Expression of adhesion molecules in cultured human pulmonary microvascular endothelial cells.


:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the presence of factor VIII-related antigen, incorporation of DiI-Ac-LDL, tubule-like structure formation in Matrigel, and positive staining for ACE. Adhesion molecules were tested at passage 3 and passage 12. Cells demonstrated intense staining for PECAM-1 both unstimulated and stimulated with TNF-alpha (20 ng/ml). The adhesion molecules ICAM-1, VCAM-1, ELAM-1, and P-selectin differed in expression on unstimulated cells. ICAM-1 was constitutively expressed on unstimulated cells and the expression was increased by TNF-alpha stimulation (20 hr). In contrast, VCAM-1, ELAM-1, and P-selectin were not detected on unstimulated cells but were detected after stimulation with TNF-alpha. The inducibility of adhesion molecules was different. VCAM-1 (10 hr) and ELAM-1 (4 hr) were expressed more strongly than P-selectin (minutes to 4 hr). The adhesion molecule profile found on passage 12 was the same as on passage 3. CD36 was not detected on both unstimulated and stimulated (4 and 8 hr) cells. The peak of adhesion of HL-60 cells to TNF-alpha activated HPMEC monolayers was around 8 hr. The results indicate that HPMEC can be continuously grown in vitro for many passages without losing their adhesion molecule expression. This expression of adhesion molecules confirms that HPMECs might be a good in vitro model in the understanding of various aspects of pulmonary microvascular endothelial cell function and may be useful as the basis for studies of adhesion molecule targeted therapies of pulmonary inflammatory diseases.


Microvasc Res


Microvascular research


Shen J,Ham RG,Karmiol S




Has Abstract


1995-11-01 00:00:00














  • Altered regulator mechanisms of presynaptic adrenergic nerve: a new physiopathological hypothesis in Raynaud's disease.

    abstract::The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Giovanni B,Giuseppina CM,Susanna F,Roberto M

    更新日期:1984-01-01 00:00:00

  • VEGF-PKD1-HDAC7 signaling promotes endothelial progenitor cell migration and tube formation.

    abstract::Histone acetylation/deacetylation is a key mechanism for regulating transcription, which plays an important role in the control of gene expression, tissue growth, and development. In particular, histone deacetylase 7 (HDAC7), a member of class IIa HDACs, is crucial in maintaining vascular integrity. Endothelial progen...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Yu D,Chen W,Ren J,Zhang T,Yang K,Wu G,Liu H

    更新日期:2014-01-01 00:00:00

  • Chronic elevations in salt intake and reduced renal mass hypertension compromise mechanisms of arteriolar dilation.

    abstract::Alterations in the reactivity of arterioles to vasodilator agonists were assessed in the skeletal muscle microcirculation of age-matched, normotensive male Sprague-Dawley rats fed either a high salt (4% NaCl, HS) or a low salt (0.4% NaCl, LS) diet and in reduced renal mass hypertensive rats on a high salt diet (HSRRM)...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Frisbee JC,Lombard JH

    更新日期:1998-11-01 00:00:00

  • Improved conjunctival microcirculation in diabetic retinopathy patients with MTHFR polymorphisms after Ocufolin™ Administration.

    abstract:PURPOSE:To investigate conjunctival microvascular responses in patients with mild diabetic retinopathy (MDR) and methylenetetrahydrofolate reductase (MTHFR) polymorphisms (D + PM) after administration of Ocufolin™, a medical food containing 900 μg l-methylfolate (levomefolate calcium or [6S]-5-methyltetrahydrofolic aci...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Liu Z,Jiang H,Townsend JH,Wang J

    更新日期:2020-11-01 00:00:00

  • Angiogenesis and antiangiogenic therapy in endometriosis.

    abstract::Endometriosis, the presence of endometrium-like tissue outside of the uterine cavity, is a common disease among women of reproductive age. Typical symptoms include abdominal pain and painful menstruation. In addition, endometriosis is associated with reduced fertility. Current treatment modalities, the surgical remova...

    journal_title:Microvascular research

    pub_type: 杂志文章,评审


    authors: Becker CM,D'Amato RJ

    更新日期:2007-09-01 00:00:00

  • Regional differences in spontaneously occurring angiogenesis in the adult rat mesentery.

    abstract::Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Hansen-Smith FM,Joswiak GR,Baustert JL

    更新日期:1994-05-01 00:00:00

  • Sublingual glyceryl trinitrate and the peripheral thermal responses in normal and cold-sensitive individuals.

    abstract::Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Hope K,Eglin C,Golden F,Tipton M

    更新日期:2014-01-01 00:00:00

  • Low-frequency blood flow oscillations in congestive heart failure and after beta1-blockade treatment.

    abstract::Laser Doppler flowmetry (LDF) of forearm skin blood flow, combined with iontophoretically-administered acetylcholine and sodium nitroprusside and wavelet spectral analysis, was used for noninvasive evaluation of endothelial function in 17 patients newly diagnosed with New York Heart Association class II-III congestive...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Bernjak A,Clarkson PB,McClintock PV,Stefanovska A

