Abstract：
:The main objective of this study was to identify molecular mechanisms through which angiopoietin-1 (Ang-1), a ligand for Tie-2 receptors, influences endothelial cell apoptosis. Human umbilical vein endothelial cells were cultured in a medium enriched with 2% fetal bovine serum (FBS) and growth supplements. Apoptosis was induced over 24 h by reducing FBS to 0.1%. Activation of caspase-9, -8, -7, and -3 and the expression of Bcl-2 family proteins, inhibitors of apoptosis (IAPs), cytochrome c, as well as Smac proteins were evaluated with immunoblotting. Ang-1 clearly attenuated serum deprivation-evoked apoptosis, an effect which required Tie-2 receptor activation. Activation of caspase-9, -7, and -3, but not caspase-8, was inhibited by Ang-1. The inhibitory effects of Ang-1 on apoptosis and caspase activation were reversed by a PI-3 kinase inhibitor (wortmannin). Ang-1 exposure upregulated the expression of Survivin but not XIAP (members of IAPs), reduced the cystosolic levels of Smac, but not that of cytochrome c, and had no effect on the expression of Bcl-2 family proteins. This is the first study to report on the mitochondrial mechanisms through which Ang-1 inhibits apoptosis and to investigate the role of the newly discovered Smac. We conclude that Ang-1 inhibits endothelial cell apoptosis through several pathways, which include PI-3 kinase/AKT activation, inhibition of Smac release from the mitochondria, and upregulation of Survivin protein.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Harfouche R,Hasséssian HM,Guo Y,Faivre V,Srikant CB,Yancopoulos GD,Hussain SNdoi
10.1006/mvre.2002.2421keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
135-47issue
1eissn
0026-2862issn
1095-9319pii
S0026286202924210journal_volume
64pub_type
杂志文章abstract：:Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerize...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2449
更新日期：2002-11-01 00:00:00
abstract：:Preeclampsia (PE), a dangerous hypertensive complication of pregnancy, is associated with widespread maternal vascular dysfunction. However, the effect of PE on the cerebral vasculature that can lead to stroke and cognitive decline is not well understood. We hypothesized that function of cortical parenchymal arteriole...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.04.007
更新日期：2018-09-01 00:00:00
abstract：:A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(88)90058-1
更新日期：1988-03-01 00:00:00
abstract：:Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00041-4
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND:Ischemia-reperfusion (IR) injury impairs microcirculatory function by reducing nitric oxide (NO) bioavailability and increasing sympathetic tone. This study non-invasively examined the effects of acute upper limb IR injury on local thermal hyperemia (LTH) in glabrous and non-glabrous finger skin. MATERIALS ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.02.003
更新日期：2018-07-01 00:00:00
abstract：:Particle adhesion in vivo is highly dependent on the microvascular environment comprising of unique anatomical, geometrical, physiological fluid flow conditions and cell-particle and cell-cell interactions. Hence, proper design of vascular-targeted drug carriers that efficiently deliver therapeutics to the targeted ce...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.02.004
更新日期：2015-05-01 00:00:00
abstract：:The steatotic liver is characterized by deranged intrahepatic microvasculature that is believed to predispose it to ischemia-reperfusion injury. The aim of this study was to investigate the distorted hepatic hemodynamics and its impact on the redox status of the steatotic liver. Hepatic hemodynamic parameters, hepatic...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00021-9
更新日期：2003-07-01 00:00:00
abstract：:The steroid hormone estradiol is suggested to play a protective role in intestinal injury during systemic inflammation (sepsis). Our aim was to determine the effects of specific estradiol receptor (ER-α and ER-ß) agonists on the intestinal microcirculation during experimental sepsis. Male and sham ovariectomized femal...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.10.002
更新日期：2013-01-01 00:00:00
abstract：:Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.003
更新日期：2012-09-01 00:00:00
abstract：:Leptin, a circulating hormone secreted mainly from adipose tissues, is involved in the control of body weight. Recently, leptin was found to be an angiogenic factor, and its vitreous levels are associated with angiogenic eye diseases such as proliferative diabetic retinopathy. However, the molecular mechanism for lept...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00005-0
更新日期：2003-05-01 00:00:00
abstract：OBJECTIVE:The present study was examined whether fluvastatin, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, can increase the production of interstitial adenosine via activation of ecto-5'-nucleotidase in the ventricular myocardium, with use of microdialysis techniques in in situ rat hearts. METH...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.04.006
更新日期：2016-09-01 00:00:00
abstract：:The purpose of this study was to elucidate the mechanism by which bradykinin (BK) enhances prostacyclin (PGI(2)) production in human umbilical vein endothelial cells (HUVEC). BK-induced enhancement of PGI(2) synthesis was observed in a dose- and time-dependent manner, and it also increased [Ca(2+)](i) followed by enha...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2163
更新日期：1999-09-01 00:00:00
