Abstract：
:We have developed an intensity analysis technique for fluorescence microscopy that allows us to measure, in real time, the diameter and the membrane potential or intracellular calcium ([Ca(2+)]i) of in vivo arteriolar endothelium or smooth muscle. Cheek pouch arterioles of anesthetized hamsters were luminally or abluminally labeled with Di-8-ANEPPS, a voltage-sensitive dye, or Fura PE3, a calcium indicator. The peak fluorescence intensities of the images were used to locate the endothelium or smooth muscle. The changes in membrane potential or [Ca(2+)]i were determined based on the ratiometric analysis of fluorescence intensity of the endothelium or smooth muscle. Membrane depolarization of the smooth muscle using KCl caused a decrease in the ratio of emission, 620 nm/560 nm ( approximately 6 mV/% ratio). The ratio of excitation, 340 nm/380 nm, increased with increasing free Ca(2+). Methacholine, a muscarinic receptor agonist, caused arteriolar dilation (12.2 +/- 0.9 µm). It produced hyperpolarization of the endothelium and smooth muscle (2.8 +/- 0.6% and 2.3 +/- 0.3% in ratio). Methacholine also induced an increase in [Ca(2+)]i (11.0 +/- 1.1% in ratio) of the endothelium. In contrast, methacholine caused a biphasic change in [Ca(2+)]i of the smooth muscle, a rapid reduction (-3.4 +/- 0.2% in ratio) followed by a prolonged increase (2.4 +/- 0.2% in ratio). These results demonstrate that the peak intensity analysis can be used to determine in real time the changes in membrane potential or [Ca(2+)]i of in vivo endothelium or smooth muscle.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Chen Y,Rivers RJdoi
10.1006/mvre.2001.2315keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
55-62issue
1eissn
0026-2862issn
1095-9319pii
S0026-2862(01)92315-5journal_volume
62pub_type
杂志文章abstract：PURPOSE:Subcutaneous tissue is a promising site for cell transplantation; advantages include minimally invasive procedures and easy post-transplant monitoring. However, limited vascularity is the major known challenge. To address this challenge, a prevascularized graft bed is prepared in recipients. We aimed to establi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104070
更新日期：2020-11-01 00:00:00
abstract：:Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1016
更新日期：1993-03-01 00:00:00
abstract：:Although vasodilation is conventionally held to be the predominant microvascular response to a wound, there has been no previous attempt to actually quantitate skin blood flow within and in the neighborhood of wounds. In particular, there has been no differentiation between sites with primarily nutritive (NUTR) blood ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2008
更新日期：1997-05-01 00:00:00
abstract：:We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.04.003
更新日期：2017-09-01 00:00:00
abstract：:A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90071-i
更新日期：1991-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2177
更新日期：1999-11-01 00:00:00
abstract：:In previous studies, the flow of albumin solution through hydrated lung interstitial segments was higher than a prior flow of Ringer solution (A. Tajaddinni et al., 1994, J. Appl. Physiol. 76, 578-583). We wondered whether this effect was caused by an increased pore size. We measured the flow of albumin solutions thro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2363
更新日期：2002-01-01 00:00:00
abstract：:Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In thi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.05.005
更新日期：2018-11-01 00:00:00
abstract：:Lipopolysaccharide released during bacterial sepsis causes acute lung injury and ARDS. Pulmonary microvascular injury is a feature of ARDS, and vascular remodeling develops, leading to pulmonary hypertension. Pericytes in the lung circulation proliferate and contribute to the remodeling seen in experimental sepsis. It...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2085
更新日期：1998-09-01 00:00:00
abstract：:This paper presents the results of a circulation analysis using an image based network model of a murine retinal vasculature, which closely represents the 3D vascular distribution of the retina. The uneven distribution of the red blood cells at vascular network bifurcations (i.e., plasma skimming effect), the microvas...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.02.005
更新日期：2010-07-01 00:00:00
abstract：:The fluidity (the inverse of viscosity) of red blood cell (RBC) suspensions in vivo was estimated by means of microcirculatory measurements such as RBC flow velocity, micropressure in arterioles and venules, and vessel geometry, and by means of simultaneous macrocirculatory measurements such as volume flow and perfusi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(88)90051-9
更新日期：1988-01-01 00:00:00
abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2043
更新日期：1997-11-01 00:00:00
abstract：:The study of angiogenesis as a therapeutic target requires reliable in vivo assays that can provide physiologically relevant data. A murine in vivo Matrigel-based angiogenesis assay is presented here which includes the quantitative assessment of vascular-specific indicators of neovascularization. Matrigel containing 1...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.01.002
更新日期：2006-03-01 00:00:00
abstract：:Angiotensin II is considered to have angiogenic properties. Nevertheless, several authors reported an increase in coronary capillary density after treatment with ACE inhibitors. The aim of the present study was to evaluate the effect of treatment with low doses of ACE inhibitor perindopril, low doses of the diuretic i...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2224
