Abstract:
:Studies of microvascular geometry made from microscope observations of tissues in vivo or after perfusion with a silastic elastomer or india ink are restricted to a two-dimensional field of view. Microvascular corrosion casts, however, if of sufficient rigidity and structural integrity, can yield three-dimensional information when examined under the scanning electron microscope. We have used modified Batson's No. 17 anatomical casting compound (having a shrinkage less than 1% on setting) to prepare casts of the microvasculature of the heart and skeletal muscles in anesthetized rats. In casts from the L. ventricle the capillary network appeared to parallel the arrangement of the muscle fibers, but showed many capillary loops and anastomoses. In skeletal muscles (gastrocnemius and gracilis) held at full extension, in situ, the casts showed long straight capillaries with fewer branchings than in the heart. In shortened skeletal muscle the capillaries exhibited an undulatory configuration. Capillary diameters (mean +/- SD) were 5.14 +/- 1.42 micrometers (N = 202), 5.04 +/- 1.45 micrometers (N = 294) and 4.84 +/- 1.97 micrometers (N = 335) in L. ventricle, gastrocnemius, and gracilis muscles (both shortened), respectively. The mean values for capillary diameter in these three tissues did not differ significantly. Combining our data with those of L. Henquell, P. L. LaCelle, and C. R. Honig on erythrocyte deformability in the rat (Microvasc. Res. 12, 259-274 (1976)) suggests that even when the capillary bed is fully distended the smallest capillaries, amounting to 1-2% of the total number, must be channels for plasma flow alone. In cross-sectional views of the casts from contracted skeletal muscle the capillaries appeared to form a tightly meshed network of convoluted vessels around the fibers, such that in some regions a large fraction of the surface of each fiber was in contact with blood. The Krogh cylinder geometry appears not to be appropriate for modeling O2 transport in maximally shortened skeletal muscle; a more appropriate model may be that of a cylindrical muscle fiber supplied, at any point down its length, by a uniform peripheral O2 supply.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Potter RF,Groom ACdoi
10.1016/0026-2862(83)90044-4subject
Has Abstractpub_date
1983-01-01 00:00:00pages
68-84issue
1eissn
0026-2862issn
1095-9319journal_volume
25pub_type
杂志文章abstract:BACKGROUND:Vascular hyporeactivity in severe hemorrhagic shock could induce refractory hypotension and is an important cause of death. The global acute inflammatory response induced in shock triggers the over-expression of reactive oxygen species, NO, ET1 and TNF-α, which play essential roles in the pathology of vascul...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.12.006
更新日期:2016-05-01 00:00:00
abstract::Most forms of tissue healing depend critically on revascularisation. In soft tissues and in vitro, mechanical stimuli have been shown to promote vessel-forming activity. However, in bone defects, increased interfragmentary motion impairs vascular regeneration. Because these effects seem contradictory, we aimed to dete...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.08.002
更新日期:2009-12-01 00:00:00
abstract::Patients with diabetic polyneuropathy are known to have an impaired neurovascular reflex arc compared to healthy controls. This is seen in a delayed decrease in microcirculation of the ipsilateral hand after cooling of the contralateral hand. The aim of this pilot study was to investigate whether intravenous alpha-lip...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2151
更新日期:1999-07-01 00:00:00
abstract::An increasing body of evidence suggests that the deleterious effects of Acute Kidney Injury (AKI) on remote organ function could, at least in part, be due to loss of the normal balance of immune, inflammatory, and soluble mediator metabolism that attends injury of the tubular epithelium. Such dysregulation, acting at ...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2008.09.001
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:To examine the possible role of Klotho (Kl) in human microvasculature. METHODS:The expression level of Kl in primary human dermal microvascular endothelial cells (HDMECs) and primary human dermal fibroblasts (HFb) was detected by real-time polymerase chain reaction amplification (qRT-PCR), Western blot analyse...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2016.05.005
更新日期:2016-09-01 00:00:00
abstract::Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.03.001
更新日期:2010-07-01 00:00:00
abstract::The large secreted glycoprotein thrombospondin-1 is a potent inhibitor of neovascularization in vivo. In order to better understand its mechanism of action, we have determined the full range of deficits thrombospondin can impose on cultured capillary endothelial cells. Exogenously added thrombospondin-1 blocked the ab...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1997.2015
更新日期:1997-07-01 00:00:00
abstract::We describe a sensitive, reliable, and convenient procedure to assay cell adhesion based on flow cytometry which allows quantitative studies of all major peripheral blood leukocytes adhering to microvascular endothelial cells. Using this assay, we show that adhesion of mononuclear cells is significantly increased in t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1996.0047
更新日期:1996-09-01 00:00:00
abstract::We studied the absorbing peripheral lymphatic vessel with the light microscope, the transmission electron microscope, the scanning electron microscope, and three-dimensional models of the diaphragm of several rodents and insectivores under normal and experimental conditions (lymphatic stasis and dehydration). To clari...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2101
更新日期:1999-01-01 00:00:00
abstract::Routine pulmonary ultrasound for diagnosis of disease or injury relies on interpretation of image features, such as comet-tail artifacts, which can also be indicative of the poorly understood phenomenon of ultrasound-induced pulmonary capillary hemorrhage (PCH). Evans blue extraction and bronchoalveolar lavage (BAL) w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::Cardiovascular diseases, among all diseases, are taking the most victims worldwide. Coronary artery occlusion, takes responsibility of about 30% of the yearly global deaths in the world (Heart Disease and Stroke Statistics 2017 At-a-Glance, 2017), raising the need for viable substitutes for cardiovascular tissues. Dep...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2020.104027
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:The proepicardium (PE), a transient embryonic structure crucial for the development of the epicardium and heart, contains its own population of endothelial cells (ECs). The aim of our study was to determine the pattern, anatomical orientation and phenotypic marker expression of the endothelial cell network w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.08.007
