Abstract：
:Mesangium enlargement is a central feature of diabetic nephropathy and almost certainly plays a pathogenic role in this condition. Previous studies have shown that mesangium degradation is reduced in a high glucose mileau. Plasmin has been shown to play an important role in extracellular matrix degradation, both directly and through its ability to activate the matrix metalloproteinases. We therefore investigated how high glucose concentration may affect the various components of the plasminogen cascade on mesangial cells and whether it impairs the ability of the mesangial cell to generate plasmin activity. Result showed decreased binding of plasminogen and the urokinase type plasminogen activator to the mesangial cell surface while the tissue type plasminogen activator and the plasminogen activator-1 associated with mesangial cells were increased. The net effect of these changes was a reduced capacity of mesangial cell layers to generate plasmin activity in a high glucose environment. We postulate that this may be of importance in the reduced mesangium degradation which occurs in diabetes.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Fisher EJ,McLennan SV,Yue DK,Turtle JRdoi
10.1006/mvre.1996.2006subject
Has Abstractpub_date
1997-03-01 00:00:00pages
173-8issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(96)92006-3journal_volume
53pub_type
杂志文章abstract：:To investigate the relevance of *NO and oxyradicals in the blood-brain barrier (BBB), differentiated and well-proliferating brain capillary endothelial cells (BCEC) are required. Therefore, rat BCEC (rBCEC) were transfected with immortalizing genes. The resulting lines exhibited endothelial characteristics (factor VII...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2001.2318
更新日期：2001-09-01 00:00:00
abstract：:Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00041-4
更新日期：2003-09-01 00:00:00
abstract：PURPOSE:The conjunctival microcirculation is a readily-accessible vascular bed for quantitative haemodynamic assessment and has been studied previously using a digital charge-coupled device (CCD). Smartphone video imaging of the conjunctiva, and haemodynamic parameter quantification, represents a novel approach. We rep...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103907
更新日期：2019-11-01 00:00:00
abstract：:We have developed a simplified technique for culturing human umbilical vein endothelial cells under shear flow conditions, using prefabricated glass microcapillary tubes ("microslides") with a well-defined rectangular cross section and good optical quality. These microslides have been incorporated into a controlled fl...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1004
更新日期：1993-01-01 00:00:00
abstract：:Cardiovascular diseases, among all diseases, are taking the most victims worldwide. Coronary artery occlusion, takes responsibility of about 30% of the yearly global deaths in the world (Heart Disease and Stroke Statistics 2017 At-a-Glance, 2017), raising the need for viable substitutes for cardiovascular tissues. Dep...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2020.104027
更新日期：2020-09-01 00:00:00
abstract：:Leukocyte margination in the vessels of the microcirculation has been attributed to a flow-dependent interaction with red cells. To determine the extent of this effect, experiments with human blood were done in 100- to 180-micron tubes to detect changes in cell distribution as a function of hematocrit and flow rate. U...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90054-2
更新日期：1984-03-01 00:00:00
abstract：:We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.04.003
更新日期：2017-09-01 00:00:00
abstract：:Carrier-immobilized carbohydrates are valuable tools for assessing the glycoligand-binding capacity of cell surfaces. A panel of 10 types of fluorescent neoglycoproteins has been synthesized to determine the extent of their specific binding to endothelial cells in vitro that have been obtained from porcine brain, ovar...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1053
更新日期：1995-09-01 00:00:00
abstract：:During diabetes in rats, the choroid of the eye shows increased permeability to albumin, basement membrane thickening, and decreased anionic charge sites on the abluminal surfaces of the choriocapillary microvessels. In other microvascular beds, permeability differences are correlated with differences in luminal membr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90058-j
更新日期：1991-11-01 00:00:00
abstract：:The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractil...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1997.2043
更新日期：1997-11-01 00:00:00
abstract：:Focal adhesion kinase (FAK) is a key component of cell-substratum adhesions, known as focal adhesion complexes. Growing evidence indicates that FAK is important in maintenance of normal cell survival and that disruption of FAK signaling results in loss of substrate adhesion and anoikis (apoptosis) of anchorage-depende...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2011.05.003
更新日期：2012-01-01 00:00:00
abstract：:We examined the impact of lymph flow obstruction in large post-nodal lymphatic vessels in sheep. A silk ligature was placed 2 cm downstream from the prescapular or popliteal lymph node and tightened to interrupt flow. At 6, 12 and 16 weeks after lymph flow blockage, a network of small interconnecting lymphatics (appro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.11.003
更新日期：2007-05-01 00:00:00
abstract：:Blood flow studies in the brain, heart, and other organs suggest that there could be interaction between nitric oxide (NO) and adenosine. This possibility was investigated in the optic nerve head (ONH) during photic stimulation of the dark-adapted cat eye. Functional activation of ONH blood flow was measured by laser ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2432
更新日期：2002-09-01 00:00:00
abstract：:Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2010.09.005
