当前位置： SCI文献检索 > MICROVASCULAR RESEARCH期刊下所有文献 > *NO and oxyradical metabolism in new cell lines of rat brain capillary endothelial cells forming the blood-brain barrier.

*NO and oxyradical metabolism in new cell lines of rat brain capillary endothelial cells forming the blood-brain barrier.

Abstract：

:To investigate the relevance of *NO and oxyradicals in the blood-brain barrier (BBB), differentiated and well-proliferating brain capillary endothelial cells (BCEC) are required. Therefore, rat BCEC (rBCEC) were transfected with immortalizing genes. The resulting lines exhibited endothelial characteristics (factor VIII, angiotensin-converting enzyme, high prostacyclin/thromboxane release rates) and BBB markers (gamma-glutamyl transpeptidase, alkaline phosphatase). The control line rBCEC2 (mock transfected) revealed fibroblastoid morphology, less factor VIII, reduced gamma-glutamyl transpeptidase, weak radical defence, low prostanoid metabolism, and limited proliferation. Lines transfected with immortalizing genes (especially rBCEC4, polyoma virus large T antigen) conserved primary properties: epitheloid morphology, subcultivation with high proliferation rate under pure culture conditions, and powerful defence against reactive oxygen species (Mn-, Cu/Zn-superoxide dismutase, catalase, glutathione peroxidase, glutathione) effectively controlling radical metabolism. Only 100 microM H2O2 overcame this defence and stimulated the formation of eicosanoids similarly as in primary cells. Some BBB markers were expressed to a lower degree; however, cocultivation with astrocytes intensified these markers (e.g., alkaline phosphatase) and paraendothelial tightness, indicating induction of BBB properties. Inducible NO synthase was induced by a cytokine plus lipopolysaccharide mixture in all lines and primary cells, resulting in *NO release. Comparing the cell lines obtained, rBCEC4 are stable immortalized and reveal the best conservation of properties from primary cells, including enzymes producing or decomposing reactive species. These cells can be subcultivated in large amounts and, hence, they are suitable to study the role of radical metabolism in the BBB and in the cerebral microvasculature.

journal_name

Microvasc Res

journal_title

Microvascular research

authors

Blasig IE,Giese H,Schroeter ML,Sporbert A,Utepbergenov DI,Buchwalow IB,Neubert K,Schönfelder G,Freyer D,Schimke I,Siems WE,Paul M,Haseloff RF,Blasig R

doi

10.1006/mvre.2001.2318

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

114-27

issue

2

eissn

0026-2862

issn

1095-9319

pii

S0026-2862(01)92318-0

journal_volume

62

pub_type

杂志文章

相关文献

MICROVASCULAR RESEARCH文献大全
  • Vascular architecture in free flaps: Analysis of vessel morphology and morphometry in murine free flaps.

    abstract：:The aim of this study was to analyze the development of vascular architecture as well as vascular morphometry and morphology of anastomosed microvascular free flaps. Free pectoral skin flaps were raised in 25 rats and anastomosed to the femoral vessels in the groin region. CD31 immunohistology was performed after 3, 7...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2018.03.006

    authors： Pabst AM,Kämmerer PW,Krüger M,Jäger L,Ackermann M

    更新日期：2018-07-01 00:00:00

  • Influence of some hydrodynamic factors of thrombus formation in microvessels.

    abstract：:The effect of mean blood velocity on thrombus formation parameters was studied using mesenteric vessels of white rats. An advanced phenomenological theory, explaining the relationship between mean blood velocity and thrombus growth rate, is presented. The qualitative analysis of theoretical and experimental data have ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1995.1002

    authors： Petrishchev NN,Mikhailova IA

    更新日期：1995-01-01 00:00:00

  • A novel population of local pericyte precursor cells in tumor stroma that require Notch signaling for differentiation.

    abstract：:Pericytes are perivascular support cells, the origin of which in tumor tissue is not clear. Recently, we identified a Tie1(+) precursor cell that differentiates into vascular smooth muscle, in a Notch-dependent manner. To understand the involvement of Notch in the ontogeny of tumor pericytes we used a novel flow immun...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2015.05.004

    authors： Patenaude A,Woerher S,Umlandt P,Wong F,Ibrahim R,Kyle A,Unger S,Fuller M,Parker J,Minchinton A,Eaves CJ,Karsan A

    更新日期：2015-09-01 00:00:00

  • IL-20 activates human lymphatic endothelial cells causing cell signalling and tube formation.

    abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2009.02.007

    authors： Hammer T,Tritsaris K,Hübschmann MV,Gibson J,Nisato RE,Pepper MS,Dissing S

