Abstract：
:The study deals with the adhesion and proliferation of bovine retina pericytes onto surfaces of poly(hydroxymethylsiloxane) (PHMS) modified either by cold plasma or by low-energy ion beams. The surface treatment was able to convert the original polymer matrix into SiO2-like phases for O2-plasma or ion-mixed SiCxOy(Hz) phases for ion irradiation, respectively, with different modification levels of the surface free energy (SFE) and related surface wettability. Pericytes exhibited a negligible adhesion and proliferation onto untreated PHMS, an enhanced adhesion but not proliferation on plasma-treated PHMS, and great adhesion and proliferation to full confluence on ion-irradiated PHMS, as measured by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), quartz crystal microbalance, and optical microscopy. On the other hand, the adhesion and proliferation of GP8.39 endothelial cells (EC), which are strongly associated with pericytes in microvasculature, were very scarce onto both untreated and surface-modified PHMS. The surface-selective pericytal response was related to changes of physicochemical properties of PHMS film, from hydrophobic/neutral towards hydrophilic/negatively charged polymer layers, as well as to short- and long-time events of cell-surface interaction. We propose that surface properties can mediate and modulate cell-polymer matrix adhesion through the establishment of stereospecific chemical interactions and/or electrostatic repulsion, which can also explain the different behavior of pericytes compared to EC.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Assero G,Satriano C,Lupo G,Anfuso CD,Marletta G,Alberghina Mdoi
10.1016/j.mvr.2004.08.003keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
209-20issue
3eissn
0026-2862issn
1095-9319pii
S0026-2862(04)00115-3journal_volume
68pub_type
杂志文章abstract：:Normal brain parenchyma has a highly complex microvascular structure that undergoes re-modelling in the presence of a tumor. A detailed understanding of the clinical and pathological aspects of neo-angiogenesis in malignant human brain tumors is essential in order to ensure the successful clinical application of anti-...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2010.09.005
更新日期：2010-12-01 00:00:00
abstract：:Controversy exists concerning whether activation of N-methyl-D-aspartate (NMDA) receptors exerts direct dilator effects on cerebral arteries. The purpose of this study was to examine the responses of isolated piglet arteries to NMDA to determine whether isolated arteries, apart from surrounding neuronal tissue, are ca...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.007
更新日期：2005-07-01 00:00:00
abstract：:We tested the hypothesis that the endothelial nitric oxide (NO)-soluble guanylyl cyclase system is involved in atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) mediated regulation of coronary and peripheral vascular resistance. Rat hearts were perfused via the aorta at constant flow and the effect...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2289
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:Microvascular perfusion is indispensable for the growth and remodulation of membrane bone. Trauma, inflammation and surgical interventions may alter periosteal perfusion. However, there is not much known about periosteal perfusion in membrane bones. Therefore, the aim of this study was to establish a new chro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.09.008
更新日期：2009-03-01 00:00:00
abstract：:Patients with diabetic polyneuropathy are known to have an impaired neurovascular reflex arc compared to healthy controls. This is seen in a delayed decrease in microcirculation of the ipsilateral hand after cooling of the contralateral hand. The aim of this pilot study was to investigate whether intravenous alpha-lip...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2151
更新日期：1999-07-01 00:00:00
abstract：:HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the pres...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1064
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVE:To assess the morphology of the lymphatic system pre- and postoperatively in patients undergoing microsurgical reconstructions of the lymphatic vessels. MATERIALS AND METHODS:8 lower extremities in 4 consecutive patients with secondary unilateral lymphedema of the lower extremities were examined by MR lympha...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.03.003
更新日期：2008-05-01 00:00:00
abstract：:A microfluidic device was developed for quantifying the migratory and deformability capabilities of a single tumor cell using direct imaging. It was fabricated using photolithography and is made of polydimethysiloxane. Chemotaxis approach was used for directing cell movement, using 10 microm microgaps to restrict the ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.06.003
更新日期：2006-11-01 00:00:00
abstract：:Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of ne...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.12.001
更新日期：2015-03-01 00:00:00
abstract：:Fluid homeostasis is required for life. Processes involved in fluid balance are strongly related to exchanges at the microvascular level. Computational models have been presented in the literature to analyze the microvascular-interstitial interactions. As far as we know, none of those models consider a physiological d...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.11.003
更新日期：2019-03-01 00:00:00
abstract：:The purpose of this study was to determine if there are intrinsic differences in resting tone, vascular reactivity, myogenic responses, and neurogenic vasoconstriction between the large and small feeder arteries and first order arterioles (1A) of the rat cremaster muscle. The pudic-epigastric artery (PEA), external sp...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2259
更新日期：2000-09-01 00:00:00
abstract：:We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title：Microvascular research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：PURPOSE:To investigate conjunctival microvascular responses in patients with mild diabetic retinopathy (MDR) and methylenetetrahydrofolate reductase (MTHFR) polymorphisms (D + PM) after administration of Ocufolin™, a medical food containing 900 μg l-methylfolate (levomefolate calcium or [6S]-5-methyltetrahydrofolic aci...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104066
