Abstract：
:Overexpression of EGFR plays a key-role in head and neck squamous cell carcinoma (HNSCC) and justifies the extensive use of cetuximab, a monoclonal anti-EGFR antibody, as well as EGFR-tyrosine kinase inhibitors (EGFR-TKI), which have been reported to inhibit tumor cell growth and the secretion of pro-angiogenic factors by tumor cells, such as VEGF and IL-8. Moreover, vessel normalization in tumors, suggesting a more complex mediation of endothelial cell growth control has also been observed in vivo. The present study was designed to investigate the angiogenic consequences of exposure of HNSCC tumor cell lines to cetuximab and intercellular signaling between tumor and endothelial cells by secretion of pro- and anti-angiogenic mediators in the conditioned media (CM). The results achieved showed that cetuximab decreased the secretion of VEGF by HNSCC cells and that exposure of human umbilical vein endothelial cells (HUVEC) to CM from HNSCC cells exposed to cetuximab induced an increase in endothelial cell network formation. Angiogenesis proteome profiling showed that cetuximab induced a complex alteration of the secretion of pro- and anti-angiogenic factors by HNSCC cells without enabling to identify a unique molecular marker. Expression of endothelial membrane receptors (VEGFR-2, EGFR, PECAM-1 and Notch-4) was investigated and only EGFR expression was found influenced when HUVEC were exposed to CM from cetuximab-exposed HNSCC cells. These results showed that the decrease in the secretion of pro-angiogenic agents like VEGF by HNSCC cells exposed to cetuximab could not be sufficient to justify its anti-angiogenic activity in vitro.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Jouan-Hureaux V,Boura C,Merlin JL,Faivre Bdoi
10.1016/j.mvr.2011.07.008subject
Has Abstractpub_date
2012-03-01 00:00:00pages
131-7issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(11)00123-3journal_volume
83pub_type
杂志文章abstract：:A theoretical model, appropriate for data interpretation, is presented for the transcapillary fluid exchange and associated intraluminal hydrodynamic and solute transport occurring in the microocclusion of single capillaries. This analysis describes the spatial and temporal behavior of the intraluminal flow. A compari...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90012-2
更新日期：1983-03-01 00:00:00
abstract：:Microvascular blood flow is commonly calculated from a "centerline" velocity derived from the dual-slit method. This is done by relating the computed centerline velocity to an average velocity through an empirically derived conversion factor. To determine the physical basis of the conversion factor, we utilized a Four...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90046-0
更新日期：1989-05-01 00:00:00
abstract：:This paper proposes a mathematical model for vessel recruitment in the microvascular coronary network. The model is based on microvascular network units (MVNUs), where we define a MVNU as a portion of the microvascular network comprising seven generations of identical, parallel-arranged vessels (upstream arteries, lar...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.11.006
更新日期：2016-03-01 00:00:00
abstract：:Concentration-response curves are often used as indicators of vascular reactivity and receptor sensitivity. Some investigators use ethylenediaminetetraacetate (EDTA) or ascorbic acid (ASC) to prevent catecholamine oxidation but many others do not. This difference may affect estimates of reactivity. We tested the effec...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90057-2
更新日期：1983-07-01 00:00:00
abstract：:Alterations in the reactivity of arterioles to vasodilator agonists were assessed in the skeletal muscle microcirculation of age-matched, normotensive male Sprague-Dawley rats fed either a high salt (4% NaCl, HS) or a low salt (0.4% NaCl, LS) diet and in reduced renal mass hypertensive rats on a high salt diet (HSRRM)...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2105
更新日期：1998-11-01 00:00:00
abstract：:Laser Doppler flowmetry (LDF) of forearm skin blood flow, combined with iontophoretically-administered acetylcholine and sodium nitroprusside and wavelet spectral analysis, was used for noninvasive evaluation of endothelial function in 17 patients newly diagnosed with New York Heart Association class II-III congestive...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.07.006
更新日期：2008-11-01 00:00:00
abstract：:Recent studies indicate that the human choroid has a considerable capacity to keep blood flow constant despite exercise-induced increases in perfusion pressure. The mechanisms underlying this vasoconstrictor response remain unclear. We hypothesized that pharmacological modulation of the autonomic nervous system may al...