更新日期：2000-03-01 00:00:00
abstract：:Post occlusive skin reactive hyperemia (PORH) is a tool used to assess microcirculation. Endothelial nitric oxide synthase (eNOS) mediates nitric oxide (NO) production; polymorphism of the eNOS gene may affect response to the PORH process. This study aims to determine whether eNOS G894T gene polymorphism affects respo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.05.005
更新日期：2009-09-01 00:00:00
abstract：:Previously reported cell-flow microchannels in a single-crystal silicon substrate (Microvasc. Res. 44, 226-240, 1992) have been modified, and flow behavior of blood cells is described using flow rate-time curves and video pictures. The principal structure (2600 identically sized channels in parallel) was retained to g...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1008
更新日期：1994-01-01 00:00:00
abstract：:The microfibrils of anchoring filaments, a typical ultrastructural feature of initial lymphatic vessels, consist mainly of fibrillin and are similar to the microfibrils of elastic fibers. As we previously demonstrated, they radiate from focal adhesions of lymphatic endothelium to the perivascular elastic network. Alth...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2397
更新日期：2002-07-01 00:00:00
abstract：:Nonspecific vasodilatation during iontophoresis is an important confounding factor in experimental pharmacology. In this investigation, we studied the involvement of sensory nerves and histamine-related reactions in causing nonspecific vasodilatation in a model of anodal and cathodal iontophoresis of sodium chloride. ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2003.12.002
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:The relationship between NF-κB Interacting lncRNA (NKILA) and angiogenesis in breast cancer has never been studied. Our study aimed to investigate effect of NKILA on proliferation, migration, apoptosis, as well as angiogenesis in breast cancer. METHODS:NKILA was over-expressed in MDA-MB-231 cells by transfec...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103968
更新日期：2020-05-01 00:00:00
abstract：:The size of hyaluronan in lymph and gastrocnemius muscle was measured to test the hypothesis that reperfusion of ischemic skeletal muscle leads to hyaluronan depolymerization. Prenodal lymph was collected from the gastrocnemius muscle in anesthetized rabbits. Hyaluronan size was measured using a combination of agarose...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2437
更新日期：2002-09-01 00:00:00
abstract：:A hallmark of severe inflammation is reactive oxygen species (ROS) overproduction induced by increased inflammatory mediators secretion. During systemic inflammation, inflammation mediators circulating in the bloodstream interact with endothelial cells (ECs) raising intracellular oxidative stress at the endothelial mo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.02.001
更新日期：2015-03-01 00:00:00
abstract：:There is evidence that vascular beds distal to the ophthalmic artery (OA) show vasoconstriction in response to a step decrease in systemic blood pressure (BP). The mediators of this response are mostly unidentified. The aim of the current study was to test the hypothesis that α2-adrenoreceptors may contribute to the r...
journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2010.10.001
更新日期：2011-01-01 00:00:00
abstract：:Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.011
更新日期：2012-11-01 00:00:00
abstract：:We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title：Microvascular research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.008
更新日期：2014-07-01 00:00:00
abstract：:The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90040-8
更新日期：1985-07-01 00:00:00
abstract：:Recently the zebrafish has emerged as a promising vertebrate model of lymphatic vasculature development. The establishment of numerous transgenic lines that label the lymphatic endothelium in the zebrafish has allowed the fine examination of the developmental timing and the anatomy of their lymphatic vasculature. Alth...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2014.06.001
更新日期：2014-11-01 00:00:00
abstract：:The purpose of the present study was to investigate the nervous control of gingival blood flow in cats. Gingival blood flow was measured by laser Doppler flowmeter in 75 cats during electrical stimulation and cutting or ligation of the inferior alveolar nerve and cervical sympathetic nerve without sympathectomy or pre...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90061-u
更新日期：1990-01-01 00:00:00
abstract：:Angiogenesis, or the growth of new blood vessels, is critical to both health and disease. Several genes are implicated in the regulation of angiogenesis, such as genes encoding growth factors, cytokines, and the extracellular matrix, as well as transcription factors. Among them, Hox genes exert master regulatory effec...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2013.02.009
更新日期：2013-05-01 00:00:00
abstract：:Oxidative stress, which is defined as an imbalance between proxidant and antioxidant systems, is the essential mechanism involving in the ischemic process. During the early stage of brain ischemia, reactive oxygen species (ROS) are increased. Increased ROS are thought of a consequence of brain ischemia and exacerbatin...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.12.005
更新日期：2019-05-01 00:00:00
