Abstract:
:Carrier-immobilized carbohydrates are valuable tools for assessing the glycoligand-binding capacity of cell surfaces. A panel of 10 types of fluorescent neoglycoproteins has been synthesized to determine the extent of their specific binding to endothelial cells in vitro that have been obtained from porcine brain, ovary, and testis. Different sugar moieties revealed a nonuniform capacity to bind to the endothelial cells as determined by flow cytometric analysis, the histogenetic origin of the preparations being an important factor. Binding of mannose, xylose, and glucuronic acid was especially pronounced, but the extent of cell-associated fluorescence was significantly different dependent on the source of the endothelial cells. These results clearly reveal that endothelial cells in vitro display the capacity to specifically recognize defined carbohydrate moieties. Moreover, endothelial cells of different tissue origin can exhibit variability in this property, potentially endowing this cell type with site-dependent molecular properties.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Plendl J,Sinowatz F,Auerbach R,Gabius HJdoi
10.1006/mvre.1995.1053subject
Has Abstractpub_date
1995-09-01 00:00:00pages
199-214issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(85)71053-9journal_volume
50pub_type
杂志文章abstract::The aim of this study is to develop a novel non-contrast 4-dimensional MR arterial spin labeling (4D-ASL) technique (3D acquisition and time) and to investigate myocardial perfusion on healthy volunteers without administration of contrast materials. A non-contrast 4D-ASL technique was developed using the time-spatial ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.01.007
更新日期:2015-03-01 00:00:00
abstract::We tested the hypothesis that cationic peptides (polyarginine; poly-argn) and eosinophil-derived major basic protein (MaBP) increase permeability by stimulating the release of histamine and/or nitric oxide. We used intravital microscopy, clearance, and integrated optical intensity (IOI), using FITC-dextran 150 (FITC-d...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1038
更新日期:1995-07-01 00:00:00
abstract::A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.02.009
更新日期:2009-06-01 00:00:00
abstract::Pericytes are known to communicate with endothelial cells by direct contact and by releasing cytokines such as TGF-beta. There is also strong evidence that pericytes act as regulators of endothelial cell proliferation and differentiation. We have investigated the effect of pericyte-conditioned medium (PCM) on prolifer...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1999.2208
更新日期:2000-01-01 00:00:00
abstract::Routine pulmonary ultrasound for diagnosis of disease or injury relies on interpretation of image features, such as comet-tail artifacts, which can also be indicative of the poorly understood phenomenon of ultrasound-induced pulmonary capillary hemorrhage (PCH). Evans blue extraction and bronchoalveolar lavage (BAL) w...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.02.006
更新日期:2014-05-01 00:00:00
abstract::We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2001.2356
更新日期:2001-11-01 00:00:00
abstract::Endothelial cells and endothelial progenitor cells (EPCs) play a key role in the pathogenesis of vascular disease. Both cell types are affected by the oxidative stress but their susceptibility may be different. This study aimed to investigate the antioxidative enzymes activated in EPCs after high constant glucose expo...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.05.004
更新日期:2010-12-01 00:00:00
abstract::7-Ketocholesterol (7kCh) is a major oxysterol found associated with vascular diseases. Human microvascular endothelial cells (HMVECs) were cultured with different concentrations of 7kCh with and without inhibitors. Cell viabilities and caspase activities were assessed. 7kCh caused loss of cell viability in a dose-depe...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2007.10.003
更新日期:2008-04-01 00:00:00
abstract::We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title:Microvascular research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis in the mammalian eye, suggesting that loss of PEDF is implicated in the pathogenesis of proliferative diabetic retinopathy. However, a role for PEDF in early diabetic retinopathy remains to be elucidated. Since oxidative stress is thought t...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.11.001
更新日期:2005-01-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the correlation between the expression of miR-210 in peripheral blood and the number of peripheral endothelial progenitor cells (EPCs) in patients with type 2 diabetes mellitus (T2DM). We also determined the effect of miR-210 on EPC proliferation, adhesion, migration, tube forma...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2020.104032
更新日期:2020-09-01 00:00:00
abstract::The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(92)90089-8
更新日期:1992-11-01 00:00:00
