Abstract:
:The study of angiogenesis as a therapeutic target requires reliable in vivo assays that can provide physiologically relevant data. A murine in vivo Matrigel-based angiogenesis assay is presented here which includes the quantitative assessment of vascular-specific indicators of neovascularization. Matrigel containing 175 ng/ml bFGF is encapsulated in synthetic chambers which are implanted subcutaneously in C57/B16J mice. Ex vivo implants can be imaged to qualitatively view perfused vasculature within the chambers, or histologically processed to confirm the presence of vascular-specific tissue within the Matrigel. Viable cells are recovered from the excised chambers and quantified cytometrically using endothelial cell-specific markers CD34 and CD144, and for a marker of nucleated cells, Hoechst 33342. Thalidomide, 200 mg/kg/day, was tested using the assay and was found to inhibit angiogenesis by 46%. Angiogenesis inhibitors secreted by LL/M27 tumors were also characterized, where tumor-bearing mice showed a 73% inhibition of angiogenesis compared to tumor-free controls. Analysis of the number of nucleated cells in these samples failed to show a strong correlation with the number of endothelial cells, indicating that quantification of nonvascular-specific tissue in in vivo angiogenesis assays may not be sufficient. This new assay provides an objective, comprehensive determination of the vasculature-specific response of both endogenous and exogenous angiogenesis inhibitors in vivo, and also creates new opportunities for obtaining primary murine endothelial cells.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Baker JH,Huxham LA,Kyle AH,Lam KK,Minchinton AIdoi
10.1016/j.mvr.2006.01.002keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
69-75issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(06)00016-1journal_volume
71pub_type
杂志文章abstract::Patients with diabetic polyneuropathy are known to have an impaired neurovascular reflex arc compared to healthy controls. This is seen in a delayed decrease in microcirculation of the ipsilateral hand after cooling of the contralateral hand. The aim of this pilot study was to investigate whether intravenous alpha-lip...
journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1996.0047
更新日期:1996-09-01 00:00:00
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doi:10.1016/j.mvr.2003.12.002
更新日期:2004-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 临床试验,杂志文章
doi:10.1016/j.mvr.2008.04.004
更新日期:2008-08-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2008.10.005
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doi:10.1016/j.mvr.2012.03.010
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doi:10.1006/mvre.2000.2259
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journal_title:Microvascular research
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