Abstract：
:A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green) as well as Ulex europaeus lectin, visualized in green fluorescence. Further, we present possibilities for quantitative evaluation of the capillary network which implies the length of capillaries per unit volume of muscle tissue (Lcap/Vmuscle) and the length of capillaries supplying individual muscle fibres per unit fibre length (Lcap/Lfib), per surface area (Lcap/Sfib) and per volume (Lcap/Vfib) as well as the course of capillaries in the muscle. The latter can be described by the tortuosity, orientation and mean capillary length. To get reasonable results we met the following requirements: i) high quality thick tissue sections, from which 3D image data were acquired; ii) immunofluorescent methods suitable for confocal microscopy; iii) penetration of the fluorescent dyes throughout the tissue section; iv) proper 3D image analysis methods for performing reliable measurements and v) control over relevant tissue deformations. The developed methodology is illustrated by results obtained from autopsy or biopsy samples of three human muscles, i.e. vastus lateralis, multifidus and masseter muscle that exhibit differences in genetic background, innervation, tasks and functional activity.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Janáček J,Cvetko E,Kubínová L,Travnik L,Eržen Idoi
10.1016/j.mvr.2010.11.012subject
Has Abstractpub_date
2011-03-01 00:00:00pages
231-8issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(10)00236-0journal_volume
81pub_type
杂志文章abstract：:The role of VEGF during development and in pathology is well known, but its function in normal adult tissues is poorly understood. Adverse effects associated with the use of anti-angiogenic therapies targeting VEGF in human pathologies have begun to reveal potential functions of VEGF in quiescent vasculature. Further ...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2007.03.004
更新日期：2007-09-01 00:00:00
abstract：:The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90060-8
更新日期：1984-05-01 00:00:00
abstract：:Nonspecific vasodilatation during iontophoresis is an important confounding factor in experimental pharmacology. In this investigation, we studied the involvement of sensory nerves and histamine-related reactions in causing nonspecific vasodilatation in a model of anodal and cathodal iontophoresis of sodium chloride. ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2003.12.002
更新日期：2004-03-01 00:00:00
abstract：:Red blood cell (RBC)-plasma suspensions with a hematocrit of 10-12% were forced to flow through microchannels (equivalent diameter 6 microns, equivalent length 20 microns, 2600 in parallel) by applying a pressure difference of 20 cm H2O. Transit times for 100 microliters of fresh suspensions from 33 healthy male subje...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1017
更新日期：1994-03-01 00:00:00
abstract：:Using the laser-Doppler method we measured blood flow on the nailfold skin to compare the reactivity of cutaneous microcirculation in three groups of normotensive subjects: 11 subjects with a familial predisposition to hypertension without a previous record of high blood pressure, 6 predisposed subjects with a previou...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2279
更新日期：2000-11-01 00:00:00
abstract：:The steatotic liver is characterized by deranged intrahepatic microvasculature that is believed to predispose it to ischemia-reperfusion injury. The aim of this study was to investigate the distorted hepatic hemodynamics and its impact on the redox status of the steatotic liver. Hepatic hemodynamic parameters, hepatic...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00021-9
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND:The proepicardium (PE), a transient embryonic structure crucial for the development of the epicardium and heart, contains its own population of endothelial cells (ECs). The aim of our study was to determine the pattern, anatomical orientation and phenotypic marker expression of the endothelial cell network w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.08.007
更新日期：2015-11-01 00:00:00
abstract：:This study determined the contribution of cytochrome P450 (CP450) 4A enzyme metabolites of arachidonic acid in mediating the constriction of isolated rat skeletal muscle resistance arteries in response to elevated PO2. Gracilis arteries (GA) were viewed via television microscopy and constrictor responses to elevated P...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2341
更新日期：2001-11-01 00:00:00
abstract：:Focal adhesion kinase (FAK) is a key component of cell-substratum adhesions, known as focal adhesion complexes. Growing evidence indicates that FAK is important in maintenance of normal cell survival and that disruption of FAK signaling results in loss of substrate adhesion and anoikis (apoptosis) of anchorage-depende...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2011.05.003
更新日期：2012-01-01 00:00:00
abstract：:We observed the retinal capillary pericytes of the rat in situ. Whole retinae were mounted, immediately post vivo, in a special tissue chamber for electronic light microscopy at high magnifications. Under electronic light microscopy the pericytes could be clearly distinguished from the endothelial cells. In addition, ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2086
更新日期：1998-07-01 00:00:00
abstract：:Matrix Gla Protein (MGP) is an ECM molecule commonly associated with dysfunctions of large blood vessels such as arteriosclerosis and atherosclerosis. However, the exact role of MGP in the microvasculature is not clear. Utilizing a mouse MGP knockout model we found that MGP suppresses angiogenic sprouting from mouse a...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.10.005
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Angiogenin levels are increased in children and adolescent patients with type 1 diabetes, regardless of the extent of diabetic microangiopathy. However, little is known about the angiogenin concentrations in adults with type 1 diabetes. Thus we studied its level in middle aged subjects with the presence of d...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.08.005
