当前位置： SCI文献检索 > MICROVASCULAR RESEARCH期刊下所有文献 > Hypoxia and vascular endothelial growth factor acutely up-regulate angiopoietin-1 and Tie2 mRNA in bovine retinal pericytes.

Hypoxia and vascular endothelial growth factor acutely up-regulate angiopoietin-1 and Tie2 mRNA in bovine retinal pericytes.

Abstract：

:The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/Tie2 system has been studied, but these studies are limited mostly to endothelial cells. In this study, using bovine retinal pericytes (BRP), we investigated the effect of two known angiogenic stimuli, hypoxia and vascular endothelial growth factor (VEGF) treatment, on the regulation of the angiopoietin/Tie2 system. Hypoxia (2% O(2) concentration) was acquired by a hypoxia chamber. Both hypoxia and VEGF (10 ng/ml) treatment significantly increased angiopoietin-1 (Ang1) mRNA expression. This marked augmentation occurred acutely (maximal increase at 2 h) and subsequently decreased. In contrast, angiopoietin-2 (Ang2) mRNA expression was unaltered in BRP upon both treatment. Significant up-regulation of Tie2 mRNA expression was found and lasted up to 12 h. However, using bovine aortic endothelial cells (BAEC), we found that only Ang2 expression, but neither Ang1 nor Tie2, responded to these two angiogenic stimuli, which was consistent with many previous reports. In conclusion, our data suggest that both hypoxia and VEGF treatment differentially regulate the angiopoietin/Tie2 system in the two vascular cells and that, particularly in BRP, the regulation of Ang1, but not Ang2, and Tie2 expression may play an important role in vascular development.

journal_name

Microvasc Res

journal_title

Microvascular research

authors

Park YS,Kim NH,Jo I

doi

10.1016/s0026-2862(02)00035-3

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

125-31

issue

2

eissn

0026-2862

issn

1095-9319

pii

S0026286202000353

journal_volume

65

pub_type

杂志文章

相关文献

MICROVASCULAR RESEARCH文献大全
  • Variation in red blood cell deformability and possible consequences for oxygen transport to tissue.

    abstract：:Red blood cell (RBC)-plasma suspensions with a hematocrit of 10-12% were forced to flow through microchannels (equivalent diameter 6 microns, equivalent length 20 microns, 2600 in parallel) by applying a pressure difference of 20 cm H2O. Transit times for 100 microliters of fresh suspensions from 33 healthy male subje...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1994.1017

    authors： Kikuchi Y,Da QW,Fujino T

    更新日期：1994-03-01 00:00:00

  • Angiogenin in middle-aged type 1 diabetes patients.

    abstract：BACKGROUND:Angiogenin levels are increased in children and adolescent patients with type 1 diabetes, regardless of the extent of diabetic microangiopathy. However, little is known about the angiogenin concentrations in adults with type 1 diabetes. Thus we studied its level in middle aged subjects with the presence of d...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2012.08.005

    authors： Neubauer-Geryk J,Kozera GM,Wolnik B,Szczyrba S,Nyka WM,Bieniaszewski L

    更新日期：2012-11-01 00:00:00

  • Expression and regulation of tumor necrosis factor-α-induced protein-8-like 2 is associated with acute lung injury induced by myocardial ischemia reperfusion in diabetic rats.

    abstract：AIMS:The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. METHODS:Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR); diabetes+sham (D +...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2020.104009

    authors： Kong Q,Yuan M,Ming T,Fang Q,Wu X,Song X

    更新日期：2020-07-01 00:00:00

  • Microscopic visualization of the retina by angiography with high-molecular-weight fluorescein-labeled dextrans in the mouse.

    abstract：:Methods currently available for the examination of the retinal vasculature of laboratory animals have significant drawbacks. Fluorescein angiography of rodent eyes is hampered by a poor view of the peripheral retina and difficulty in performing fundus photography. Methods of staining or filling retinal vessels are unr...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1993.1042

    authors： D'Amato R,Wesolowski E,Smith LE

    更新日期：1993-09-01 00:00:00

  • Sunitinib effects on the radiation response of endothelial and breast tumor cells.

    abstract：BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2013.10.008

    authors： El Kaffas A,Al-Mahrouki A,Tran WT,Giles A,Czarnota GJ

    更新日期：2014-03-01 00:00:00

  • A mathematical model for the vessel recruitment in coronary microcirculation in the absence of active autoregulation.

