Abstract:
:Electrophysiological characteristics of cultured human umbilical vein endothelial cells (HUVEC) were determined using the patch-clamp technique in the whole cell configuration. In isolated cells, membrane potential, capacitance, and input resistance were (Mean +/- SD) - 16.3 +/- 12.7 mV, 53.9 +/- 26 pF, and 2.3 +/- 1.3 G omega, respectively (N = 26); and in confluent cells - 23.6 +/- 5.5 mV, 127 +/- 59 pF, and 0.254 +/- 0.077 G omega, respectively (N = 6). The almost 10 times higher input resistance, and smaller capacitance of isolated versus confluent cells, indicated that the latter were in electrical communication, presumably through open gap junctions, which was confirmed by intercellular diffusion of Lucifer Yellow. Whole-cell currents of isolated cells were made up of at least three components: First, two outward currents, an early transient one with activation-inactivation kinetics and a small delayed sustained component with 6.75 +/- 4.8 and 0.73 +/- 0.089 nS conductance, respectively. Second, an inward component which was rectified and had 1.58 +/-1.2 nS conductance. In contrast to a reported lack of voltage-gated channels in HUVEC, the above currents were voltage dependent. Inhibition of the whole-cell currents by external Ba2, internal Cs, and other K+ blockers indicates that the three observed currents are carried by K+. This was confirmed by changes of outside K+ concentrations shifting the I-V curve intercept in the direction expected for K(+)-selective channels. Voltage-gated Ca2+ currents were not apparent in the whole-cell current records. HUVEC membrane potential was as low as that of microvascular cells, while inward current rectification at normal external K+ was like that in arterial endothelial cells. This mixed phenotypic expression and multipotential behavior suggests that the electrical features of HUVEC may be primarily determined by embryonic origin and the local effect of the microenvironment rather than strictly by vessel size.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Vargas FF,Caviedes PF,Grant DSdoi
10.1006/mvre.1994.1012subject
Has Abstractpub_date
1994-03-01 00:00:00pages
153-65issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(84)71012-0journal_volume
47pub_type
杂志文章abstract::The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.05.001
更新日期:2004-09-01 00:00:00
abstract::H2O2 mediates autocrine and paracrine signaling in the vasculature and can propagate endothelial dysfunction. However, it is not clear how endothelial cells withstand H2O2 exposure and promote H2O2-induced vascular remodeling. To understand the innate ability of endothelial cells for sustaining excess H2O2 exposure, w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.09.003
更新日期:2016-01-01 00:00:00
abstract::This paper proposes a mathematical model for vessel recruitment in the microvascular coronary network. The model is based on microvascular network units (MVNUs), where we define a MVNU as a portion of the microvascular network comprising seven generations of identical, parallel-arranged vessels (upstream arteries, lar...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.11.006
更新日期:2016-03-01 00:00:00
abstract::Circulation of blood depends, in part, on the ability of red blood cells (RBCs) to aggregate, disaggregate, and deform. The primary intrinsic disaggregating force of RBCs is derived from their electronegativity, which is largely determined by sialylated glycoproteins on the plasma membrane. Given supraphysiological sh...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.008
更新日期:2018-11-01 00:00:00
abstract::A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.02.009
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVE:Vascular endothelial cells (ECs) are constantly exposed to blood flow associated forces such as cyclic strain due to blood pressure, which affects ECs survival and angiogenesis by producing ROS via NAD(P)H oxidase. NAD(P)H oxidase subunit p22phox is reported to be related to the development of atherosclerosis...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2008.08.001
更新日期:2009-03-01 00:00:00
abstract::We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title:Microvascular research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::Interactions between migratory endothelial cells (ECs) and surrounding extracellular matrix (ECM) are of central importance to vascular growth. Here, we present a new model of EC migration and morphogenesis within three-dimensional ECM termed "radial invasion of matrix by aggregated cells" (RIMAC). In the RIMAC model,...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2122
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.03.007
更新日期:2013-09-01 00:00:00
abstract::Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00041-4
更新日期:2003-09-01 00:00:00
abstract::Capillaries are often assumed to be indistensible. Only recently has the error caused by capillary distensibility in the measurement of hydraulic conductance been considered. An oil-drop method was used to measure simultaneously distensibility and hydraulic conductance (Lp) of mesenteric capillaries of 40 cranially pi...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(89)90013-7
更新日期:1989-07-01 00:00:00
abstract::Pericytes are perivascular support cells, the origin of which in tumor tissue is not clear. Recently, we identified a Tie1(+) precursor cell that differentiates into vascular smooth muscle, in a Notch-dependent manner. To understand the involvement of Notch in the ontogeny of tumor pericytes we used a novel flow immun...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.05.004
更新日期:2015-09-01 00:00:00
abstract::Thrombolysis treatment of acute ischemic stroke is limited by the pro-edematous and hemorrhagic effects exerted by reperfusion, which disrupts the blood-brain barrier (BBB) capillary endothelium in the infarct core. Most studies of the ischemic BBB overlook the complexity of the penumbral area, where the affected brai...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.12.002
更新日期:2014-01-01 00:00:00
