Abstract：
:Lipopolysaccharide released during bacterial sepsis causes acute lung injury and ARDS. Pulmonary microvascular injury is a feature of ARDS, and vascular remodeling develops, leading to pulmonary hypertension. Pericytes in the lung circulation proliferate and contribute to the remodeling seen in experimental sepsis. It is unknown whether endotoxin can directly stimulate pericyte growth or induce contraction. We show that lipopolysaccharide from Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae increases rat lung pericyte proliferation in vitro by up to 72% on day 7 of exposure (P < 0.001), with E. coli being most potent. Lipid A is the active portion of the lipopolysaccharide, with equal activity at one-tenth the dose of lipopolysaccharide. Endotoxin's mitogenic effect requires the presence of serum, consistent with the requirement for a soluble CD14 receptor in the serum. Using FACS analysis, the pericytes lack surface CD14 receptors. Lipopolysaccharide exposure rapidly increases intracellular calcium and induces contraction of pericytes plated onto silicone membranes. Thus, endotoxin is a direct mitogen for lung pericytes in vitro and also induces pericyte contraction. Endotoxin, present in lung tissue early during sepsis, might directly contribute to the vascular remodeling in sepsis-induced lung injury.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Khoury J,Langleben Ddoi
10.1006/mvre.1998.2085subject
Has Abstractpub_date
1998-09-01 00:00:00pages
71-84issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(98)92085-4journal_volume
56pub_type
杂志文章abstract：:This study further investigates the mechanisms responsible for the effects of acute and severe hypoxia, and subsequent reoxygenation, on the contractility of isolated rat mesenteric arteries. In noradrenaline (NA)-contracted arteries, hypoxia caused a relaxation to near baseline levels. Reoxygenation resulted in an im...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.08.001
更新日期：2004-11-01 00:00:00
abstract：:Endometriosis, the presence of endometrium-like tissue outside of the uterine cavity, is a common disease among women of reproductive age. Typical symptoms include abdominal pain and painful menstruation. In addition, endometriosis is associated with reduced fertility. Current treatment modalities, the surgical remova...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2007.04.008
更新日期：2007-09-01 00:00:00
abstract：:Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104103
更新日期：2021-01-01 00:00:00
abstract：OBJECTIVE:Microvascular perfusion is indispensable for the growth and remodulation of membrane bone. Trauma, inflammation and surgical interventions may alter periosteal perfusion. However, there is not much known about periosteal perfusion in membrane bones. Therefore, the aim of this study was to establish a new chro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.09.008
更新日期：2009-03-01 00:00:00
abstract：BACKGROUND:Impairments at the microvascular level might lead to more overt cardiovascular complications, therefore, being able to early detect microvascular dysfunction would be beneficial. Thus, the present study investigated whether near-infrared spectroscopy (NIRS) assessment of microvascular responsiveness (reoxyge...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.04.009
更新日期：2019-09-01 00:00:00
abstract：:The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90089-8
更新日期：1992-11-01 00:00:00
abstract：:Electrophysiological characteristics of cultured human umbilical vein endothelial cells (HUVEC) were determined using the patch-clamp technique in the whole cell configuration. In isolated cells, membrane potential, capacitance, and input resistance were (Mean +/- SD) - 16.3 +/- 12.7 mV, 53.9 +/- 26 pF, and 2.3 +/- 1....
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1012
更新日期：1994-03-01 00:00:00
abstract：:The first description of endothelial progenitor cells (EPC) in 1997 led rapidly to substantial changes in our understanding of angiogenesis, and within 5 years to the first clinical studies in humans using bone marrow derived EPC to enhance coronary neovascularisation and cardiac function after myocardial ischemia. Ho...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2009.12.004
更新日期：2010-05-01 00:00:00
abstract：:Ischemia/Reperfusion injury and hemolysis are characterized by erythrocyte lysis and release of free heme into the microcirculation. Following substantial erythrocyte lysis, heme overwhelms circulatory heme-binding protein networks rapidly forming hemin, the oxidized form of iron protoporphyrin IX. Hemin's role in mod...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2007.01.002
更新日期：2007-05-01 00:00:00
abstract：:We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...
