Abstract：
:Hypoxia-induced arginase elevation plays an essential role in several vascular diseases but influence of arginase on hypoxia-mediated angiogenesis is completely unknown. In this study, in vitro network formation in bovine aortic endothelial cells (BAEC) was examined after exposure to hypoxia for 24h with or without arginase inhibition. Arginase activity, protein levels of the two arginase isoforms, eNOS, and VEGF as well as production of NO and ROS were examined to determine the involvement of arginase in hypoxia-mediated angiogenesis. Hypoxia elevated arginase activity and arginase 2 expression but reduced active p-eNOS(Ser1177) and NO levels in BAEC. In addition, both VEGF protein levels and endothelial elongation and network formation were reduced with continued hypoxia, whereas ROS levels increased and NO levels decreased. Arginase inhibition limited ROS, restored NO formation and VEGF expression, and prevented the reduction of angiogenesis. These results suggest a fundamental role of arginase activity in regulating angiogenic function.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Wang L,Bhatta A,Toque HA,Rojas M,Yao L,Xu Z,Patel C,Caldwell RB,Caldwell RWdoi
10.1016/j.mvr.2014.11.002subject
Has Abstractpub_date
2015-03-01 00:00:00pages
1-8eissn
0026-2862issn
1095-9319pii
S0026-2862(14)00170-8journal_volume
98pub_type
杂志文章abstract：:Failure of the glomerular barrier causing proteinuria has been modeled chiefly by Chang, Deen, and Brenner. They have refined models from an isoporous filter to a mostly isoporous membrane, which during proteinuric disease opens up nondiscriminating shunts. This report extends these concepts by measuring a larger dist...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2146
更新日期：1999-05-01 00:00:00
abstract：:Capillaries are often assumed to be indistensible. Only recently has the error caused by capillary distensibility in the measurement of hydraulic conductance been considered. An oil-drop method was used to measure simultaneously distensibility and hydraulic conductance (Lp) of mesenteric capillaries of 40 cranially pi...
journal_title：Microvascular research
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doi：10.1016/0026-2862(89)90013-7
更新日期：1989-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章,评审
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更新日期：2019-05-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90034-9
更新日期：1991-05-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.04.008
更新日期：2010-09-01 00:00:00
abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2043
更新日期：1997-11-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
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更新日期：1983-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.01.012
更新日期：2009-05-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.04.007
更新日期：2015-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2008.09.001
更新日期：2009-01-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.05.004
更新日期：2010-12-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.01.002
更新日期：2006-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103907
更新日期：2019-11-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.008
更新日期：2005-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2017.06.006
更新日期：2017-11-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.05.006
更新日期：2009-09-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.04.001
更新日期：2016-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90030-x
更新日期：1984-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.03.003
更新日期：2008-05-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.11.006
更新日期：2016-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.01.003
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pub_type： 杂志文章
doi：10.1006/mvre.1998.2105
更新日期：1998-11-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.10.004
更新日期：2016-01-01 00:00:00
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更新日期：1983-01-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.04.007
更新日期：2018-09-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.11.002
更新日期：2019-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0016
更新日期：1996-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.09.003
更新日期：2007-03-01 00:00:00
abstract：:Using the laser-Doppler method we measured blood flow on the nailfold skin to compare the reactivity of cutaneous microcirculation in three groups of normotensive subjects: 11 subjects with a familial predisposition to hypertension without a previous record of high blood pressure, 6 predisposed subjects with a previou...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2279
更新日期：2000-11-01 00:00:00
