Abstract：
:We examined the impact of lymph flow obstruction in large post-nodal lymphatic vessels in sheep. A silk ligature was placed 2 cm downstream from the prescapular or popliteal lymph node and tightened to interrupt flow. At 6, 12 and 16 weeks after lymph flow blockage, a network of small interconnecting lymphatics (approximately 10-40 microm in diameter) could be observed in the vicinity of the ligature. These were identified using antibodies to the lymphatic endothelial markers LYVE-1 or VEGFR-3 or unequivocally, with the upstream intraluminal injection of the non-specific cell dye CFDA-SE. The observed lymphangiogenesis coincided with increased levels of Prox1, Tie2 (Y992) phosphorylation, MAPK activation, and decreased Akt activition. In the popliteal preparations, saline was infused into the prenodal ducts upstream of the regeneration site. The slopes of the inflow pressure versus flow relationships were 17.3+/-3.6, immediately after vessel obstruction, 36.2+/-9.6 at 6 weeks and 15.0+/-5.3 at 12-16 weeks. For comparison, the average slope in a completely intact popliteal system was 3.1+/-0.3 (from a previous publication). The resistance to flow remained high up to 12-16 weeks after flow obstruction suggesting that normal flow parameters had not been achieved over this time. The lymph node appeared to have some role in limiting the impact of post-nodal lymph obstruction, a function that appeared to be compromised by lymph stasis.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Jila A,Kim H,Nguyen VP,Dumont DJ,Semple J,Armstrong D,Seto E,Johnston Mdoi
10.1016/j.mvr.2006.11.003subject
Has Abstractpub_date
2007-05-01 00:00:00pages
214-23issue
3eissn
0026-2862issn
1095-9319pii
S0026-2862(06)00131-2journal_volume
73pub_type
杂志文章abstract：:We have developed a simplified technique for culturing human umbilical vein endothelial cells under shear flow conditions, using prefabricated glass microcapillary tubes ("microslides") with a well-defined rectangular cross section and good optical quality. These microslides have been incorporated into a controlled fl...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1004
更新日期：1993-01-01 00:00:00
abstract：:In preeclampsia, invasion of intrauterine decidual blood vessels by placental cytotrophoblasts is significantly reduced. This study examined the secretion of matrix metalloproteinases (MMP) by cultured human decidual endothelial cells from normal (NDEC) and preeclamptic (PEDEC) pregnancies. MMPs secreted into the cult...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2142
更新日期：1999-05-01 00:00:00
abstract：:Patients with diabetic polyneuropathy are known to have an impaired neurovascular reflex arc compared to healthy controls. This is seen in a delayed decrease in microcirculation of the ipsilateral hand after cooling of the contralateral hand. The aim of this pilot study was to investigate whether intravenous alpha-lip...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2151
更新日期：1999-07-01 00:00:00
abstract：:Failure of the glomerular barrier causing proteinuria has been modeled chiefly by Chang, Deen, and Brenner. They have refined models from an isoporous filter to a mostly isoporous membrane, which during proteinuric disease opens up nondiscriminating shunts. This report extends these concepts by measuring a larger dist...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2146
更新日期：1999-05-01 00:00:00
abstract：:During diabetes in rats, the choroid of the eye shows increased permeability to albumin, basement membrane thickening, and decreased anionic charge sites on the abluminal surfaces of the choriocapillary microvessels. In other microvascular beds, permeability differences are correlated with differences in luminal membr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90058-j
更新日期：1991-11-01 00:00:00
abstract：:Systemic sclerosis (SSc) is a connective tissue disease with high morbidity and mortality, characterized by autoimmunity, obliterative vasculopathy involving mainly the microvasculature, and fibrosis. SSc-specific nailfold capillaroscopic changes have been defined, and nailfold capillaroscopy (NFC) is now unequivocall...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.03.004
更新日期：2018-07-01 00:00:00
abstract：:Polyphenolic compounds (anthocyanins, flavonoid glycosides) in berries prevent the initiation, promotion, and progression of carcinogenesis in rat's digestive tract and esophagus, in part, via anti-inflammatory pathways. Angiogenesis has been implicated in the pathogenesis of chronic inflammation and tumorigenesis. In...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.10.008
更新日期：2015-01-01 00:00:00
abstract：:Angiogenesis, or the growth of new blood vessels, is critical to both health and disease. Several genes are implicated in the regulation of angiogenesis, such as genes encoding growth factors, cytokines, and the extracellular matrix, as well as transcription factors. Among them, Hox genes exert master regulatory effec...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2013.02.009
更新日期：2013-05-01 00:00:00
abstract：:We tested the hypothesis that the endothelial nitric oxide (NO)-soluble guanylyl cyclase system is involved in atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) mediated regulation of coronary and peripheral vascular resistance. Rat hearts were perfused via the aorta at constant flow and the effect...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2289
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:Diabetic retinopathy (DR) is characterized by pro-inflammatory, pro-angiogenic and pro-fibrotic environment during the various stages of the disease progression. Basement membrane changes in the retina and formation of fibrovascular membrane are characteristically seen in DR. In the present study the effect of ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.09.001
更新日期：2019-01-01 00:00:00
abstract：:Changes in blood vessel diameter can be measured manually, but this is time-consuming and often impractical. For automatic measurement commercial solutions are available, but the proprietary algorithms and their potential shortcomings are not known to the user. We present an approach with a CCD camera for image acquis...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.04.004
更新日期：2010-09-01 00:00:00
abstract：:In the present study, human myometrial microvascular endothelial cells (HMMEC) were cocultured with human vascular smooth muscle cells (VSMC) labeled with fluorescent dyes to examine their morphological interaction using confocal laser fluorescent microscopy. HMMEC and VSMC labeled with fluorescent green and red dyes,...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.008
更新日期：2005-07-01 00:00:00
abstract：:The purpose of this study was to elucidate the mechanism by which bradykinin (BK) enhances prostacyclin (PGI(2)) production in human umbilical vein endothelial cells (HUVEC). BK-induced enhancement of PGI(2) synthesis was observed in a dose- and time-dependent manner, and it also increased [Ca(2+)](i) followed by enha...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2163
更新日期：1999-09-01 00:00:00
abstract：:The relative magnitude of vascular reactivity to inhaled gas stimuli in the major retinal arterioles has not been systematically investigated. The purpose of this study was to compare the magnitude of retinal vascular reactivity in response to inhaled gas provocation at equivalent measurement sites in the superior-, a...
journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2011.11.002
更新日期：2012-03-01 00:00:00
abstract：:This study determined the contribution of cytochrome P450 (CP450) 4A enzyme metabolites of arachidonic acid in mediating the constriction of isolated rat skeletal muscle resistance arteries in response to elevated PO2. Gracilis arteries (GA) were viewed via television microscopy and constrictor responses to elevated P...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2341
更新日期：2001-11-01 00:00:00
abstract：:Purine nucleosides and nucleobases play key roles in the physiological response to vascular ischemia/reperfusion events. The intra- and extracellular concentrations of these compounds are controlled, in part, by equilibrative nucleoside transporter subtype 1 (ENT1; SLC29A1) and by equilibrative nucleobase transporter ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.06.008
更新日期：2014-09-01 00:00:00
abstract：:The first description of endothelial progenitor cells (EPC) in 1997 led rapidly to substantial changes in our understanding of angiogenesis, and within 5 years to the first clinical studies in humans using bone marrow derived EPC to enhance coronary neovascularisation and cardiac function after myocardial ischemia. Ho...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2009.12.004
更新日期：2010-05-01 00:00:00
abstract：:Studies of microvascular geometry made from microscope observations of tissues in vivo or after perfusion with a silastic elastomer or india ink are restricted to a two-dimensional field of view. Microvascular corrosion casts, however, if of sufficient rigidity and structural integrity, can yield three-dimensional inf...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90044-4
更新日期：1983-01-01 00:00:00
abstract：:Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1029
更新日期：1994-05-01 00:00:00
abstract：:A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green)...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.11.012
更新日期：2011-03-01 00:00:00
abstract：:We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title：Microvascular research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.01.004
更新日期：2010-03-01 00:00:00
abstract：:Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2010.09.005
更新日期：2010-12-01 00:00:00
abstract：:Recently the zebrafish has emerged as a promising vertebrate model of lymphatic vasculature development. The establishment of numerous transgenic lines that label the lymphatic endothelium in the zebrafish has allowed the fine examination of the developmental timing and the anatomy of their lymphatic vasculature. Alth...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2014.06.001
更新日期：2014-11-01 00:00:00
abstract：:A cytochemical study of vascular and cellular development in fetal adipose tissue was conducted utilizing 10 plant lectins (fluorescein isothiocyanate (FITC)-labeled), antibodies against laminin, types II and IV collagen, and a probe for actin. Throughout fetal development (50-110 days) blood vessels were stained by g...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90012-z
更新日期：1991-01-01 00:00:00
abstract：:Tetrahydroxystilbene glucoside (TSG) is the main water-soluble component in Polygonum multiflorum Thunb, and it has many cardioprotective effects. Although TSG is able to relax blood vessels, its relaxation of rat superior mesenteric arteries and the underlying mechanism of this process are not clearly understood. The...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.10.007
更新日期：2019-05-01 00:00:00
abstract：:Overexpression of EGFR plays a key-role in head and neck squamous cell carcinoma (HNSCC) and justifies the extensive use of cetuximab, a monoclonal anti-EGFR antibody, as well as EGFR-tyrosine kinase inhibitors (EGFR-TKI), which have been reported to inhibit tumor cell growth and the secretion of pro-angiogenic factor...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.07.008
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Impairments at the microvascular level might lead to more overt cardiovascular complications, therefore, being able to early detect microvascular dysfunction would be beneficial. Thus, the present study investigated whether near-infrared spectroscopy (NIRS) assessment of microvascular responsiveness (reoxyge...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.04.009
更新日期：2019-09-01 00:00:00
abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2043
更新日期：1997-11-01 00:00:00
abstract：:Metabolic syndrome (MetS) is a combination of clinical disorders that together increase the risk for cardiovascular disease and diabetes. SHRSP.Z-Lepr(fa)/IzmDmcr (SHRSP.ZF) rats with MetS show impaired nitric oxide-mediated relaxation in coronary and mesenteric arteries, and angiotensin II receptor type 1 blockers pr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.04.001
更新日期：2013-07-01 00:00:00