    更新日期:2008-11-01 00:00:00

  • Endothelial progenitor cells from peripheral blood support bone regeneration by provoking an angiogenic response.

    abstract::Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of ne...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Goerke SM,Obermeyer J,Plaha J,Stark GB,Finkenzeller G

    更新日期:2015-03-01 00:00:00

  • Polyarginine and eosinophil-derived major basic protein increase microvascular permeability independently of histamine or nitric oxide release.

    abstract::We tested the hypothesis that cationic peptides (polyarginine; poly-argn) and eosinophil-derived major basic protein (MaBP) increase permeability by stimulating the release of histamine and/or nitric oxide. We used intravital microscopy, clearance, and integrated optical intensity (IOI), using FITC-dextran 150 (FITC-d...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Minnicozzi M,Ramírez MM,Egan RW,Gleich GJ,Kobayashi I,Kim D,Durán WN

    更新日期:1995-07-01 00:00:00

  • Skin microcirculatory effect of exogenous calcitonin gene-related peptide (CGRP) evaluated by laser Doppler flowmetry coupled with iontophoresis in healthy subjects.

    abstract::The aim of our study was to evaluate the feasibility of laser Doppler flowmetry (LDF) coupled with iontophoresis in exploring the skin vasodilator activity of exogenous calcitonin gene-related peptide (CGRP) in healthy subjects and to investigate the mechanisms involved in the skin vasodilator activity of this peptide...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Rossi M,Carpi A,Di Maria C,Galetta F,Santoro G

    更新日期:2007-03-01 00:00:00

  • Evidence of bidirectional flow in the sciatic vasa nervorum.

    abstract::The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Olver DT,Lacefield JC,Shoemaker KJ

    更新日期:2014-07-01 00:00:00

  • The Rho-kinase inhibitor Y-27632 inhibits cholestasis-induced platelet interactions in the hepatic microcirculation.

    abstract::Bile duct obstruction is associated with hepatic accumulation of leukocytes and liver injury. Emerging data suggest that platelets may play an important role in tissue damage and inflammation. Herein, we characterized the platelet response in cholestatic liver injury and evaluated the role of Rho-kinase signaling. For...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Laschke MW,Dold S,Menger MD,Jeppsson B,Thorlacius H

    更新日期:2009-06-01 00:00:00

  • Heterogeneity of capillary diameters in skeletal muscle of the frog.

    abstract::Capillary diameters in sartorius muscle of frogs were measured in vivo by means of a new computer video method, based on the passage of red blood cells (RBCs) through the capillary (C. Ellis, R. Sanfranyos, and A. Groom (1983), Microvasc. Res. 26, 139-150). The distribution of capillary diameters from 21 frogs was rep...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Safranyos RG,Ellis CG,Tyml K,Groom AC

    更新日期:1983-09-01 00:00:00

  • Pigment epithelium-derived factor inhibits oxidative stress-induced apoptosis and dysfunction of cultured retinal pericytes.

    abstract::Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis in the mammalian eye, suggesting that loss of PEDF is implicated in the pathogenesis of proliferative diabetic retinopathy. However, a role for PEDF in early diabetic retinopathy remains to be elucidated. Since oxidative stress is thought t...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Amano S,Yamagishi S,Inagaki Y,Nakamura K,Takeuchi M,Inoue H,Imaizumi T

    更新日期:2005-01-01 00:00:00

  • Effect of dispersion of vessel diameters and lengths in stochastic networks. II. Modeling of microvascular hematocrit distribution.

    abstract::A microvascular network model described in the preceding paper (B. Dawant, M. Levin, and A. S. Popel, 1986, Microvasc. Res. 31, 203-222) featuring random distribution of vessel diameters and lengths, is extended to calculate the distributions of red blood cell flux and discharge hematocrit throughout the network. A re...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Levin M,Dawant B,Popel AS

    更新日期:1986-03-01 00:00:00

  • Skin blood flow from gas transport: helium xenon and laser Doppler compared.

    abstract::A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Neufeld GR,Galante SR,Whang JM,DeVries D,Baumgardner JE,Graves DJ,Quinn JA

    更新日期:1988-03-01 00:00:00

  • Capillary diameter and geometry in cardiac and skeletal muscle studied by means of corrosion casts.

    abstract::Studies of microvascular geometry made from microscope observations of tissues in vivo or after perfusion with a silastic elastomer or india ink are restricted to a two-dimensional field of view. Microvascular corrosion casts, however, if of sufficient rigidity and structural integrity, can yield three-dimensional inf...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Potter RF,Groom AC

    更新日期:1983-01-01 00:00:00

  • Modification of vascular and inflammation biomarkers after OGTT in overweight healthy and diabetic subjects.

    abstract::We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...

    journal_title:Microvascular research

    pub_type: 杂志文章,多中心研究


    authors: Derosa G,D'Angelo A,Salvadeo SA,Ferrari I,Fogari E,Gravina A,Mereu R,Palumbo I,Maffioli P,Randazzo S,Cicero AF

