Abstract:
PURPOSE:To examine the possible role of Klotho (Kl) in human microvasculature. METHODS:The expression level of Kl in primary human dermal microvascular endothelial cells (HDMECs) and primary human dermal fibroblasts (HFb) was detected by real-time polymerase chain reaction amplification (qRT-PCR), Western blot analyses and immunohistochemistry. Migration of HDMECs and HFb was examined in monolayer wound healing "scratch assay" and Transwell assay. Proliferation of these cells was examined using Cell Proliferation BrdU incorporation assay. RESULTS:Our results have shown that downregulation of Kl abrogated HDMECs migration after 48h. On the other hand, migration of HFb significantly increased after blocking Kl. Lack of Kl decreased expression of genes involved in the activation of endothelial cells and enhanced expression of genes involved in extracellular matrix remodeling and organization of connective tissue. CONCLUSIONS:This study for the first time provides the evidence that Kl is expressed in HDMECs and HFb. Additionally, we have demonstrated that Kl is implicated in the process of angiogenesis of human dermal microvasculature.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Markiewicz M,Panneerselvam K,Marks Ndoi
10.1016/j.mvr.2016.05.005subject
Has Abstractpub_date
2016-09-01 00:00:00pages
76-82eissn
0026-2862issn
1095-9319pii
S0026-2862(16)30052-8journal_volume
107pub_type
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journal_title:Microvascular research
pub_type: 杂志文章
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journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Microvascular research
pub_type: 杂志文章
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journal_title:Microvascular research
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2018.10.007
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journal_title:Microvascular research
pub_type: 杂志文章
doi:10.1016/j.mvr.2014.05.005
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journal_title:Microvascular research
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journal_title:Microvascular research
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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