    abstract：:This paper proposes a mathematical model for vessel recruitment in the microvascular coronary network. The model is based on microvascular network units (MVNUs), where we define a MVNU as a portion of the microvascular network comprising seven generations of identical, parallel-arranged vessels (upstream arteries, lar...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2015.11.006

    authors： Saracco A,Bauckneht M,Verna E,Ghiringhelli S,Repetto R,Sambuceti G,Provasoli S,Storace M

    更新日期：2016-03-01 00:00:00

  • Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel.

    abstract：:A hallmark of severe inflammation is reactive oxygen species (ROS) overproduction induced by increased inflammatory mediators secretion. During systemic inflammation, inflammation mediators circulating in the bloodstream interact with endothelial cells (ECs) raising intracellular oxidative stress at the endothelial mo...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2014.02.001

    authors： Sarmiento D,Montorfano I,Cerda O,Cáceres M,Becerra A,Cabello-Verrugio C,Elorza AA,Riedel C,Tapia P,Velásquez LA,Varela D,Simon F

    更新日期：2015-03-01 00:00:00

  • Mechanisms which mediate the antiapoptotic effects of angiopoietin-1 on endothelial cells.

    abstract：:The main objective of this study was to identify molecular mechanisms through which angiopoietin-1 (Ang-1), a ligand for Tie-2 receptors, influences endothelial cell apoptosis. Human umbilical vein endothelial cells were cultured in a medium enriched with 2% fetal bovine serum (FBS) and growth supplements. Apoptosis w...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.2002.2421

    authors： Harfouche R,Hasséssian HM,Guo Y,Faivre V,Srikant CB,Yancopoulos GD,Hussain SN

    更新日期：2002-07-01 00:00:00

  • Inhibition of Rho kinase decreases hydraulic and protein microvascular permeability in cat skeletal muscle.

    abstract：:Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/s0026-2862(03)00041-4

    authors： Lundblad C,Bentzer P,Grände PO

    更新日期：2003-09-01 00:00:00

  • Expression of adhesion molecules by cultured human glomerular endothelial cells in response to cytokines: comparison to human umbilical vein and dermal microvascular endothelial cells.

    abstract：:We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.2001.2356

    authors： Murakami S,Morioka T,Nakagawa Y,Suzuki Y,Arakawa M,Oite T

    更新日期：2001-11-01 00:00:00

  • Comparison between pore model predictions and sheep lung fluid and protein transport.

    abstract：:The multiple pore model of T. R. Harris and R. J. Roselli (1981, J. Appl. Physiol: Respir. Environ. Exercise Physiol. 50, 1-14), was used to simulate lung lymph flow and protein transport at various levels of microvascular pressure. Response of the three-pore structure determined in that study was found to be in excel...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(85)90022-6

    authors： Roselli RJ,Parker RE,Harris TR

    更新日期：1985-05-01 00:00:00

  • Alpha adrenoceptor agonist-induced microcirculatory oscillations are reduced in diabetic neuropathy.

    abstract：:In diabetic patients small fiber neuropathy has been associated with impairment of 0.1 Hz microvascular vasomotion. The aim of this study was (1) to investigate whether vasoconstriction-induced microvascular oscillations in the skin are reduced in diabetic patients with peripheral and/or autonomic neuropathy, and (2) ...

    journal_title：Microvascular research

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.mvr.2008.04.004

    authors： Schmiedel O,Nurmikko TJ,Schroeter ML,Whitaker R,Harvey JN

    更新日期：2008-08-01 00:00:00

  • Oxidative stress in response to high glucose levels in endothelial cells and in endothelial progenitor cells: evidence for differential glutathione peroxidase-1 expression.

    abstract：:Endothelial cells and endothelial progenitor cells (EPCs) play a key role in the pathogenesis of vascular disease. Both cell types are affected by the oxidative stress but their susceptibility may be different. This study aimed to investigate the antioxidative enzymes activated in EPCs after high constant glucose expo...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2010.05.004

    authors： Felice F,Lucchesi D,di Stefano R,Barsotti MC,Storti E,Penno G,Balbarini A,Del Prato S,Pucci L

