Abstract：
PURPOSE:Subcutaneous tissue is a promising site for cell transplantation; advantages include minimally invasive procedures and easy post-transplant monitoring. However, limited vascularity is the major known challenge. To address this challenge, a prevascularized graft bed is prepared in recipients. We aimed to establish an improved, clinically applicable approach to promote prevascularization of the subcutaneous graft bed prior to cell transplantation. METHODS:We applied a conventional prevascularization approach by subcutaneously implanting nylon discs into the backs of Lewis rats. After disc implantation, we treated rats with or without intermittent normobaric 100% oxygen inhalation (1 h, twice a day, for consecutive 7 days). We used histology to compare vascular density between the oxygen-treated or control groups. To assess the functional effects of prevascularization, we transplanted three hundred islets isolated from luciferase-transgenic Lewis rats into the oxygen-treated or control wild type Lewis recipients, then used bioluminescence imaging to track engraftment for 4 weeks. RESULTS:Oxygen treatment significantly augmented prevascularization in the subcutaneous site compared to controls. Islet transplantation into prevascularized graft beds demonstrated significant improvement in engraftment efficiency in oxygen-treated recipients compared to controls at 2-4 weeks post-transplantation. CONCLUSION:Combining intermittent normobaric 100% oxygen inhalation with a conventional vascularization approach promotes a functional vasculature within a week. A simple approach using normobaric oxygen has the potential for translation into clinical application in subcutaneous site cell transplantations.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Komatsu H,Gonzalez N,Kandeel F,Mullen Ydoi
10.1016/j.mvr.2020.104070subject
Has Abstractpub_date
2020-11-01 00:00:00pages
104070eissn
0026-2862issn
1095-9319pii
S0026-2862(20)30130-8journal_volume
132pub_type
杂志文章abstract：:A hallmark of severe inflammation is reactive oxygen species (ROS) overproduction induced by increased inflammatory mediators secretion. During systemic inflammation, inflammation mediators circulating in the bloodstream interact with endothelial cells (ECs) raising intracellular oxidative stress at the endothelial mo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.02.001
更新日期：2015-03-01 00:00:00
abstract：:The first description of endothelial progenitor cells (EPC) in 1997 led rapidly to substantial changes in our understanding of angiogenesis, and within 5 years to the first clinical studies in humans using bone marrow derived EPC to enhance coronary neovascularisation and cardiac function after myocardial ischemia. Ho...
journal_title：Microvascular research
pub_type： 杂志文章,评审
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更新日期：2010-05-01 00:00:00
abstract：:Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate th...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.10.003
更新日期：2019-05-01 00:00:00
abstract：:We have applied theories of mass transfer, in laminar and turbulent flow, to red cells in the stopped-flow apparatus in order to estimate the effect of extracellular diffusion boundary layers on the initial rate of O2 uptake. We compared the theoretical predictions with the results of our previous stopped-flow experim...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90058-4
更新日期：1983-07-01 00:00:00
abstract：:We determined whether the solvent drag reflection coefficient (sigma f) for total proteins of a canine perfused left lower lung lobe (LLL) preparation decreases at elevated venous pressures (Pv). We found that sigma f (estimated using the hematocrit-protein technique) remained constant at all Pv's (30-95 mm Hg) evalua...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90006-b
更新日期：1992-01-01 00:00:00
abstract：:The objective of this study was to provide the first quantitative measurements of the process of vascular growth in the rat peritoneal disseminated tumor model in both arteriolar and venular systems. Male Fischer 344 rats were injected intraperitoneally with 1 x 10(7) cells of colon carcinoma cell line (RCN-9). Three ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0003
更新日期：1996-01-01 00:00:00
abstract：:A simple model has been developed for simulation of oxygen transport to and from blood flowing in conduits of the diameter of arterioles and larger (greater than or equal to 20 microns). The basis is the large capillary model (LCAP) of P.K. Nair, et al. 1989 which has been validated experimentally. Detailed calculatio...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90070-8
更新日期：1990-03-01 00:00:00
abstract：:Methods currently available for the examination of the retinal vasculature of laboratory animals have significant drawbacks. Fluorescein angiography of rodent eyes is hampered by a poor view of the peripheral retina and difficulty in performing fundus photography. Methods of staining or filling retinal vessels are unr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1042
更新日期：1993-09-01 00:00:00
abstract：:The angiopoietin/Tie2 system is a predominant regulator of vascular development. This vascular development appears to be controlled and completed by the coordinated actions of two vascular cells, endothelial cells and their surrounding supporting cells, smooth muscle cells, or pericytes. The role of the angiopoietin/T...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(02)00035-3
更新日期：2003-03-01 00:00:00