更新日期：2020-11-01 00:00:00
abstract：:Pericytes are perivascular support cells, the origin of which in tumor tissue is not clear. Recently, we identified a Tie1(+) precursor cell that differentiates into vascular smooth muscle, in a Notch-dependent manner. To understand the involvement of Notch in the ontogeny of tumor pericytes we used a novel flow immun...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.05.004
更新日期：2015-09-01 00:00:00
abstract：:Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.011
更新日期：2012-11-01 00:00:00
abstract：:Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1029
更新日期：1994-05-01 00:00:00
abstract：:Age-related macular degeneration (AMD) is a common cause of blindness worldwide. While recent studies have revealed that the loss of choroidal endothelial cells (ChECs) is critical to the disease pathogenesis of dry AMD, in vitro studies are needed to fully elucidate the disease mechanism. However, these studies remai...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.12.002
更新日期：2019-05-01 00:00:00
abstract：:Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis in the mammalian eye, suggesting that loss of PEDF is implicated in the pathogenesis of proliferative diabetic retinopathy. However, a role for PEDF in early diabetic retinopathy remains to be elucidated. Since oxidative stress is thought t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.11.001
更新日期：2005-01-01 00:00:00
abstract：:IL-20 is an arteriogenic cytokine that remodels collateral networks in vivo, and plays a role in cellular organization. Here, we investigate its role in lymphangiogenesis using a lymphatic endothelial cell line, hTERT-HDLEC, which expresses the lymphatic markers LYVE-1 and podoplanin. Upon stimulation of hTERT-HDLEC w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：:Ischemia/Reperfusion injury and hemolysis are characterized by erythrocyte lysis and release of free heme into the microcirculation. Following substantial erythrocyte lysis, heme overwhelms circulatory heme-binding protein networks rapidly forming hemin, the oxidized form of iron protoporphyrin IX. Hemin's role in mod...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2007.01.002
更新日期：2007-05-01 00:00:00
abstract：:Thermal injuries of more than 20% body surface area (BSA) result in systemic capillary leakage with subsequent edema. This can similarly be induced by burn plasma transfer (BPT) from burned individuals to healthy rats. We evaluated if cerium nitrate (CN) bathing can prevent edema after BPT. Therefore, donor rats (DR) ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.07.006
更新日期：2009-12-01 00:00:00
abstract：:Particle adhesion in vivo is highly dependent on the microvascular environment comprising of unique anatomical, geometrical, physiological fluid flow conditions and cell-particle and cell-cell interactions. Hence, proper design of vascular-targeted drug carriers that efficiently deliver therapeutics to the targeted ce...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.02.004
更新日期：2015-05-01 00:00:00
abstract：:Quantum dots (QDs) are fluorescent semiconductor nanocrystals that possess a number of superior fluorescent properties compared to more established organic dyes and fluorescent proteins. As a result, QDs are being studied for use in a wide range of biological applications. We have examined QDs for one such application...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.09.003
更新日期：2007-03-01 00:00:00
abstract：:We have used ruthenium red, a cationic dye, to detect at the electron microscopic level the presence of anionic sites in various murine basement membranes, with particular emphasis on those of the microvasculature. We have observed anionic sites in all continuous and fenestrated capillaries examined. Terminal lymphati...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90018-3
更新日期：1983-05-01 00:00:00
abstract：:H2O2 mediates autocrine and paracrine signaling in the vasculature and can propagate endothelial dysfunction. However, it is not clear how endothelial cells withstand H2O2 exposure and promote H2O2-induced vascular remodeling. To understand the innate ability of endothelial cells for sustaining excess H2O2 exposure, w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.09.003
更新日期：2016-01-01 00:00:00
abstract：AIMS:The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. METHODS:Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR); diabetes+sham (D +...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104009
更新日期：2020-07-01 00:00:00
abstract：:Bile duct obstruction is associated with hepatic accumulation of leukocytes and liver injury. Emerging data suggest that platelets may play an important role in tissue damage and inflammation. Herein, we characterized the platelet response in cholestatic liver injury and evaluated the role of Rho-kinase signaling. For...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.04.003
更新日期：2009-06-01 00:00:00
abstract：:Placental development requires coordinated angiogenesis regulated by multiple factors including angiopoietins. Previously we demonstrated that the concentration of angiopoietin-2 (Ang-2) in the sera of women rises markedly in pregnancy in early gestation. This increase is reduced in pregnancies subsequently complicate...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.08.003
更新日期：2012-11-01 00:00:00
abstract：:In this paper we quantitatively investigate the hypothesis proposed by Michel (Exp. Physiol. 82, 1-30, 1997) and Weinbaum (Ann. Biomed. Eng. 26, 1-17, 1998) that the Starling forces are determined by the local difference in the hydrostatic and colloid osmotic pressure across the endothelial surface glycocalyx, which w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2177
更新日期：1999-11-01 00:00:00
abstract：:The study of angiogenesis as a therapeutic target requires reliable in vivo assays that can provide physiologically relevant data. A murine in vivo Matrigel-based angiogenesis assay is presented here which includes the quantitative assessment of vascular-specific indicators of neovascularization. Matrigel containing 1...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.01.002
更新日期：2006-03-01 00:00:00