    更新日期：2010-12-01 00:00:00

  • 7-Ketocholesterol activates caspases-3/7, -8, and -12 in human microvascular endothelial cells in vitro.

    abstract：:7-Ketocholesterol (7kCh) is a major oxysterol found associated with vascular diseases. Human microvascular endothelial cells (HMVECs) were cultured with different concentrations of 7kCh with and without inhibitors. Cell viabilities and caspase activities were assessed. 7kCh caused loss of cell viability in a dose-depe...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2007.10.003

    authors： Luthra S,Dong J,Gramajo AL,Chwa M,Kim DW,Neekhra A,Kuppermann BD,Kenney MC

    更新日期：2008-04-01 00:00:00

  • Hemorrhagic shock and nitric oxide release from erythrocytic nitric oxide synthase: a quantitative analysis.

    abstract：:A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2009.02.009

    authors： Chen K,Pittman RN,Popel AS

    更新日期：2009-06-01 00:00:00

  • Skin blood flow from gas transport: helium xenon and laser Doppler compared.

    abstract：:A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(88)90058-1

    authors： Neufeld GR,Galante SR,Whang JM,DeVries D,Baumgardner JE,Graves DJ,Quinn JA

    更新日期：1988-03-01 00:00:00

  • Topical application of cerium nitrate prevents burn edema after burn plasma transfer.

    abstract：:Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2009.07.006

    authors： Kremer T,Hernekamp F,Riedel K,Peter Ch,Gebhardt MM,Germann G,Heitmann Ch,Walther A

    更新日期：2009-12-01 00:00:00

  • Voltage-gated potassium channels and NOS contribute to a sustained cutaneous vasodilation elicited by local heating in an interactive manner in young adults.

    abstract：:Local skin heating to 42°C causes rapid increases in cutaneous perfusion (initial peak), followed by a brief nadir and subsequent sustained elevation (plateau). Several studies have demonstrated that nitric oxide synthase (NOS) largely contributes to the plateau response during local heating. In this study, we tested ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2017.12.001

    authors： Fujii N,Halili L,Nishiyasu T,Kenny GP

    更新日期：2018-05-01 00:00:00

  • A simple model for prediction of oxygen transport rates by flowing blood in large capillaries.

    abstract：:A simple model has been developed for simulation of oxygen transport to and from blood flowing in conduits of the diameter of arterioles and larger (greater than or equal to 20 microns). The basis is the large capillary model (LCAP) of P.K. Nair, et al. 1989 which has been validated experimentally. Detailed calculatio...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(90)90070-8

    authors： Nair PK,Huang NS,Hellums JD,Olson JS

    更新日期：1990-03-01 00:00:00

  • Use of tadalafil in a patient with a secondary Raynaud's phenomenon not responding to sildenafil.

    abstract：:The Raynaud's phenomenon often accompanies systemic rheumatic diseases and is also known as a vascular side effect of chemotherapy. Therapy of the Raynaud's phenomenon with nitrates or calcium-channel-blockers is rarely beneficial. In contrast, the PDE-V-inhibitor sildenafil seems to be effective in these patients. Fo...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2005.03.001

    authors： Baumhaekel M,Scheffler P,Boehm M

    更新日期：2005-05-01 00:00:00

  • A novel assay of angiogenesis in the quail chorioallantoic membrane: stimulation by bFGF and inhibition by angiostatin according to fractal dimension and grid intersection.

    abstract：:In a novel assay of angiogenesis in the quail chorioallantoic membrane (CAM), we measured vascular pattern and angiogenic rate after homogeneous exposure of the entire vascular tree to recognized modulators of vessel growth. In comparison to phosphate-buffered saline (PBS)-treated controls, the vascular stimulator, ba...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1998.2073

    authors： Parsons-Wingerter P,Lwai B,Yang MC,Elliott KE,Milaninia A,Redlitz A,Clark JI,Sage EH

    更新日期：1998-05-01 00:00:00

  • Estrogen induces endothelial progenitor cells proliferation and migration by estrogen receptors and PI3K-dependent pathways.

