Abstract:
:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT12) superfamily of transporters were compared across physiological stages of buffalo mammary gland. The relative expression of ABCA1, and ABCG1 was significantly (p < 0.05) higher in mammary gland of heifer followed by involution and lactation stages. Similarly, ABCA7 gene expression was highest in heifer mammary gland followed by lactation and involution stages. ABCG2 gene expression was significantly (p < 0.05) high in lactating mammary gland in comparison to involution and heifer stages. On the other hand, ABCG5 gene expression was highest in involuting mammary gland followed by lactation and involution stages. Additionally, the expression of LXRα SREBF1, and PPARA which are known to regulate some of the ABC tranporters were also analyzed. The expression of LXRα gene was high in involuting as compared to lactating mammary gland. In contrast, SREBF1 and PPARA expression was significantly (p < 0.05) high in lactating mammary gland. Among the several SLC transporters studied, SLC2A1, SLC2A4, and SLC2A8 showed significant (p < 0.05) higher expression during lactation stage, whereas SLC2A12 expression was greater during heifer stage suggesting SLC2A1, SLC2A4, and SLC2A8 to be the major transporters associated with glucose uptake in buffalo mammary gland. The expression profile of (lactoferrin) LTF, known to be expressed at high level in mammary gland during involution was also studied. As expected, its expression was significantly (p < 0.05) higher during involution in comparison to lactating mammary gland.in buffaloes as well. The inclusion of LTF as a control gene further provided the confidence in the buffalo mammary gland expression data generated in the present study. This study thus helped to provide information about the distinct expression pattern of various transporters and their regulators in buffalo mammary gland during different physiological states.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh Mdoi
10.1080/10495398.2013.839949subject
Has Abstractpub_date
2014-01-01 00:00:00pages
200-9issue
3eissn
1049-5398issn
1532-2378journal_volume
25pub_type
杂志文章abstract::Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1687091
更新日期:2019-11-18 00:00:00
abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1863225
更新日期:2021-01-10 00:00:00
abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1329150
更新日期:2018-04-03 00:00:00
abstract::This study elucidated the molecular markers that decrease oocyte quality during in vitro culture, restricting optimal developmental potential. Here, we evaluated the transcriptomic differences between cysteamine-treated and non-treated bovine cumulus oocyte complexes (COCs) after 22 h of co-culture in the maturation m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1706545
更新日期:2020-01-03 00:00:00
abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1803892
更新日期:2020-08-13 00:00:00
abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437046
更新日期:2019-01-01 00:00:00
abstract::The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495391003608316
更新日期:2010-04-01 00:00:00
abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525887
更新日期:1998-01-01 00:00:00
abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601091040
更新日期:2007-01-01 00:00:00
abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601115526
更新日期:2007-01-01 00:00:00
abstract::The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1242490
更新日期:2017-04-03 00:00:00
abstract::In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.653462
更新日期:2012-01-01 00:00:00
abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120026481
更新日期:2003-11-01 00:00:00
abstract::The effects of supplementing diet of growing lambs with microbial feed additive mixture (direct-fed microbial; DFM) based on Saccharomyces cerevisiae, lactic acid bacteria and exogenous enzymes on feed intake, nutrient digestibility, nitrogen (N) utilization, ruminal fermentation, blood chemistry and growth performanc...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1616554
更新日期:2020-10-01 00:00:00
abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1735404
更新日期:2020-04-02 00:00:00
abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500461104
更新日期:2006-01-01 00:00:00
abstract::Indigenous cattle of India belong to the species, Bos indicus and they possess various adaptability and production traits. However, little is known about the genetic diversity and origin of these breeds. To investigate the status, we sequenced and analyzed the whole mitochondrial DNA (mtDNA) of seven Indian cattle bre...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1476376
更新日期:2019-07-01 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1796697
更新日期:2020-08-09 00:00:00
abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601088657
更新日期:2007-01-01 00:00:00
abstract::Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprin...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1074915
更新日期:2016-01-01 00:00:00
abstract::A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1182540
更新日期:2016-10-01 00:00:00
abstract::A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1027774
更新日期:2015-01-01 00:00:00
abstract::The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1784185
更新日期:2020-07-07 00:00:00
abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525921
更新日期:1999-01-01 00:00:00
abstract::Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120037897
更新日期:2004-05-01 00:00:00
abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
更新日期:1998-01-01 00:00:00
abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1135807
更新日期:2016-01-01 00:00:00
abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
abstract::Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/LABT-200036706
更新日期:2004-01-01 00:00:00
abstract::Retinoid X receptor α (RXRα) is thought to be a key regulator in lipid metabolism, glucose metabolism, adipogenic differentiation, gene expression, and inflammatory response. However, it is not clear whether RXRα has any role in intramuscular preadipocyte of goat. In the current investigation, we report that adenoviru...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1732397
更新日期:2020-02-27 00:00:00