Abstract:
:In preeclampsia, invasion of intrauterine decidual blood vessels by placental cytotrophoblasts is significantly reduced. This study examined the secretion of matrix metalloproteinases (MMP) by cultured human decidual endothelial cells from normal (NDEC) and preeclamptic (PEDEC) pregnancies. MMPs secreted into the culture medium were measured using zymography, Western blotting, and ELISA. Results were confirmed by Northern analysis. Phorbol myristate acetate, known to induce protease activity in other endothelial cell populations, stimulated MMP1, MMP9, and TIMP1 secretion in both NDEC and PEDEC. Neither tumor necrosis factor-alpha nor transforming growth factor-beta, both thought to have significant roles in the control of placentation, affected MMP secretion. MMP9 and TIMP1 levels were similar between the two cell types; however, MMP1 secretion was markedly different between the cell types. NDEC expressed higher levels of MMP1 under both basal (160 +/- 32 ng/10(6) cells) and stimulated (275 +/- 50) conditions compared to PEDEC (32 +/- 24 and 70 +/- 53, respectively). The lower MMP1 expression of decidual endothelial cells from preeclamptic women may inhibit endovascular invasion by cytotrophoblasts. These findings may, at least partly, explain the relative failure of trophoblasts to invade maternal decidual blood vessels in preeclamptic pregnancy.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Gallery ED,Campbell S,Arkell J,Nguyen M,Jackson CJdoi
10.1006/mvre.1998.2142keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
340-6issue
3eissn
0026-2862issn
1095-9319pii
S0026-2862(98)92142-2journal_volume
57pub_type
杂志文章abstract::Excessive generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS), by activated neutrophils and endothelial cells, has been implicated in the pathophysiology of endothelial barrier dysfunction. Disruption of the integrity of this barrier markedly increases permeability to fluids, solutes and in...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2008.10.005
更新日期:2009-01-01 00:00:00
abstract::Previous findings have shown that pulmonary fibroblasts are associated with preexisting holes in the endothelial and epithelial basal laminae through which neutrophils appear to enter and leave the interstitium as they migrate from capillaries to alveoli. To determine their role in neutrophil migration, fibroblast org...
journal_title:Microvascular research
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doi:10.1006/mvre.1996.0029
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abstract::Capillaries are often assumed to be indistensible. Only recently has the error caused by capillary distensibility in the measurement of hydraulic conductance been considered. An oil-drop method was used to measure simultaneously distensibility and hydraulic conductance (Lp) of mesenteric capillaries of 40 cranially pi...
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doi:10.1016/0026-2862(89)90013-7
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abstract::The steatotic liver is characterized by deranged intrahepatic microvasculature that is believed to predispose it to ischemia-reperfusion injury. The aim of this study was to investigate the distorted hepatic hemodynamics and its impact on the redox status of the steatotic liver. Hepatic hemodynamic parameters, hepatic...
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doi:10.1016/s0026-2862(03)00021-9
更新日期:2003-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2163
更新日期:1999-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2122
更新日期:1999-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1017
更新日期:1994-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2289
更新日期:2001-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.05.005
更新日期:2014-07-01 00:00:00
abstract::To compute two parameters of capillary protein transport of reflection coefficient (sigma) and permeability--surface area product (PS), two major methods have been employed. The first, or crosspoint method, uses two sets of lymph flow (Jv) and lymph/plasma protein concentration ratios (R) and solves the irreversible t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90051-2
更新日期:1985-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.10.005
更新日期:2013-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1028
更新日期:1994-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.12.001
更新日期:2015-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.001
更新日期:2015-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2086
更新日期:1998-07-01 00:00:00
abstract::We tested the hypothesis that p42/44MAPK and p38MAPK (mitogen-activated protein kinases; MAPK) signaling pathways regulate endothelial cell permeability to macromolecules. Passage 2-4 human umbilical vein endothelial cells (HUVEC) were grown to confluence on fibronectin-coated Snapwell membranes. The flux of fluoresce...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2381
更新日期:2002-03-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.08.001
更新日期:2009-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.mvr.2015.04.007
更新日期:2015-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 临床试验,杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.08.001
更新日期:2004-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2008.09.001
更新日期:2009-01-01 00:00:00
abstract::The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90045-1
更新日期:1984-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.03.001
更新日期:2010-07-01 00:00:00
abstract::The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1997.2043
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:Tissue ischemia and reperfusion (I/R) affects blood flow restoration and oxygen delivery to the damaged tissues contributing to tissue morbidity and microcirculatory compromise. Pulsed acoustic cellular expression (PACE) technology is known to support tissue neovascularization. The aim of this study was to t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2011.11.005
更新日期:2012-03-01 00:00:00
abstract::Hypoxia-induced arginase elevation plays an essential role in several vascular diseases but influence of arginase on hypoxia-mediated angiogenesis is completely unknown. In this study, in vitro network formation in bovine aortic endothelial cells (BAEC) was examined after exposure to hypoxia for 24h with or without ar...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.11.002
更新日期:2015-03-01 00:00:00