    abstract：:Estrogen induces endothelial progenitor cells (EPCs) migration and proliferation, which may serve as a potential target for coronary artery disease, but the mechanisms are unclear. We hypothesized that estrogen receptors (ERs) and phosphatidylinositol 3-kinase (PI3K) signaling pathway, which represent particularly imp...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2007.02.009

    authors： Zhao X,Huang L,Yin Y,Fang Y,Zhao J,Chen J

    更新日期：2008-01-01 00:00:00

  • Pigment epithelium-derived factor inhibits leptin-induced angiogenesis by suppressing vascular endothelial growth factor gene expression through anti-oxidative properties.

    abstract：:Leptin, a circulating hormone secreted mainly from adipose tissues, is involved in the control of body weight. Recently, leptin was found to be an angiogenic factor, and its vitreous levels are associated with angiogenic eye diseases such as proliferative diabetic retinopathy. However, the molecular mechanism for lept...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/s0026-2862(03)00005-0

    authors： Yamagishi S,Amano S,Inagaki Y,Okamoto T,Takeuchi M,Inoue H

    更新日期：2003-05-01 00:00:00

  • Cyclic strain disrupts endothelial network formation on Matrigel.

    abstract：:Most forms of tissue healing depend critically on revascularisation. In soft tissues and in vitro, mechanical stimuli have been shown to promote vessel-forming activity. However, in bone defects, increased interfragmentary motion impairs vascular regeneration. Because these effects seem contradictory, we aimed to dete...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2009.08.002

    authors： Wilson CJ,Kasper G,Schütz MA,Duda GN

    更新日期：2009-12-01 00:00:00

  • Quantitative analysis of the microvasculature growing in the fibrin interface between a skin graft and the recipient site.

    abstract：:Current tissue engineering techniques have failed to provide an established microvasculature in skin substitutes, a requisite for the maintenance of graft viability and rapid revascularization subsequent to graft transplantation in vivo. To improve outcomes for both conventional skin grafts and skin substitutes, the e...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2007.04.012

    authors： Wu X,Kathuria N,Patrick CW,Reece GP

    更新日期：2008-01-01 00:00:00

  • Endothelial progenitor cells--an evolving story.

    abstract：:The first description of endothelial progenitor cells (EPC) in 1997 led rapidly to substantial changes in our understanding of angiogenesis, and within 5 years to the first clinical studies in humans using bone marrow derived EPC to enhance coronary neovascularisation and cardiac function after myocardial ischemia. Ho...

    journal_title：Microvascular research

    pub_type： 杂志文章,评审

    doi：10.1016/j.mvr.2009.12.004

    authors： Pearson JD

    更新日期：2010-05-01 00:00:00

  • Lumen formation and other angiogenic activities of cultured capillary endothelial cells are inhibited by thrombospondin-1.

    abstract：:The large secreted glycoprotein thrombospondin-1 is a potent inhibitor of neovascularization in vivo. In order to better understand its mechanism of action, we have determined the full range of deficits thrombospondin can impose on cultured capillary endothelial cells. Exogenously added thrombospondin-1 blocked the ab...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1997.2015

    authors： Tolsma SS,Stack MS,Bouck N

    更新日期：1997-07-01 00:00:00

  • Chronic elevations in salt intake and reduced renal mass hypertension compromise mechanisms of arteriolar dilation.

    abstract：:Alterations in the reactivity of arterioles to vasodilator agonists were assessed in the skeletal muscle microcirculation of age-matched, normotensive male Sprague-Dawley rats fed either a high salt (4% NaCl, HS) or a low salt (0.4% NaCl, LS) diet and in reduced renal mass hypertensive rats on a high salt diet (HSRRM)...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1998.2105

    authors： Frisbee JC,Lombard JH

    更新日期：1998-11-01 00:00:00

  • Evidence of bidirectional flow in the sciatic vasa nervorum.

    abstract：:The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2014.05.008

    authors： Olver DT,Lacefield JC,Shoemaker KJ

    更新日期：2014-07-01 00:00:00

  • Effects of ascorbic acid and EDTA on vascular concentration-response to catecholamines.

    abstract：:Concentration-response curves are often used as indicators of vascular reactivity and receptor sensitivity. Some investigators use ethylenediaminetetraacetate (EDTA) or ascorbic acid (ASC) to prevent catecholamine oxidation but many others do not. This difference may affect estimates of reactivity. We tested the effec...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(83)90057-2

    authors： Maxwell LC,Herlihy JT,Riedel GL

    更新日期：1983-07-01 00:00:00