Abstract:
:We investigated the expression of cell adhesion molecules on the surface of glomerular endothelial cells (GEC), dermal microvascular endothelial cells (MvE), and umbilical vein endothelial cells (HUVEC) that had or had not been stimulated by cytokines. PECAM-1 was constitutively expressed at a high level on HUVEC but its expression level decreased following stimulation by tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma). PECAM-1 was also constitutively expressed on microvascular endothelial cells MvE and GEC, but at lower levels than on HUVEC, and expression by these cells also decreased in response to TNF-alpha and IFN-gamma. There was no dose-dependent effect on MvE but there was a dose-dependent effect on the level of expression of cell adhesion molecules on GEC. TNF-alpha induced the expression of VCAM-1 on HUVEC and GEC, but not MvE, while IFN-gamma induced VCAM-1 expression only on HUVEC. TNF-alpha induced the expression of E-selectin on all three kinds of endothelial cells, but IFN-gamma had no effect on E-selectin expression. GEC therefore showed expression patterns of PECAM-1, VCAM-1, and E-selectin different from those seen in HUVEC and MvE upon treatment with TNF-alpha or IFN-gamma. The use of cultured human GEC allows us to study not only the inflammatory processes, but also the pathophysiological role of GEC in hemodynamic disturbances and their interaction with intrinsic mesangial cells at the molecular and subcellular levels.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Murakami S,Morioka T,Nakagawa Y,Suzuki Y,Arakawa M,Oite Tdoi
10.1006/mvre.2001.2356keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
383-91issue
3eissn
0026-2862issn
1095-9319pii
S0026-2862(01)92356-8journal_volume
62pub_type
杂志文章abstract::This study aims to assess the relationship between spontaneous oscillations in changes in cerebral tissue oxyhemoglobin concentrations (Delta [HbO2]) and arterial blood pressure (ABP) signals in healthy elderly subjects during the resting state using wavelet coherence analysis. Continuous recordings of near-infrared s...
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1994.1063
更新日期:1994-11-01 00:00:00
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journal_title:Microvascular research
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doi:10.1016/j.mvr.2008.02.004
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/0026-2862(91)90058-j
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journal_title:Microvascular research
pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Microvascular research
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doi:10.1006/mvre.2000.2259
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journal_title:Microvascular research
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doi:10.1006/mvre.2001.2363
更新日期:2002-01-01 00:00:00
abstract::We have applied theories of mass transfer, in laminar and turbulent flow, to red cells in the stopped-flow apparatus in order to estimate the effect of extracellular diffusion boundary layers on the initial rate of O2 uptake. We compared the theoretical predictions with the results of our previous stopped-flow experim...
journal_title:Microvascular research
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doi:10.1016/0026-2862(83)90058-4
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pub_type: 杂志文章,评审
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更新日期:2018-09-01 00:00:00
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doi:10.1016/j.mvr.2015.07.007
更新日期:2015-11-01 00:00:00
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doi:10.1016/j.mvr.2011.07.008
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2006.04.007
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journal_title:Microvascular research
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doi:10.1016/j.mvr.2013.04.001
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.08.003
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1995.1038
更新日期:1995-07-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2015.02.004
更新日期:2015-05-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2012.10.005
更新日期:2013-01-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1006/mvre.1993.1060
更新日期:1993-11-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章,评审
doi:10.1016/j.mvr.2007.03.004
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2005.03.001
更新日期:2005-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.mvr.2015.09.003
更新日期:2016-01-01 00:00:00