abstract::Living mesenteric capillaries with either an intact or disrupted glycocalyx were challenged with ramp change in shear stress (Deltatau). Animals (Rana pipiens) were divided randomly into two experimental groups, and two true capillaries (TC) per animal were investigated. The modified Landis technique was combined with...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.12.001
更新日期:2010-03-01 00:00:00
abstract::Alzheimer's disease (AD) is a chronic neuro-degenerative disease that adversely affect many people on a global scale. Despite different diagnostic and therapeutic treatment, there is no cure for AD. The brain is one of the most complex organ and researchers are still trying to understand so as to find a cure. OBJECTI...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.103928
更新日期:2020-03-01 00:00:00
abstract::The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1993.1022
更新日期:1993-05-01 00:00:00
abstract::Non-freezing cold injury (NFCI) is a prevalent, but largely undiagnosed and poorly understood syndrome afflicting many who, as part of their work or leisure, expose their extremities to cold temperatures. The long term sequelae of NFCI are hyperhidrosis, cold-sensitivity and pain; these can last a lifetime. We tested ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.11.002
更新日期:2014-01-01 00:00:00
abstract::In preeclampsia, invasion of intrauterine decidual blood vessels by placental cytotrophoblasts is significantly reduced. This study examined the secretion of matrix metalloproteinases (MMP) by cultured human decidual endothelial cells from normal (NDEC) and preeclamptic (PEDEC) pregnancies. MMPs secreted into the cult...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1998.2142
更新日期:1999-05-01 00:00:00
abstract::Studies of blood flow in mesentery, cremaster muscle, and small bore glass tubes were performed to obtain a relationship between mean velocity (Vmean) and red cell velocity using the two-slit method under epifluorescence (Vepi) and transillumination (Vtrans) microscopy. The velocities Vepi and Vtrans obtained in vivo ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(89)90020-4
更新日期:1989-07-01 00:00:00
abstract::It has generally been assumed that movement of fluid between the pulmonary microvasculature and surrounding tissues is governed by a "Starling" balance of hydrostatic and protein osmotic forces similar to that which prevails in the extremities. However, both recent and older observations suggest that the lungs are mor...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2009.03.004
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVE:In the present study, we used a two-kidney-two-clip (2k2c) stroke-prone renovascular hypertension rat model (RHRSP) to investigate the protective effects of ligustrazine (TMP) on cerebral arteries and to examine PI3K/Akt pathway behavior under this protection. METHODS:The cerebral artery remodeling was induc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2019.103938
更新日期:2020-03-01 00:00:00
abstract::Concentration-response curves are often used as indicators of vascular reactivity and receptor sensitivity. Some investigators use ethylenediaminetetraacetate (EDTA) or ascorbic acid (ASC) to prevent catecholamine oxidation but many others do not. This difference may affect estimates of reactivity. We tested the effec...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(83)90057-2
更新日期:1983-07-01 00:00:00
abstract::Homeostasis around vascular endothelium is a function of the equilibrium between the bioavailability of nitric oxide (NO) and oxidizing reactive oxygen species (ROS). Within the vascular endothelium, NO enhances vasodilatation, reduces platelet aggression and adhesion (anti-thrombotic), prevents smooth muscle prolifer...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2018.03.012
更新日期:2018-09-01 00:00:00
abstract::We evaluated the effect of an oral glucose tolerance test (OGTT) on the level of biomarkers of vascular remodelling. We enrolled 256 Caucasian overweight healthy subjects (H) and 274 overweight type 2 diabetic patients (D). All patients underwent basal measurements of blood glucose (BG), nitrites/nitrates, adiponectin...
journal_title:Microvascular research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.mvr.2010.01.002
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:Endothelial cells are suggested regulators of tumor response to radiation. Anti-vascular targeting agents can enhance tumor response by targeting endothelial cells. Here, we have conducted experiments in vitro to discern the effects of radiation combined with the anti-angiogenic Sunitinib on endothelial (HUV...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2013.10.008
更新日期:2014-03-01 00:00:00
abstract::Age-related macular degeneration (AMD) is a common cause of blindness worldwide. While recent studies have revealed that the loss of choroidal endothelial cells (ChECs) is critical to the disease pathogenesis of dry AMD, in vitro studies are needed to fully elucidate the disease mechanism. However, these studies remai...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.12.002
更新日期:2019-05-01 00:00:00
abstract::The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(85)90040-8
更新日期:1985-07-01 00:00:00
abstract::The contribution of the pleural mesothelium to pleural liquid and protein transport is still vigorously debated. Recent in vitro studies of stripped pleural membrane and free-standing pericardium have demonstrated active ion solute coupled transport of liquid and transcytosis of protein. However, the relative contribu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2005.10.003
更新日期:2005-11-01 00:00:00
abstract:OBJECTIVE:Qualitative capillaroscopy patterns in juvenile- and adult-onset systemic sclerosis (SSc) were studied in adulthood using data from the EULAR Scleroderma Trials and Research (EUSTAR) database. METHODS:Data collected between June 2004 and April 2013 were examined with focus on capillaroscopy. In this retrospe...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.07.007
更新日期:2015-11-01 00:00:00
abstract::We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2017.04.003
更新日期:2017-09-01 00:00:00