Abstract：
OBJECTIVE:Microvascular perfusion is indispensable for the growth and remodulation of membrane bone. Trauma, inflammation and surgical interventions may alter periosteal perfusion. However, there is not much known about periosteal perfusion in membrane bones. Therefore, the aim of this study was to establish a new chronic model that permits the repeated in vivo analysis of the microcirculation of periosteum. METHODS:A circular skin island with a diameter of 6 mm was excised at the forehead of six Lewis rats. Then a newly developed chamber was implanted, containing a coverglass to protect the periosteum. Intravital microscopy (IVM) enables a quantitative analysis of periosteal microcirculation immediately as well as 3, 5 and 10 days after chamber implantation. At the end of the experiment the calvaria and periosteum were removed for histological examination. Six unoperated Lewis rats served as histological controls. RESULTS:The periosteal microcirculation remained stable over 10 days. The implantation of the chamber did not result in any substantial inflammatory response. The functional microvascular density was 131.2+/-19.3 cm/cm(2). The histological examinations revealed a regular anatomical structure of periosteum and bone including an intact interface. CONCLUSION:The presented model allows for the first time to conduct a repetitive, quantitative in vivo analysis of the periosteal microcirculation in membrane bone. Future studies may thus evaluate novel strategies to influence the periosteal perfusion.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Stuehmer C,Schumann P,Bormann KH,Laschke MW,Menger MD,Gellrich NC,Rücker Mdoi
10.1016/j.mvr.2008.09.008subject
Has Abstractpub_date
2009-03-01 00:00:00pages
104-8issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(08)00145-3journal_volume
77pub_type
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journal_title：Microvascular research
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更新日期：2012-03-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Microvascular research
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1999-11-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2259
更新日期：2000-09-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2432
更新日期：2002-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/mvre.1999.2208
更新日期：2000-01-01 00:00:00
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更新日期：2006-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0026-2862(89)90046-0
更新日期：1989-05-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2045
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1022
更新日期：1993-05-01 00:00:00
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更新日期：2018-07-01 00:00:00
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doi：10.1016/j.mvr.2014.10.006
更新日期：2015-01-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1048
更新日期：1993-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.mvr.2010.03.001
更新日期：2010-07-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2146
更新日期：1999-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.mvr.2009.09.002
更新日期：2009-12-01 00:00:00
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journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90024-x
更新日期：1985-05-01 00:00:00
