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Intracellular localization of lipoplexed siRNA in vascular endothelial cells of different mouse tissues.

Abstract：

:Liposomally formulated siRNA can be used for RNAi applications in vivo. Intravenous bolus administration of lipoplexed siRNA has been shown to reduce gene expression in the vascular endothelium. Here, we applied immunofluorescence staining for different endothelial markers (PECAM-1, CD34, laminin) on paraffin sections to compare the respective expression pattern with the intracellular localization of intravenously administered, fluorescently labeled siRNA (siRNA-Cy3-lipoplex). By confocal microscopy, lipoplexed siRNA-Cy3 was detected inside vascular endothelial cells in vivo, which where identified with co-staining of endothelial markers. Consequently, the finding of intracellular siRNA uptake by vascular endothelial cells correlated with RNAi based specific protein reduction in situ as revealed by PECAM-1 specific immunofluorescence staining in lung tissue sections. Therefore, by using a cell biological approach these in situ data emphasize the functional uptake of liposomal siRNA molecules in vascular endothelial cells of different mouse tissues as indicated in our previous molecular study.

journal_name

Microvasc Res

journal_title

Microvascular research

authors

Aleku M,Fisch G,Möpert K,Keil O,Arnold W,Kaufmann J,Santel A

doi

10.1016/j.mvr.2008.02.004

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

31-41

issue

1

eissn

0026-2862

issn

1095-9319

pii

S0026-2862(08)00029-0

journal_volume

76

pub_type

杂志文章

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