    更新日期:2010-03-01 00:00:00

  • Spontaneous cyclic contractions of the capillary wall in vivo, impeding red cell flow: a quantitative analysis. Evidence for endothelial contractility.

    abstract::We have videorecorded spontaneous cyclic contractions of capillary walls which often stopped the flow of blood cells, in spleens of rat and mouse. An inverted microscope and oblique lighting were key elements in obtaining images in which the boundaries of cells composing vessel walls were clearly distinguishable. Usin...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Ragan DM,Schmidt EE,MacDonald IC,Groom AC

    更新日期:1988-07-01 00:00:00

  • The quantitative development of glomerular capillaries in rats with special reference to unbiased stereological estimates of their number and sizes.

    abstract::The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Nyengaard JR

    更新日期:1993-05-01 00:00:00

  • Vascular architecture in free flaps: Analysis of vessel morphology and morphometry in murine free flaps.

    abstract::The aim of this study was to analyze the development of vascular architecture as well as vascular morphometry and morphology of anastomosed microvascular free flaps. Free pectoral skin flaps were raised in 25 rats and anastomosed to the femoral vessels in the groin region. CD31 immunohistology was performed after 3, 7...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Pabst AM,Kämmerer PW,Krüger M,Jäger L,Ackermann M

    更新日期:2018-07-01 00:00:00

  • A mathematical model for the vessel recruitment in coronary microcirculation in the absence of active autoregulation.

    abstract::This paper proposes a mathematical model for vessel recruitment in the microvascular coronary network. The model is based on microvascular network units (MVNUs), where we define a MVNU as a portion of the microvascular network comprising seven generations of identical, parallel-arranged vessels (upstream arteries, lar...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Saracco A,Bauckneht M,Verna E,Ghiringhelli S,Repetto R,Sambuceti G,Provasoli S,Storace M

    更新日期:2016-03-01 00:00:00

  • Effects of endotoxin on lung pericytes in vitro.

    abstract::Lipopolysaccharide released during bacterial sepsis causes acute lung injury and ARDS. Pulmonary microvascular injury is a feature of ARDS, and vascular remodeling develops, leading to pulmonary hypertension. Pericytes in the lung circulation proliferate and contribute to the remodeling seen in experimental sepsis. It...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Khoury J,Langleben D

    更新日期:1998-09-01 00:00:00

  • Arginase inhibition enhances angiogenesis in endothelial cells exposed to hypoxia.

    abstract::Hypoxia-induced arginase elevation plays an essential role in several vascular diseases but influence of arginase on hypoxia-mediated angiogenesis is completely unknown. In this study, in vitro network formation in bovine aortic endothelial cells (BAEC) was examined after exposure to hypoxia for 24h with or without ar...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Wang L,Bhatta A,Toque HA,Rojas M,Yao L,Xu Z,Patel C,Caldwell RB,Caldwell RW

    更新日期:2015-03-01 00:00:00

  • Inhibition of capillary growth in chronically stimulated rat muscles by N(G)-nitro-l-arginine, nitric oxide synthase inhibitor.

    abstract::Shear stress causes release of nitric oxide (NO) from microvascular endothelial cells in vivo and stimulates their growth in vitro. After chronic electrical stimulation of lower hind limb skeletal muscles in the rat, measurements of capillary diameters and red blood cell velocity indicated that shear stress is increas...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Hudlická O,Brown MD,Silgram H

    更新日期:2000-01-01 00:00:00

  • Impaired function of cerebral parenchymal arterioles in experimental preeclampsia.

    abstract::Preeclampsia (PE), a dangerous hypertensive complication of pregnancy, is associated with widespread maternal vascular dysfunction. However, the effect of PE on the cerebral vasculature that can lead to stroke and cognitive decline is not well understood. We hypothesized that function of cortical parenchymal arteriole...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Johnson AC,Cipolla MJ

    更新日期:2018-09-01 00:00:00

  • The regional differences in the contribution of nitric oxide synthase to skin blood flow at forearm and lower leg sites in response to local skin warming.

    abstract::We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Del Pozzi AT,Carter SJ,Collins AB,Hodges GJ

    更新日期:2013-11-01 00:00:00

  • Quantitative differences in neoglycoprotein binding for vascular endothelial cells from porcine brain, ovary, and testis in vitro.

    abstract::Carrier-immobilized carbohydrates are valuable tools for assessing the glycoligand-binding capacity of cell surfaces. A panel of 10 types of fluorescent neoglycoproteins has been synthesized to determine the extent of their specific binding to endothelial cells in vitro that have been obtained from porcine brain, ovar...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Plendl J,Sinowatz F,Auerbach R,Gabius HJ

    更新日期:1995-09-01 00:00:00

  • Hypoxia and vascular endothelial growth factor acutely up-regulate angiopoietin-1 and Tie2 mRNA in bovine retinal pericytes.

    abstract::The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...

    journal_title:Microvascular research

    pub_type: 杂志文章


    authors: Park YS,Kim NH,Jo I

    更新日期:2003-03-01 00:00:00