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:Qualitative capillaroscopy patterns in juvenile- and adult-onset systemic sclerosis (SSc) were studied in adulthood using data from the EULAR Scleroderma Trials and Research (EUSTAR) database. METHODS:Data collected between June 2004 and April 2013 were examined with focus on capillaroscopy. In this retrospe...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.07.007
更新日期：2015-11-01 00:00:00
abstract：:The microfibrils of anchoring filaments, a typical ultrastructural feature of initial lymphatic vessels, consist mainly of fibrillin and are similar to the microfibrils of elastic fibers. As we previously demonstrated, they radiate from focal adhesions of lymphatic endothelium to the perivascular elastic network. Alth...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2002.2397
更新日期：2002-07-01 00:00:00
abstract：:Pericytes are known to communicate with endothelial cells by direct contact and by releasing cytokines such as TGF-beta. There is also strong evidence that pericytes act as regulators of endothelial cell proliferation and differentiation. We have investigated the effect of pericyte-conditioned medium (PCM) on prolifer...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2208
更新日期：2000-01-01 00:00:00
abstract：:Oxidative stress, which is defined as an imbalance between proxidant and antioxidant systems, is the essential mechanism involving in the ischemic process. During the early stage of brain ischemia, reactive oxygen species (ROS) are increased. Increased ROS are thought of a consequence of brain ischemia and exacerbatin...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.12.005
更新日期：2019-05-01 00:00:00
abstract：:The contractile responses of cultured rat and calf endothelial cells (EC), vascular smooth muscle cells (VSMC), and fibroblasts (FB) to vasoactive mediators (thrombin, serotonin, bradykinin, and histamine), forskolin, and cytochalasin B were compared. Cells were grown on a pliable silicone membrane, and contraction wa...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90015-h
更新日期：1992-03-01 00:00:00
abstract：:Microalbuminuria is a primary manifestation of diabetic nephropathy. Endothelial cell-selective adhesion molecule (ESAM) is a new member of the immunoglobulin superfamily which is selectively expressed by vascular endothelial cells. Although ESAM mediates homophilic interaction between endothelial cells, the role of E...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.01.002
更新日期：2009-05-01 00:00:00
abstract：:The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.01.004
更新日期：2010-03-01 00:00:00
abstract：:Synthetic big endothelin-1 (ET-1), a 39-residue precursor of ET-1, has been reported to elicit potent contractile action on helical strip specimens obtained from the porcine coronary artery, but its molar potency was found to be 140-fold lower than that of ET-1 [Saito, Y., Nakao, K., Mukoyama, M., Imura, H., 1990. Inc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.04.007
更新日期：2006-11-01 00:00:00
abstract：:Leptin, a circulating hormone secreted mainly from adipose tissues, is involved in the control of body weight. Recently, leptin was found to be an angiogenic factor, and its vitreous levels are associated with angiogenic eye diseases such as proliferative diabetic retinopathy. However, the molecular mechanism for lept...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/s0026-2862(03)00005-0
更新日期：2003-05-01 00:00:00
abstract：OBJECTIVE:Leukocytes play a key role in the early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process involves the initiation of the leukocyte adhesion cascade mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.03.007
更新日期：2013-09-01 00:00:00
abstract：:A microfluidic device was developed for quantifying the migratory and deformability capabilities of a single tumor cell using direct imaging. It was fabricated using photolithography and is made of polydimethysiloxane. Chemotaxis approach was used for directing cell movement, using 10 microm microgaps to restrict the ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.06.003
更新日期：2006-11-01 00:00:00
abstract：:The fluidity (the inverse of viscosity) of red blood cell (RBC) suspensions in vivo was estimated by means of microcirculatory measurements such as RBC flow velocity, micropressure in arterioles and venules, and vessel geometry, and by means of simultaneous macrocirculatory measurements such as volume flow and perfusi...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(88)90051-9
更新日期：1988-01-01 00:00:00
abstract：:This article describes the interaction between solid particles and blood propagating through a capillary. A slip condition is considered on the walls of the capillary. The rheological features of the blood are discussed by considering as a two-phase Newtonian fluid model, i.e., the suspension of cells in plasma. A per...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.04.004
更新日期：2018-09-01 00:00:00
abstract：:Strychnine is known to possess anti-inflammatory and antitumour activity, but its roles in tumour angiogenesis, the key step involved in tumour growth and metastasis, and the involved molecular mechanism are still unknown. We aimed to investigate the effects of strychnine on key components of inflammatory angiogenesis...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2013.01.003
更新日期：2013-05-01 00:00:00
abstract：:Endotoxemia, a major feature of sepsis, is a common cause of acute lung injury and initiates rapid accumulation of leukocytes in the lung vasculature. Endothelial mechanisms that underlie this accumulation remain unclear, as current experimental models of endotoxemia are less suitable for targeted activation of the en...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.01.006
更新日期：2012-05-01 00:00:00
abstract：:Hyperoxia and hyperbaric oxygen therapy can restore oxygen tensions in tissues distressed by ischemic injury and poor vascularization and is believed to also yield angiogenesis and regulate tissue perfusion. The aim of this study was to develop a model in which hyperoxia-driven microvascular changes could be quantifie...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.02.001
更新日期：2016-05-01 00:00:00