journal_title：Microvascular research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0026-2862(02)00010-9
更新日期：2003-01-01 00:00:00
abstract：:An increasing body of evidence suggests that the deleterious effects of Acute Kidney Injury (AKI) on remote organ function could, at least in part, be due to loss of the normal balance of immune, inflammatory, and soluble mediator metabolism that attends injury of the tubular epithelium. Such dysregulation, acting at ...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2008.09.001
更新日期：2009-01-01 00:00:00
abstract：OBJECTIVE:Hypertension and hypercholesterolemia elicit inflammatory and thrombogenic responses in the microvasculature. However, little is known about whether and how risk factor combinations alter microvascular function. We examined how the actions of HTN+HCh on the microvasculature differ from the responses elicited ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.01.002
更新日期：2016-05-01 00:00:00
abstract：:Shear stress causes release of nitric oxide (NO) from microvascular endothelial cells in vivo and stimulates their growth in vitro. After chronic electrical stimulation of lower hind limb skeletal muscles in the rat, measurements of capillary diameters and red blood cell velocity indicated that shear stress is increas...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2193
更新日期：2000-01-01 00:00:00
abstract：:Capillary diameters in sartorius muscle of frogs were measured in vivo by means of a new computer video method, based on the passage of red blood cells (RBCs) through the capillary (C. Ellis, R. Sanfranyos, and A. Groom (1983), Microvasc. Res. 26, 139-150). The distribution of capillary diameters from 21 frogs was rep...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90066-3
更新日期：1983-09-01 00:00:00
abstract：:Studies of microvascular geometry made from microscope observations of tissues in vivo or after perfusion with a silastic elastomer or india ink are restricted to a two-dimensional field of view. Microvascular corrosion casts, however, if of sufficient rigidity and structural integrity, can yield three-dimensional inf...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90044-4
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:Tissue ischemia and reperfusion (I/R) affects blood flow restoration and oxygen delivery to the damaged tissues contributing to tissue morbidity and microcirculatory compromise. Pulsed acoustic cellular expression (PACE) technology is known to support tissue neovascularization. The aim of this study was to t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.11.005
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Sepsis is a fatal systemic inflammatory response syndrome caused by severe infection. The aim of this study was to measure hepatic microcirculation during the sepsis with laser speckle contrast imaging (LSCI), as well as investigating the underlying mechanisms. METHODS:Sepsis was induced by cecal ligation a...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.10.006
更新日期：2015-01-01 00:00:00
abstract：:Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.11.002
更新日期：2014-01-01 00:00:00
abstract：:Thrombolysis treatment of acute ischemic stroke is limited by the pro-edematous and hemorrhagic effects exerted by reperfusion, which disrupts the blood-brain barrier (BBB) capillary endothelium in the infarct core. Most studies of the ischemic BBB overlook the complexity of the penumbral area, where the affected brai...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.12.002
更新日期：2014-01-01 00:00:00
abstract：:Pericytes are known to communicate with endothelial cells by direct contact and by releasing cytokines such as TGF-beta. There is also strong evidence that pericytes act as regulators of endothelial cell proliferation and differentiation. We have investigated the effect of pericyte-conditioned medium (PCM) on prolifer...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2208
更新日期：2000-01-01 00:00:00
abstract：:Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2010.09.005
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.10.008
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:Studies on the acute effects of coffee on the microcirculation have shown contradicting results. This study aimed to investigate if intake of caffeine-containing coffee changes blood flow and microvascular reactivity in the skin. METHODS:We measured acute changes in cutaneous vascular conductance (CVC) in th...
journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2017.06.006
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVES:Calcium channel inhibitors have beneficial impact on microcirculation, but beta-blocker effect is controversial. Clinicians still do not agree on beta-blocker combination with other treatments in the management of impaired microcirculation. The aim of the present study was to describe the effects of beta-blo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.04.004
更新日期：2011-07-01 00:00:00
abstract：:The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(02)00035-3
更新日期：2003-03-01 00:00:00
abstract：:A simple model has been developed for simulation of oxygen transport to and from blood flowing in conduits of the diameter of arterioles and larger (greater than or equal to 20 microns). The basis is the large capillary model (LCAP) of P.K. Nair, et al. 1989 which has been validated experimentally. Detailed calculatio...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90070-8
更新日期：1990-03-01 00:00:00
abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：:An intravital microscopic method for quantitative measurement of interstitial concentrations of fluorescent tracers has been applied to the investigation of microvascular permeability in the hamster cheek pouch. Some nanoliters of FITC-dextran mean mol wt (Mw) 20,000, 3000, or sodium fluorescein (Mw 376) were injected...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(86)90009-9
更新日期：1986-01-01 00:00:00
abstract：:The relative magnitude of vascular reactivity to inhaled gas stimuli in the major retinal arterioles has not been systematically investigated. The purpose of this study was to compare the magnitude of retinal vascular reactivity in response to inhaled gas provocation at equivalent measurement sites in the superior-, a...
journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2011.11.002
更新日期：2012-03-01 00:00:00
abstract：:An ex vivo perfused solid tumor preparation provides control over the physiological, biochemical, and pharmacological composition of the arterial input and easy access to the venous output. This is advantageous for studies of transport and metabolism in solid tumors. Here we present a tissue-isolated tumor preparation...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1063
更新日期：1994-11-01 00:00:00
abstract：:Histone acetylation/deacetylation is a key mechanism for regulating transcription, which plays an important role in the control of gene expression, tissue growth, and development. In particular, histone deacetylase 7 (HDAC7), a member of class IIa HDACs, is crucial in maintaining vascular integrity. Endothelial progen...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.10.006
更新日期：2014-01-01 00:00:00
abstract：:The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90045-1
更新日期：1984-01-01 00:00:00
abstract：:We have developed an intensity analysis technique for fluorescence microscopy that allows us to measure, in real time, the diameter and the membrane potential or intracellular calcium ([Ca(2+)]i) of in vivo arteriolar endothelium or smooth muscle. Cheek pouch arterioles of anesthetized hamsters were luminally or ablum...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2315
更新日期：2001-07-01 00:00:00
