Abstract:
OBJECTIVE:The relationship between NF-κB Interacting lncRNA (NKILA) and angiogenesis in breast cancer has never been studied. Our study aimed to investigate effect of NKILA on proliferation, migration, apoptosis, as well as angiogenesis in breast cancer. METHODS:NKILA was over-expressed in MDA-MB-231 cells by transfection of pcDNA3.1-NKILA vector. Cell viability, apoptosis and migration were measured by MTT, flow cytometry and wound healing assays, respectively. Angiogenesis of human umbilical vein endothelial cells (HUVEC) was measured using tube formation assay. The expression levels of NKILA, IL-6, VEGFA, VEGFR, apoptosis and epithelial-mesenchymal transition (EMT) and NF-κB/IL-6 signaling-related markers were determined using qRT-PCR or Western blotting. RESULTS:Cell viability and migration of MDA-MB-231 cells were significantly inhibited, while cell apoptosis was obviously promoted by overexpression of NKILA. Overexpression of NKILA could also inhibit the phosphorylation of IκBα and the nuclear transposition of p65, as well as induce cell apoptosis-related proteins and inhibit epithelial-mesenchymal transition-related proteins. Cell viability and migration of HUVEC were also significantly inhibited when treated with supernatant of cells overexpressed NKILA or treated with BAY11-7028. Exogenous IL-6 significantly increased the cell viability and migration of HUVEC, and overexpression of NKILA could reverse these effects induced by IL-6. Overexpression of NKILA significantly inhibited the protein levels of IL-6 and VEGFA in supernatant, as well as VEGFR in HUVEC, thus inhibited the angiogenesis of HUVEC. NKILA also reversed the above effects on protein levels of IL-6 and VEGFA in supernatant and angiogenesis induced by exogenous IL-6. CONCLUSION:Overexpression of NKILA could inhibit cell proliferation, migration and promote apoptosis of breast cancer cells. It could also inhibit cell proliferation, migration and angiogenesis of HUVEC through inhibiting IL-6 secretion via NF-κB signaling pathway.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Luo LH,Rao L,Luo LF,Chen K,Ran RZ,Liu XLdoi
10.1016/j.mvr.2019.103968subject
Has Abstractpub_date
2020-05-01 00:00:00pages
103968eissn
0026-2862issn
1095-9319pii
S0026-2862(19)30218-3journal_volume
129pub_type
杂志文章abstract:BACKGROUND:Angiogenin levels are increased in children and adolescent patients with type 1 diabetes, regardless of the extent of diabetic microangiopathy. However, little is known about the angiogenin concentrations in adults with type 1 diabetes. Thus we studied its level in middle aged subjects with the presence of d...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.08.005
更新日期:2012-11-01 00:00:00
abstract::A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green)...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.11.012
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVES:Calcium channel inhibitors have beneficial impact on microcirculation, but beta-blocker effect is controversial. Clinicians still do not agree on beta-blocker combination with other treatments in the management of impaired microcirculation. The aim of the present study was to describe the effects of beta-blo...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.04.004
更新日期:2011-07-01 00:00:00
abstract::Thrombolysis treatment of acute ischemic stroke is limited by the pro-edematous and hemorrhagic effects exerted by reperfusion, which disrupts the blood-brain barrier (BBB) capillary endothelium in the infarct core. Most studies of the ischemic BBB overlook the complexity of the penumbral area, where the affected brai...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.12.002
更新日期:2014-01-01 00:00:00
abstract::Oxidative stress, which is defined as an imbalance between proxidant and antioxidant systems, is the essential mechanism involving in the ischemic process. During the early stage of brain ischemia, reactive oxygen species (ROS) are increased. Increased ROS are thought of a consequence of brain ischemia and exacerbatin...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2018.12.005
更新日期:2019-05-01 00:00:00
abstract::Capillary diameters in sartorius muscle of frogs were measured in vivo by means of a new computer video method, based on the passage of red blood cells (RBCs) through the capillary (C. Ellis, R. Sanfranyos, and A. Groom (1983), Microvasc. Res. 26, 139-150). The distribution of capillary diameters from 21 frogs was rep...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90066-3
更新日期:1983-09-01 00:00:00
abstract::We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2017.04.003
更新日期:2017-09-01 00:00:00
abstract::The purpose of this study was to elucidate the mechanism by which bradykinin (BK) enhances prostacyclin (PGI(2)) production in human umbilical vein endothelial cells (HUVEC). BK-induced enhancement of PGI(2) synthesis was observed in a dose- and time-dependent manner, and it also increased [Ca(2+)](i) followed by enha...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2163
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Impairments at the microvascular level might lead to more overt cardiovascular complications, therefore, being able to early detect microvascular dysfunction would be beneficial. Thus, the present study investigated whether near-infrared spectroscopy (NIRS) assessment of microvascular responsiveness (reoxyge...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.04.009
更新日期:2019-09-01 00:00:00
abstract::Previously reported cell-flow microchannels in a single-crystal silicon substrate (Microvasc. Res. 44, 226-240, 1992) have been modified, and flow behavior of blood cells is described using flow rate-time curves and video pictures. The principal structure (2600 identically sized channels in parallel) was retained to g...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1008
更新日期:1994-01-01 00:00:00
abstract::Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.06.011
更新日期:2012-11-01 00:00:00