abstract::Reduced lung capillary expression of angiotensin I-converting enzyme (ACE), a key enzyme in cardiovascular pathophysiology, and of caveolin-1, an important regulator of endothelial cell signalling, has been demonstrated in various models of pulmonary arterial hypertension (PAH). We addressed the relationship between P...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.04.008
更新日期:2010-09-01 00:00:00
abstract::Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1993.1016
更新日期:1993-03-01 00:00:00
abstract::The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.2000.2236
更新日期:2000-07-01 00:00:00
abstract::Circulation of blood depends, in part, on the ability of red blood cells (RBCs) to aggregate, disaggregate, and deform. The primary intrinsic disaggregating force of RBCs is derived from their electronegativity, which is largely determined by sialylated glycoproteins on the plasma membrane. Given supraphysiological sh...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.05.008
更新日期:2018-11-01 00:00:00
abstract::The effect of hemodilution on oxygen delivery to the tissue was investigated analytically by taking into consideration the oxygen loss that occurs along the arterial microvasculature due to diffusion into the tissue and shunting to parallel running venules. The theoretical findings were related to experimental data on...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(87)90062-8
更新日期:1987-11-01 00:00:00
abstract::Sanguinate, a polyethylene glycol-conjugated carboxyhemoglobin, was investigated for cerebral vasoactivity in healthy male Sprague-Dawley rats (Study 1) and for its ability to increase brain tissue oxygen pressure (PbtO2) after controlled cortical impact (CCI) - traumatic brain injury (TBI) (Study 2). In both studies ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2016.06.001
更新日期:2016-09-01 00:00:00
abstract::Oxidative stress, which is defined as an imbalance between proxidant and antioxidant systems, is the essential mechanism involving in the ischemic process. During the early stage of brain ischemia, reactive oxygen species (ROS) are increased. Increased ROS are thought of a consequence of brain ischemia and exacerbatin...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2018.12.005
更新日期:2019-05-01 00:00:00
abstract::Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2009.07.006
更新日期:2009-12-01 00:00:00
abstract::The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.01.004
更新日期:2010-03-01 00:00:00
abstract::It has generally been assumed that movement of fluid between the pulmonary microvasculature and surrounding tissues is governed by a "Starling" balance of hydrostatic and protein osmotic forces similar to that which prevails in the extremities. However, both recent and older observations suggest that the lungs are mor...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2009.03.004
更新日期:2009-06-01 00:00:00
abstract::The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2004.05.001
更新日期:2004-09-01 00:00:00
abstract::Leukocyte margination in the vessels of the microcirculation has been attributed to a flow-dependent interaction with red cells. To determine the extent of this effect, experiments with human blood were done in 100- to 180-micron tubes to detect changes in cell distribution as a function of hematocrit and flow rate. U...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(84)90054-2
更新日期:1984-03-01 00:00:00
abstract::Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.03.001
更新日期:2010-07-01 00:00:00
abstract::Changes in blood vessel diameter can be measured manually, but this is time-consuming and often impractical. For automatic measurement commercial solutions are available, but the proprietary algorithms and their potential shortcomings are not known to the user. We present an approach with a CCD camera for image acquis...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2010.04.004
更新日期:2010-09-01 00:00:00
abstract::An increasing body of evidence suggests that the deleterious effects of Acute Kidney Injury (AKI) on remote organ function could, at least in part, be due to loss of the normal balance of immune, inflammatory, and soluble mediator metabolism that attends injury of the tubular epithelium. Such dysregulation, acting at ...
journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2008.09.001
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:The present study was examined whether fluvastatin, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, can increase the production of interstitial adenosine via activation of ecto-5'-nucleotidase in the ventricular myocardium, with use of microdialysis techniques in in situ rat hearts. METH...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2016.04.006
更新日期:2016-09-01 00:00:00
abstract::Rho-associated kinases are involved in regulation of actin-myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influenc...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/s0026-2862(03)00041-4
更新日期:2003-09-01 00:00:00
abstract::Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly when fluorescent visu...
journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(88)90022-2
更新日期:1988-11-01 00:00:00