    更新日期：2009-06-01 00:00:00

  • Augmentation of oxygen transport by various hemoglobins as determined by pulsed field gradient NMR.

    abstract：:Diffusion of oxyhemoglobin has been shown to augment the oxygen transport inside the red blood cells. Measurement of hemoglobin diffusion coefficients by pulsed-field gradient (PFG) nuclear magnetic resonance (NMR) technique can be used for estimating this augmentation effect. Self-diffusion coefficients of polymerize...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.2002.2449

    authors： Budhiraja V,Hellums JD,Post JF

    更新日期：2002-11-01 00:00:00

  • Partial chemical characterization of the anionic sites in the basal lamina of fenestrated capillaries.

    abstract：:The distribution of anionic sites in the basal laminae of the blood capillaries of the murine pancreas was studied in specimens fixed in ruthenium red (RR)-glutaraldehyde mixtures. The sites appeared as discrete, small (6 to 18 nm) particles distributed throughout the three laminae but concentrated primarily in the la...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(84)90006-2

    authors： Simionescu M,Simionescu N,Palade GE

    更新日期：1984-11-01 00:00:00

  • Wavelet coherence analysis of spontaneous oscillations in cerebral tissue oxyhemoglobin concentrations and arterial blood pressure in elderly subjects.

    abstract：:This study aims to assess the relationship between spontaneous oscillations in changes in cerebral tissue oxyhemoglobin concentrations (Delta [HbO2]) and arterial blood pressure (ABP) signals in healthy elderly subjects during the resting state using wavelet coherence analysis. Continuous recordings of near-infrared s...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2014.02.008

    authors： Cui R,Zhang M,Li Z,Xin Q,Lu L,Zhou W,Han Q,Gao Y

    更新日期：2014-05-01 00:00:00

  • Effect of dispersion of vessel diameters and lengths in stochastic networks. II. Modeling of microvascular hematocrit distribution.

    abstract：:A microvascular network model described in the preceding paper (B. Dawant, M. Levin, and A. S. Popel, 1986, Microvasc. Res. 31, 203-222) featuring random distribution of vessel diameters and lengths, is extended to calculate the distributions of red blood cell flux and discharge hematocrit throughout the network. A re...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(86)90036-1

    authors： Levin M,Dawant B,Popel AS

    更新日期：1986-03-01 00:00:00

  • Feasibility of assessment of conjunctival microvascular hemodynamics in unilateral ischemic stroke.

    abstract：:Since the internal carotid artery supplies blood to both the eye and the brain, ocular microvascular hemodynamics can be altered due to ischemic stroke. The purpose of the current study was to establish the feasibility of conjunctival microcirculation imaging for detection of inter-ocular differences in microvascular ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2015.04.007

    authors： Kord Valeshabad A,Wanek J,Mukarram F,Zelkha R,Testai FD,Shahidi M

    更新日期：2015-07-01 00:00:00

  • Endothelial cells from diverse tissues exhibit differences in growth and morphology.

    abstract：:An increased recognition of the role of endothelial cells in disease and the development of methods for endothelial cell culture has led to an upsurge in in vitro studies of endothelial cell function. However, the cells most often used for these studies do not reflect the in vivo heterogeneity of endothelial cells. To...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1997.2045

    authors： Craig LE,Spelman JP,Strandberg JD,Zink MC

    更新日期：1998-01-01 00:00:00

  • Anti-vascular endothelial growth factor treatment induces blood flow recovery through vascular remodeling in high-fat diet induced diabetic mice.

    abstract：:Diabetes mellitus (DM) leads to the development of microvascular diseases and is associated with impaired angiogenesis. The presence of vascular endothelial growth factor (VEGF) can block PDGF-BB dependent regulation of neovascularization and vessel normalization. We tested the hypothesis that the inhibition of VEGF i...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2016.01.005

    authors： Xiao L,Yan K,Yang Y,Chen N,Li Y,Deng X,Wang L,Liu Y,Mu L,Li R,Luo M,Ren M,Wu J

    更新日期：2016-05-01 00:00:00

  • Ramp acceleration and hydraulic conductivity (L(p)) of living capillaries.

    abstract：:Living mesenteric capillaries with either an intact or disrupted glycocalyx were challenged with ramp change in shear stress (Deltatau). Animals (Rana pipiens) were divided randomly into two experimental groups, and two true capillaries (TC) per animal were investigated. The modified Landis technique was combined with...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2009.12.001

    authors： Williams DA

    更新日期：2010-03-01 00:00:00

  • Effects of ascorbic acid and EDTA on vascular concentration-response to catecholamines.