abstract：:The large secreted glycoprotein thrombospondin-1 is a potent inhibitor of neovascularization in vivo. In order to better understand its mechanism of action, we have determined the full range of deficits thrombospondin can impose on cultured capillary endothelial cells. Exogenously added thrombospondin-1 blocked the ab...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2015
更新日期：1997-07-01 00:00:00
abstract：:The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1022
更新日期：1993-05-01 00:00:00
abstract：:The purpose of this study was to elucidate the mechanism by which bradykinin (BK) enhances prostacyclin (PGI(2)) production in human umbilical vein endothelial cells (HUVEC). BK-induced enhancement of PGI(2) synthesis was observed in a dose- and time-dependent manner, and it also increased [Ca(2+)](i) followed by enha...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2163
更新日期：1999-09-01 00:00:00
abstract：:The multiple pore model of T. R. Harris and R. J. Roselli (1981, J. Appl. Physiol: Respir. Environ. Exercise Physiol. 50, 1-14), was used to simulate lung lymph flow and protein transport at various levels of microvascular pressure. Response of the three-pore structure determined in that study was found to be in excel...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90022-6
更新日期：1985-05-01 00:00:00
abstract：:The Raynaud's phenomenon often accompanies systemic rheumatic diseases and is also known as a vascular side effect of chemotherapy. Therapy of the Raynaud's phenomenon with nitrates or calcium-channel-blockers is rarely beneficial. In contrast, the PDE-V-inhibitor sildenafil seems to be effective in these patients. Fo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.03.001
更新日期：2005-05-01 00:00:00
abstract：:Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00041-4
更新日期：2003-09-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2208
更新日期：2000-01-01 00:00:00
abstract：:This paper presents the results of a circulation analysis using an image based network model of a murine retinal vasculature, which closely represents the 3D vascular distribution of the retina. The uneven distribution of the red blood cells at vascular network bifurcations (i.e., plasma skimming effect), the microvas...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.02.005
更新日期：2010-07-01 00:00:00
abstract：:The aim of our study was to evaluate the feasibility of laser Doppler flowmetry (LDF) coupled with iontophoresis in exploring the skin vasodilator activity of exogenous calcitonin gene-related peptide (CGRP) in healthy subjects and to investigate the mechanisms involved in the skin vasodilator activity of this peptide...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.10.006
更新日期：2007-03-01 00:00:00
abstract：:Reperfusion of the ischemic lung is associated with increased pulmonary vascular resistance and reduced alveolar perfusion in conjunction with an inflammatory response. To determine the contribution of platelet-endothelial interactions, we examined effects of pulmonary ischemia-reperfusion (IR) on platelet adhesion an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2003.09.003
更新日期：2004-01-01 00:00:00
abstract：:The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.01.004
更新日期：2010-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
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更新日期：2003-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0026-2862(90)90032-m
更新日期：1990-11-01 00:00:00
abstract：:Nitrate supplementation in the form of beetroot juice has been shown to increase nitric oxide (NO) where nitrate can be reduced to nitrite and, subsequently, to NO through both nitric oxide synthase (NOS)-dependent and -independent pathways. We tested the hypothesis that nitrate supplementation would augment the NO co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.12.002
更新日期：2015-03-01 00:00:00
abstract：PURPOSE:To examine the possible role of Klotho (Kl) in human microvasculature. METHODS:The expression level of Kl in primary human dermal microvascular endothelial cells (HDMECs) and primary human dermal fibroblasts (HFb) was detected by real-time polymerase chain reaction amplification (qRT-PCR), Western blot analyse...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.05.005
更新日期：2016-09-01 00:00:00
abstract：:A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(88)90058-1
更新日期：1988-03-01 00:00:00
abstract：:We describe a sensitive, reliable, and convenient procedure to assay cell adhesion based on flow cytometry which allows quantitative studies of all major peripheral blood leukocytes adhering to microvascular endothelial cells. Using this assay, we show that adhesion of mononuclear cells is significantly increased in t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0047
更新日期：1996-09-01 00:00:00
abstract：:The contribution of the pleural mesothelium to pleural liquid and protein transport is still vigorously debated. Recent in vitro studies of stripped pleural membrane and free-standing pericardium have demonstrated active ion solute coupled transport of liquid and transcytosis of protein. However, the relative contribu...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.10.003
更新日期：2005-11-01 00:00:00
abstract：:Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.03.001
更新日期：2010-07-01 00:00:00
abstract：:Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.07.006
更新日期：2009-12-01 00:00:00
abstract：:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the pres...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1064
更新日期：1995-11-01 00:00:00
