstract::Lipopolysaccharide released during bacterial sepsis causes acute lung injury and ARDS. Pulmonary microvascular injury is a feature of ARDS, and vascular remodeling develops, leading to pulmonary hypertension. Pericytes in the lung circulation proliferate and contribute to the remodeling seen in experimental sepsis. It...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2085
更新日期:1998-09-01 00:00:00