更新日期：2012-11-01 00:00:00
abstract：:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the pres...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1064
更新日期：1995-11-01 00:00:00
abstract：:We examined the impact of lymph flow obstruction in large post-nodal lymphatic vessels in sheep. A silk ligature was placed 2 cm downstream from the prescapular or popliteal lymph node and tightened to interrupt flow. At 6, 12 and 16 weeks after lymph flow blockage, a network of small interconnecting lymphatics (appro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.11.003
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:Vascular endothelial cells (ECs) are constantly exposed to blood flow associated forces such as cyclic strain due to blood pressure, which affects ECs survival and angiogenesis by producing ROS via NAD(P)H oxidase. NAD(P)H oxidase subunit p22phox is reported to be related to the development of atherosclerosis...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.08.001
更新日期：2009-03-01 00:00:00
abstract：:The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90040-8
更新日期：1985-07-01 00:00:00
abstract：:Visualization of the lymphatic system is clinically necessary during diagnosis or treatment of many conditions and diseases; it is used for identifying and monitoring lymphedema, for detecting metastatic lesions during cancer staging and for locating lymphatic structures so they can be spared during surgical procedure...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2014.06.006
更新日期：2014-11-01 00:00:00
abstract：:In a novel assay of angiogenesis in the quail chorioallantoic membrane (CAM), we measured vascular pattern and angiogenic rate after homogeneous exposure of the entire vascular tree to recognized modulators of vessel growth. In comparison to phosphate-buffered saline (PBS)-treated controls, the vascular stimulator, ba...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2073
更新日期：1998-05-01 00:00:00
abstract：OBJECTIVE:In the present study, we used a two-kidney-two-clip (2k2c) stroke-prone renovascular hypertension rat model (RHRSP) to investigate the protective effects of ligustrazine (TMP) on cerebral arteries and to examine PI3K/Akt pathway behavior under this protection. METHODS:The cerebral artery remodeling was induc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103938
更新日期：2020-03-01 00:00:00
abstract：:Reduced lung capillary expression of angiotensin I-converting enzyme (ACE), a key enzyme in cardiovascular pathophysiology, and of caveolin-1, an important regulator of endothelial cell signalling, has been demonstrated in various models of pulmonary arterial hypertension (PAH). We addressed the relationship between P...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.04.008
更新日期：2010-09-01 00:00:00
abstract：:Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.003
更新日期：2012-09-01 00:00:00
abstract：:Strychnine is known to possess anti-inflammatory and antitumour activity, but its roles in tumour angiogenesis, the key step involved in tumour growth and metastasis, and the involved molecular mechanism are still unknown. We aimed to investigate the effects of strychnine on key components of inflammatory angiogenesis...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.01.003
更新日期：2013-05-01 00:00:00
abstract：:Microalbuminuria is a primary manifestation of diabetic nephropathy. Endothelial cell-selective adhesion molecule (ESAM) is a new member of the immunoglobulin superfamily which is selectively expressed by vascular endothelial cells. Although ESAM mediates homophilic interaction between endothelial cells, the role of E...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.01.002
更新日期：2009-05-01 00:00:00
abstract：:The microcirculation in normal and arthritic juxtaarticular bone was studied in 16 young dogs with carragheenan-induced arthritis of one knee. The regional blood flow was determined by the tissue uptake of intracardially injected 15-microns 141Ce-labeled microspheres, and the microvascular plasma volume was determined...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90020-r
更新日期：1990-09-01 00:00:00
abstract：:The dynamics of the microvasculature of the blood-perfused canine gastrocnemius-plantaris muscle in situ at rest and during contraction were determined using multiple-indicator dilution analysis. Permeability-surface area product (PS) was estimated using a bolus indicator dilution of 86Rb, with 125I-albumin serving as...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2067
更新日期：1998-03-01 00:00:00
abstract：:Although microvascular dysfunction (MVD) has been well characterized in individual organs as different disease entities, clinical evidence is mounting in support of an underlying systemic process. To address this hypothesis, we systematically searched PubMed and Medline for studies in adults published between 2014 and...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2020.104123
更新日期：2021-03-01 00:00:00
abstract：:Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.011
更新日期：2012-11-01 00:00:00
abstract：:The objective of this study was to provide the first quantitative measurements of the process of vascular growth in the rat peritoneal disseminated tumor model in both arteriolar and venular systems. Male Fischer 344 rats were injected intraperitoneally with 1 x 10(7) cells of colon carcinoma cell line (RCN-9). Three ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0003
更新日期：1996-01-01 00:00:00
abstract：:Hypoxia-inducible factor-1 (HIF-1) orchestrates angiogenesis under hypoxic conditions mainly due to increased expression of such target genes as vascular endothelial growth factor (VEGF). Na+/H+exchanger-1 (NHE1), a potential HIF target gene product, plays a pivotal role in proliferation, survival, migration, adhesion...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.12.004
更新日期：2011-03-01 00:00:00
abstract：:The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90089-8
更新日期：1992-11-01 00:00:00