    abstract：:Concentration-response curves are often used as indicators of vascular reactivity and receptor sensitivity. Some investigators use ethylenediaminetetraacetate (EDTA) or ascorbic acid (ASC) to prevent catecholamine oxidation but many others do not. This difference may affect estimates of reactivity. We tested the effec...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(83)90057-2

    authors： Maxwell LC,Herlihy JT,Riedel GL

    更新日期：1983-07-01 00:00:00

  • Source of angiopoietin-2 in the sera of women during pregnancy.

    abstract：:Placental development requires coordinated angiogenesis regulated by multiple factors including angiopoietins. Previously we demonstrated that the concentration of angiopoietin-2 (Ang-2) in the sera of women rises markedly in pregnancy in early gestation. This increase is reduced in pregnancies subsequently complicate...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2012.08.003

    authors： Woolnough C,Wang Y,Kan CY,Morris JM,Tasevski V,Ashton AW

    更新日期：2012-11-01 00:00:00

  • Comparison between pore model predictions and sheep lung fluid and protein transport.

    abstract：:The multiple pore model of T. R. Harris and R. J. Roselli (1981, J. Appl. Physiol: Respir. Environ. Exercise Physiol. 50, 1-14), was used to simulate lung lymph flow and protein transport at various levels of microvascular pressure. Response of the three-pore structure determined in that study was found to be in excel...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(85)90022-6

    authors： Roselli RJ,Parker RE,Harris TR

    更新日期：1985-05-01 00:00:00

  • A novel method for evaluation of capillarity in human skeletal muscles from confocal 3D images.

    abstract：:A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green)...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2010.11.012

    authors： Janáček J,Cvetko E,Kubínová L,Travnik L,Eržen I

    更新日期：2011-03-01 00:00:00

  • Spectral analysis of the blood flow in the foot microvascular bed during thermal testing in patients with diabetes mellitus.

    abstract：:Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In thi...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2018.05.005

    authors： Mizeva I,Zharkikh E,Dremin V,Zherebtsov E,Makovik I,Potapova E,Dunaev A

    更新日期：2018-11-01 00:00:00

  • A quantitative observation and imaging of single tumor cell migration and deformation using a multi-gap microfluidic device representing the blood vessel.

    abstract：:A microfluidic device was developed for quantifying the migratory and deformability capabilities of a single tumor cell using direct imaging. It was fabricated using photolithography and is made of polydimethysiloxane. Chemotaxis approach was used for directing cell movement, using 10 microm microgaps to restrict the ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2006.06.003

    authors： Chaw KC,Manimaran M,Tay FE,Swaminathan S

    更新日期：2006-11-01 00:00:00

  • Use of tadalafil in a patient with a secondary Raynaud's phenomenon not responding to sildenafil.

    abstract：:The Raynaud's phenomenon often accompanies systemic rheumatic diseases and is also known as a vascular side effect of chemotherapy. Therapy of the Raynaud's phenomenon with nitrates or calcium-channel-blockers is rarely beneficial. In contrast, the PDE-V-inhibitor sildenafil seems to be effective in these patients. Fo...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2005.03.001

    authors： Baumhaekel M,Scheffler P,Boehm M

    更新日期：2005-05-01 00:00:00

  • Chronic elevations in salt intake and reduced renal mass hypertension compromise mechanisms of arteriolar dilation.

    abstract：:Alterations in the reactivity of arterioles to vasodilator agonists were assessed in the skeletal muscle microcirculation of age-matched, normotensive male Sprague-Dawley rats fed either a high salt (4% NaCl, HS) or a low salt (0.4% NaCl, LS) diet and in reduced renal mass hypertensive rats on a high salt diet (HSRRM)...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1998.2105

    authors： Frisbee JC,Lombard JH

    更新日期：1998-11-01 00:00:00

  • A simplified method for culture of endothelial cells and analysis of adhesion of blood cells under conditions of flow.

    abstract：:We have developed a simplified technique for culturing human umbilical vein endothelial cells under shear flow conditions, using prefabricated glass microcapillary tubes ("microslides") with a well-defined rectangular cross section and good optical quality. These microslides have been incorporated into a controlled fl...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1993.1004

    authors： Cooke BM,Usami S,Perry I,Nash GB

    更新日期：1993-01-01 00:00:00

  • Expression of adhesion molecules by cultured human glomerular endothelial cells in response to cytokines: comparison to human umbilical vein and dermal microvascular endothelial cells.