更新日期:2015-11-01 00:00:00
abstract::We tested the hypothesis that the endothelial nitric oxide (NO)-soluble guanylyl cyclase system is involved in atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) mediated regulation of coronary and peripheral vascular resistance. Rat hearts were perfused via the aorta at constant flow and the effect...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2289
更新日期:2001-01-01 00:00:00
abstract::The role of VEGF during development and in pathology is well known, but its function in normal adult tissues is poorly understood. Adverse effects associated with the use of anti-angiogenic therapies targeting VEGF in human pathologies have begun to reveal potential functions of VEGF in quiescent vasculature. Further ...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2007.03.004
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVE:Hypertension and hypercholesterolemia elicit inflammatory and thrombogenic responses in the microvasculature. However, little is known about whether and how risk factor combinations alter microvascular function. We examined how the actions of HTN+HCh on the microvasculature differ from the responses elicited ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2016.01.002
更新日期:2016-05-01 00:00:00
abstract::We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2356
更新日期:2001-11-01 00:00:00
abstract::Current tissue engineering techniques have failed to provide an established microvasculature in skin substitutes, a requisite for the maintenance of graft viability and rapid revascularization subsequent to graft transplantation in vivo. To improve outcomes for both conventional skin grafts and skin substitutes, the e...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2007.04.012
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:Vascular endothelial cells (ECs) are constantly exposed to blood flow associated forces such as cyclic strain due to blood pressure, which affects ECs survival and angiogenesis by producing ROS via NAD(P)H oxidase. NAD(P)H oxidase subunit p22phox is reported to be related to the development of atherosclerosis...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.08.001
更新日期:2009-03-01 00:00:00
abstract::The effects of systemic injections of vasoactive substances were studied in the micro-circulation of the hamster skin fold window preparation, which can be observed without anesthesia, exposure, and acute surgical procedures. The effects were characterized by the continuous measurement of the diameter of the arterial ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90014-1
更新日期:1984-09-01 00:00:00
abstract::Immunological studies of human endothelial cells have been performed almost entirely on endothelial cells (EC) derived from foetal (umbilical vein) or adult (aorta) large vessels. Immunologically these EC have in common the fact that they are negative for major histocompatibility complex (MHC) class II antigens (HLA-D...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1996.0016
更新日期:1996-03-01 00:00:00
abstract::The multiple pore model of T. R. Harris and R. J. Roselli (1981, J. Appl. Physiol: Respir. Environ. Exercise Physiol. 50, 1-14), was used to simulate lung lymph flow and protein transport at various levels of microvascular pressure. Response of the three-pore structure determined in that study was found to be in excel...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90022-6
更新日期:1985-05-01 00:00:00
abstract::Injection of labeled microspheres is an established method in animal models to analyze the capillary organ blood flow at different time points. However, the microspheres can lead to stenoses of the capillary lumen, which might affect tissue oxygen supply. Our study aimed to investigate the influence of repeated inject...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.04.001
更新日期:2011-07-01 00:00:00
abstract::Nonspecific vasodilatation during iontophoresis is an important confounding factor in experimental pharmacology. In this investigation, we studied the involvement of sensory nerves and histamine-related reactions in causing nonspecific vasodilatation in a model of anodal and cathodal iontophoresis of sodium chloride. ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2003.12.002
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Tissue ischemia and reperfusion (I/R) affects blood flow restoration and oxygen delivery to the damaged tissues contributing to tissue morbidity and microcirculatory compromise. Pulsed acoustic cellular expression (PACE) technology is known to support tissue neovascularization. The aim of this study was to t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.11.005
更新日期:2012-03-01 00:00:00
abstract::A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90058-1
更新日期:1988-03-01 00:00:00
abstract::Particle adhesion in vivo is highly dependent on the microvascular environment comprising of unique anatomical, geometrical, physiological fluid flow conditions and cell-particle and cell-cell interactions. Hence, proper design of vascular-targeted drug carriers that efficiently deliver therapeutics to the targeted ce...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.02.004
更新日期:2015-05-01 00:00:00
abstract::We observed the retinal capillary pericytes of the rat in situ. Whole retinae were mounted, immediately post vivo, in a special tissue chamber for electronic light microscopy at high magnifications. Under electronic light microscopy the pericytes could be clearly distinguished from the endothelial cells. In addition, ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2086
更新日期:1998-07-01 00:00:00
abstract::Since the internal carotid artery supplies blood to both the eye and the brain, ocular microvascular hemodynamics can be altered due to ischemic stroke. The purpose of the current study was to establish the feasibility of conjunctival microcirculation imaging for detection of inter-ocular differences in microvascular ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.04.007
更新日期:2015-07-01 00:00:00
abstract::Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly when fluorescent visu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90022-2
更新日期:1988-11-01 00:00:00
abstract::The steatotic liver is characterized by deranged intrahepatic microvasculature that is believed to predispose it to ischemia-reperfusion injury. The aim of this study was to investigate the distorted hepatic hemodynamics and its impact on the redox status of the steatotic liver. Hepatic hemodynamic parameters, hepatic...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00021-9
更新日期:2003-07-01 00:00:00