Abstract:
BACKGROUND:Sepsis is a fatal systemic inflammatory response syndrome caused by severe infection. The aim of this study was to measure hepatic microcirculation during the sepsis with laser speckle contrast imaging (LSCI), as well as investigating the underlying mechanisms. METHODS:Sepsis was induced by cecal ligation and puncture. Rats were divided into the sham group and sepsis group. The hepatic microcirculation was monitored with LSCI. In addition, hepatic endothelial function (expression of cell adhesion molecules, coagulation and vascular permeability) and neutrophils accumulation in the liver were compared between the two groups. RESULTS:During the sepsis, hepatic microcirculation decreased dramatically (290.3±70.1 LSPU (laser speckle perfusion units) at baseline vs. 230.4±60.7 LSPU at 12h vs. 125.2±25.4 LSPU at 48h, P<0.001). The rats developed hyperbilirubinemia since 6h. In the early phase of sepsis, the accumulation of neutrophils and formation of microthrombus increased rapidly. In the late phase, hepatic neutrophils accumulation was already at its maximum level. Meanwhile, the endothelial coagulation status shifted from procoagulation to anticoagulation. The vascular leakage was involved in the microcirculation dysfunction since 12h after sepsis. CONCLUSIONS:Hepatic microcirculation dysfunction occurs early during the sepsis and is associated with liver injury. This microcirculation dysfunction is due to neutrophil-endothelium interactions, microthrombus formation and vascular leakage.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Wu Y,Ren J,Zhou B,Ding C,Chen J,Wang G,Gu G,Liu S,Li Jdoi
10.1016/j.mvr.2014.10.006subject
Has Abstractpub_date
2015-01-01 00:00:00pages
137-46eissn
0026-2862issn
1095-9319pii
S0026-2862(14)00164-2journal_volume
97pub_type
杂志文章abstract::Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title:Microvascular research
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更新日期:2021-01-01 00:00:00
abstract::We have performed a mathematical simulation on a CYBER computer of the release, diffusion, and consumption of oxygen in the capillaries and surrounding tissue of peripheral nerve under steady-state conditions. The Krogh-Erlang equation was used to calculate oxygen tension in tissue, while numerical solution of the dif...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(87)90054-9
更新日期:1987-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,评审
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更新日期:2013-05-01 00:00:00
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journal_title:Microvascular research
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doi:10.1016/s0026-2862(03)00021-9
更新日期:2003-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2151
更新日期:1999-07-01 00:00:00
abstract::We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...
journal_title:Microvascular research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.mvr.2010.01.002
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.008
更新日期:2018-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90051-2
更新日期:1985-09-01 00:00:00
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2341
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2017.12.001
更新日期:2018-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90012-2
更新日期:1983-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90044-4
更新日期:1983-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2101
更新日期:1999-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.08.007
更新日期:2015-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2010.10.001
更新日期:2011-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mvr.2017.06.006
更新日期:2017-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90030-x
更新日期:1984-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2018.03.012
更新日期:2018-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.01.007
更新日期:2015-03-01 00:00:00
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journal_title:Microvascular research
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doi:10.1006/mvre.2002.2437
更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90018-3
更新日期:1983-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0026-2862(84)90060-8
更新日期:1984-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.03.004
更新日期:2004-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.11.001
更新日期:2005-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(89)90023-x
更新日期:1989-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1012
更新日期:1994-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.04.001
更新日期:2011-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2279
更新日期:2000-11-01 00:00:00