    abstract：:We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but ...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.2001.2356

    authors： Murakami S,Morioka T,Nakagawa Y,Suzuki Y,Arakawa M,Oite T

    更新日期：2001-11-01 00:00:00

  • Chronic oxidative-nitrosative stress impairs coronary vasodilation in metabolic syndrome model rats.

    abstract：:Metabolic syndrome (MetS) is a combination of clinical disorders that together increase the risk for cardiovascular disease and diabetes. SHRSP.Z-Lepr(fa)/IzmDmcr (SHRSP.ZF) rats with MetS show impaired nitric oxide-mediated relaxation in coronary and mesenteric arteries, and angiotensin II receptor type 1 blockers pr...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2013.04.001

    authors： Kagota S,Maruyama K,Tada Y,Fukushima K,Umetani K,Wakuda H,Shinozuka K

    更新日期：2013-07-01 00:00:00

  • Altered regulator mechanisms of presynaptic adrenergic nerve: a new physiopathological hypothesis in Raynaud's disease.

    abstract：:The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(84)90045-1

    authors： Giovanni B,Giuseppina CM,Susanna F,Roberto M

    更新日期：1984-01-01 00:00:00

  • Type IV collagen-derived angiogenesis inhibitors.

    abstract：:The concept of anti-angiogenesis therapy was introduced by Judah Folkman in 1971 and since then, a plethora of pro- and anti-angiogenic factors have been identified. In the recent years, it has become clear that angiogenesis, the formation of new capillaries from a pre-existing capillary network, is highly regulated b...

    journal_title：Microvascular research

    pub_type： 杂志文章,评审

    doi：10.1016/j.mvr.2007.05.005

    authors： Mundel TM,Kalluri R

    更新日期：2007-09-01 00:00:00

  • Acute VEGF effect on solute permeability of mammalian microvessels in vivo.

    abstract：:To investigate the effect of vascular endothelial growth factor (VEGF) on solute permeability of mammalian microvessels, we measured the apparent permeability (P) of various-sized solutes on the postcapillary venules of rat mesentery in vivo. Exposure to 1 nM VEGF transiently increased P from a mean of 1.4 (+/-0.11 SE...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2004.03.004

    authors： Fu BM,Shen S

    更新日期：2004-07-01 00:00:00

  • Spontaneous cyclic contractions of the capillary wall in vivo, impeding red cell flow: a quantitative analysis. Evidence for endothelial contractility.

    abstract：:We have videorecorded spontaneous cyclic contractions of capillary walls which often stopped the flow of blood cells, in spleens of rat and mouse. An inverted microscope and oblique lighting were key elements in obtaining images in which the boundaries of cells composing vessel walls were clearly distinguishable. Usin...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/0026-2862(88)90035-0

    authors： Ragan DM,Schmidt EE,MacDonald IC,Groom AC

    更新日期：1988-07-01 00:00:00

  • Pericyte adhesion and growth onto polyhydroxymethylsiloxane surfaces nanostructured by plasma treatment and ion irradiation.

    abstract：:The study deals with the adhesion and proliferation of bovine retina pericytes onto surfaces of poly(hydroxymethylsiloxane) (PHMS) modified either by cold plasma or by low-energy ion beams. The surface treatment was able to convert the original polymer matrix into SiO2-like phases for O2-plasma or ion-mixed SiCxOy(Hz)...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2004.08.003

    authors： Assero G,Satriano C,Lupo G,Anfuso CD,Marletta G,Alberghina M

    更新日期：2004-11-01 00:00:00

  • The lymphatic vessels and the so-called "lymphatic stomata" of the diaphragm: a morphologic ultrastructural and three-dimensional study.

    abstract：:We studied the absorbing peripheral lymphatic vessel with the light microscope, the transmission electron microscope, the scanning electron microscope, and three-dimensional models of the diaphragm of several rodents and insectivores under normal and experimental conditions (lymphatic stasis and dehydration). To clari...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1006/mvre.1998.2101

    authors： Azzali G

    更新日期：1999-01-01 00:00:00

  • Differences in skin blood flow oscillations between the plantar and dorsal foot in people with diabetes mellitus and peripheral neuropathy.

    abstract：BACKGROUND:Understanding the differences in skin blood flow (SBF) on the plantar and dorsal foot in people with diabetes mellitus (DM) may help to assess the influence of diabetes and neuropathy on microvascular dysfunction and risks of diabetic foot ulcers in this population. However, there is no study comparing SBF o...

    journal_title：Microvascular research

    pub_type： 杂志文章

    doi：10.1016/j.mvr.2018.11.002

    authors： Jan YK,Liao F,Cheing GLY,Pu F,Ren W,Choi HMC

    更新日期：2019-03-01 00:00:00