abstract：:Reperfusion of the ischemic lung is associated with increased pulmonary vascular resistance and reduced alveolar perfusion in conjunction with an inflammatory response. To determine the contribution of platelet-endothelial interactions, we examined effects of pulmonary ischemia-reperfusion (IR) on platelet adhesion an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2003.09.003
更新日期：2004-01-01 00:00:00
abstract：:P120 catenin (p120ctn) belongs to the family of Armadillo repeat-containing proteins, which are believed to have dual functions of cell-cell adhesion and transcriptional regulation. In vascular endothelium, p120ctn is mostly recognized for its cell-cell adhesion function through its ability to regulate VE-cadherin. Th...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.04.001
更新日期：2010-09-01 00:00:00
abstract：:We observed the retinal capillary pericytes of the rat in situ. Whole retinae were mounted, immediately post vivo, in a special tissue chamber for electronic light microscopy at high magnifications. Under electronic light microscopy the pericytes could be clearly distinguished from the endothelial cells. In addition, ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2086
更新日期：1998-07-01 00:00:00
abstract：:The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90060-8
更新日期：1984-05-01 00:00:00
abstract：:Synthetic big endothelin-1 (ET-1), a 39-residue precursor of ET-1, has been reported to elicit potent contractile action on helical strip specimens obtained from the porcine coronary artery, but its molar potency was found to be 140-fold lower than that of ET-1 [Saito, Y., Nakao, K., Mukoyama, M., Imura, H., 1990. Inc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.04.007
更新日期：2006-11-01 00:00:00
abstract：:The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.01.004
更新日期：2010-03-01 00:00:00
abstract：:This study further investigates the mechanisms responsible for the effects of acute and severe hypoxia, and subsequent reoxygenation, on the contractility of isolated rat mesenteric arteries. In noradrenaline (NA)-contracted arteries, hypoxia caused a relaxation to near baseline levels. Reoxygenation resulted in an im...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.08.001
更新日期：2004-11-01 00:00:00
abstract：:Local skin heating to 42°C causes rapid increases in cutaneous perfusion (initial peak), followed by a brief nadir and subsequent sustained elevation (plateau). Several studies have demonstrated that nitric oxide synthase (NOS) largely contributes to the plateau response during local heating. In this study, we tested ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.12.001
更新日期：2018-05-01 00:00:00
abstract：:Capillaries are often assumed to be indistensible. Only recently has the error caused by capillary distensibility in the measurement of hydraulic conductance been considered. An oil-drop method was used to measure simultaneously distensibility and hydraulic conductance (Lp) of mesenteric capillaries of 40 cranially pi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90013-7
更新日期：1989-07-01 00:00:00
abstract：:The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90089-8
更新日期：1992-11-01 00:00:00
abstract：:This paper proposes a mathematical model for vessel recruitment in the microvascular coronary network. The model is based on microvascular network units (MVNUs), where we define a MVNU as a portion of the microvascular network comprising seven generations of identical, parallel-arranged vessels (upstream arteries, lar...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.11.006
更新日期：2016-03-01 00:00:00
abstract：:Previous findings have shown that pulmonary fibroblasts are associated with preexisting holes in the endothelial and epithelial basal laminae through which neutrophils appear to enter and leave the interstitium as they migrate from capillaries to alveoli. To determine their role in neutrophil migration, fibroblast org...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1996.0029
更新日期：1996-05-01 00:00:00
abstract：:We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2356
更新日期：2001-11-01 00:00:00
abstract：:The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90045-1
更新日期：1984-01-01 00:00:00
abstract：:The concept of anti-angiogenesis therapy was introduced by Judah Folkman in 1971 and since then, a plethora of pro- and anti-angiogenic factors have been identified. In the recent years, it has become clear that angiogenesis, the formation of new capillaries from a pre-existing capillary network, is highly regulated b...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2007.05.005
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVES:Calcium channel inhibitors have beneficial impact on microcirculation, but beta-blocker effect is controversial. Clinicians still do not agree on beta-blocker combination with other treatments in the management of impaired microcirculation. The aim of the present study was to describe the effects of beta-blo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.04.004
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:In the present study, we used a two-kidney-two-clip (2k2c) stroke-prone renovascular hypertension rat model (RHRSP) to investigate the protective effects of ligustrazine (TMP) on cerebral arteries and to examine PI3K/Akt pathway behavior under this protection. METHODS:The cerebral artery remodeling was induc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103938
更新日期：2020-03-01 00:00:00
abstract：:The microcirculation in normal and arthritic juxtaarticular bone was studied in 16 young dogs with carragheenan-induced arthritis of one knee. The regional blood flow was determined by the tissue uptake of intracardially injected 15-microns 141Ce-labeled microspheres, and the microvascular plasma volume was determined...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90020-r
更新日期：1990-09-01 00:00:00