journal_title：Microvascular research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.mvr.2010.01.002
更新日期：2010-03-01 00:00:00
abstract：:The effect of hemodilution on oxygen delivery to the tissue was investigated analytically by taking into consideration the oxygen loss that occurs along the arterial microvasculature due to diffusion into the tissue and shunting to parallel running venules. The theoretical findings were related to experimental data on...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(87)90062-8
更新日期：1987-11-01 00:00:00
abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：:Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.11.002
更新日期：2014-01-01 00:00:00
abstract：:Histone acetylation/deacetylation is a key mechanism for regulating transcription, which plays an important role in the control of gene expression, tissue growth, and development. In particular, histone deacetylase 7 (HDAC7), a member of class IIa HDACs, is crucial in maintaining vascular integrity. Endothelial progen...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.10.006
更新日期：2014-01-01 00:00:00
abstract：:In a novel assay of angiogenesis in the quail chorioallantoic membrane (CAM), we measured vascular pattern and angiogenic rate after homogeneous exposure of the entire vascular tree to recognized modulators of vessel growth. In comparison to phosphate-buffered saline (PBS)-treated controls, the vascular stimulator, ba...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2073
更新日期：1998-05-01 00:00:00
abstract：:The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2236
更新日期：2000-07-01 00:00:00
abstract：:An increased recognition of the role of endothelial cells in disease and the development of methods for endothelial cell culture has led to an upsurge in in vitro studies of endothelial cell function. However, the cells most often used for these studies do not reflect the in vivo heterogeneity of endothelial cells. To...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2045
更新日期：1998-01-01 00:00:00
abstract：:The concept of anti-angiogenesis therapy was introduced by Judah Folkman in 1971 and since then, a plethora of pro- and anti-angiogenic factors have been identified. In the recent years, it has become clear that angiogenesis, the formation of new capillaries from a pre-existing capillary network, is highly regulated b...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2007.05.005
更新日期：2007-09-01 00:00:00
abstract：:Oxidative stress, which is defined as an imbalance between proxidant and antioxidant systems, is the essential mechanism involving in the ischemic process. During the early stage of brain ischemia, reactive oxygen species (ROS) are increased. Increased ROS are thought of a consequence of brain ischemia and exacerbatin...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.12.005
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:This study aims to investigate the correlation between the expression of miR-210 in peripheral blood and the number of peripheral endothelial progenitor cells (EPCs) in patients with type 2 diabetes mellitus (T2DM). We also determined the effect of miR-210 on EPC proliferation, adhesion, migration, tube forma...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104032
更新日期：2020-09-01 00:00:00
abstract：:Endoneurial capillaries in nerve biopsies from 12 adult diabetic cats with varying degrees of neurological dysfunction were examined for evidence of microvascular pathology and compared to nerves obtained at necropsy from 7 adult non-diabetic cats without clinical evidence of neurological dysfunction. As reported prev...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2007.12.002
更新日期：2008-04-01 00:00:00
abstract：:It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90008-f
更新日期：1990-07-01 00:00:00
abstract：:The purpose of the present study was to investigate the nervous control of gingival blood flow in cats. Gingival blood flow was measured by laser Doppler flowmeter in 75 cats during electrical stimulation and cutting or ligation of the inferior alveolar nerve and cervical sympathetic nerve without sympathectomy or pre...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90061-u
更新日期：1990-01-01 00:00:00
abstract：:This study aims to assess the relationship between spontaneous oscillations in changes in cerebral tissue oxyhemoglobin concentrations (Delta [HbO2]) and arterial blood pressure (ABP) signals in healthy elderly subjects during the resting state using wavelet coherence analysis. Continuous recordings of near-infrared s...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.02.008
更新日期：2014-05-01 00:00:00
abstract：:We have developed an intensity analysis technique for fluorescence microscopy that allows us to measure, in real time, the diameter and the membrane potential or intracellular calcium ([Ca(2+)]i) of in vivo arteriolar endothelium or smooth muscle. Cheek pouch arterioles of anesthetized hamsters were luminally or ablum...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2315
更新日期：2001-07-01 00:00:00
abstract：OBJECTIVE:High aerobic fitness may prevent age-related decrements in cutaneous vasodilation while type 2 diabetes may exacerbate this decline. The mechanisms underlying these responses remain unclear, but may be due to an excess of reactive oxygen species. We hypothesized that superoxide scavenging or NADPH oxidase inh...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103886
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:Studies on the acute effects of coffee on the microcirculation have shown contradicting results. This study aimed to investigate if intake of caffeine-containing coffee changes blood flow and microvascular reactivity in the skin. METHODS:We measured acute changes in cutaneous vascular conductance (CVC) in th...
journal_title：Microvascular research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.mvr.2017.06.006
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.03.007
更新日期：2013-09-01 00:00:00
abstract：:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the pres...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1064
更新日期：1995-11-01 00:00:00
abstract：:We describe a sensitive, reliable, and convenient procedure to assay cell adhesion based on flow cytometry which allows quantitative studies of all major peripheral blood leukocytes adhering to microvascular endothelial cells. Using this assay, we show that adhesion of mononuclear cells is significantly increased in t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0047
更新日期：1996-09-01 00:00:00