abstract::Routine pulmonary ultrasound for diagnosis of disease or injury relies on interpretation of image features, such as comet-tail artifacts, which can also be indicative of the poorly understood phenomenon of ultrasound-induced pulmonary capillary hemorrhage (PCH). Evans blue extraction and bronchoalveolar lavage (BAL) w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::Using the laser-Doppler method we measured blood flow on the nailfold skin to compare the reactivity of cutaneous microcirculation in three groups of normotensive subjects: 11 subjects with a familial predisposition to hypertension without a previous record of high blood pressure, 6 predisposed subjects with a previou...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2279
更新日期:2000-11-01 00:00:00
abstract::We determined whether the solvent drag reflection coefficient (sigma f) for total proteins of a canine perfused left lower lung lobe (LLL) preparation decreases at elevated venous pressures (Pv). We found that sigma f (estimated using the hematocrit-protein technique) remained constant at all Pv's (30-95 mm Hg) evalua...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(92)90006-b
更新日期:1992-01-01 00:00:00
abstract::The relative magnitude of vascular reactivity to inhaled gas stimuli in the major retinal arterioles has not been systematically investigated. The purpose of this study was to compare the magnitude of retinal vascular reactivity in response to inhaled gas provocation at equivalent measurement sites in the superior-, a...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2011.11.002
更新日期:2012-03-01 00:00:00
abstract::The contribution of the pleural mesothelium to pleural liquid and protein transport is still vigorously debated. Recent in vitro studies of stripped pleural membrane and free-standing pericardium have demonstrated active ion solute coupled transport of liquid and transcytosis of protein. However, the relative contribu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2005.10.003
更新日期:2005-11-01 00:00:00
abstract::Skeletal muscle arterioles are known to constrict upon elevation of ambient PO2. While several studies have shown that the endothelium plays an important role in this response, it is not clear how this response is mediated. We examined the oxygen-induced constriction of arterioles in the rat spinotrapezius muscle. Ele...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1025
更新日期:1995-05-01 00:00:00
abstract::We have developed an intensity analysis technique for fluorescence microscopy that allows us to measure, in real time, the diameter and the membrane potential or intracellular calcium ([Ca(2+)]i) of in vivo arteriolar endothelium or smooth muscle. Cheek pouch arterioles of anesthetized hamsters were luminally or ablum...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2315
更新日期:2001-07-01 00:00:00
abstract::A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90058-1
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND:Sedentary behaviour is linked to increased cardiovascular disease risk in Rheumatoid Arthritis (RA), but the biological processes underlying this relationship are not understood. OBJECTIVES:To investigate the cross-sectional associations of habitual sedentary behaviour, with endothelial function in RA. MET...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.01.005
更新日期:2018-05-01 00:00:00
abstract::A theoretical model, appropriate for data interpretation, is presented for the transcapillary fluid exchange and associated intraluminal hydrodynamic and solute transport occurring in the microocclusion of single capillaries. This analysis describes the spatial and temporal behavior of the intraluminal flow. A compari...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90012-2
更新日期:1983-03-01 00:00:00
abstract::The effect of hemodilution on oxygen delivery to the tissue was investigated analytically by taking into consideration the oxygen loss that occurs along the arterial microvasculature due to diffusion into the tissue and shunting to parallel running venules. The theoretical findings were related to experimental data on...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(87)90062-8
更新日期:1987-11-01 00:00:00
abstract:OBJECTIVE:Studies on the acute effects of coffee on the microcirculation have shown contradicting results. This study aimed to investigate if intake of caffeine-containing coffee changes blood flow and microvascular reactivity in the skin. METHODS:We measured acute changes in cutaneous vascular conductance (CVC) in th...
journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2017.06.006
更新日期:2017-11-01 00:00:00
abstract::In this paper we quantitatively investigate the hypothesis proposed by Michel (Exp. Physiol. 82, 1-30, 1997) and Weinbaum (Ann. Biomed. Eng. 26, 1-17, 1998) that the Starling forces are determined by the local difference in the hydrostatic and colloid osmotic pressure across the endothelial surface glycocalyx, which w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2177
更新日期:1999-11-01 00:00:00
abstract::A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90071-i
更新日期:1991-07-01 00:00:00
abstract::In diabetic patients small fiber neuropathy has been associated with impairment of 0.1 Hz microvascular vasomotion. The aim of this study was (1) to investigate whether vasoconstriction-induced microvascular oscillations in the skin are reduced in diabetic patients with peripheral and/or autonomic neuropathy, and (2) ...
journal_title:Microvascular research
pub_type: 临床试验,杂志文章
doi:10.1016/j.mvr.2008.04.004
更新日期:2008-08-01 00:00:00
abstract::We tested the hypothesis that p42/44MAPK and p38MAPK (mitogen-activated protein kinases; MAPK) signaling pathways regulate endothelial cell permeability to macromolecules. Passage 2-4 human umbilical vein endothelial cells (HUVEC) were grown to confluence on fibronectin-coated Snapwell membranes. The flux of fluoresce...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2381
更新日期:2002-03-01 00:00:00
abstract::Polyphenolic compounds (anthocyanins, flavonoid glycosides) in berries prevent the initiation, promotion, and progression of carcinogenesis in rat's digestive tract and esophagus, in part, via anti-inflammatory pathways. Angiogenesis has been implicated in the pathogenesis of chronic inflammation and tumorigenesis. In...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.10.008
更新日期:2015-01-01 00:00:00
abstract::The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(02)00035-3
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.10.008
更新日期:2014-03-01 00:00:00