Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines.

abstract：:The pig is a recognized model for the onset of coronary heart disease and heart attacks. Previous studies have shown that serum cholesterol levels in the pig can be elevated using a high fat, high cholesterol (HFHC) diet. What has been lacking is a genetically defined model corresponding to human ApoE4 susceptibility ...

journal_title：Animal biotechnology

authors： Jensen TW,Mazur MJ,Pettigew JE,Perez-Mendoza VG,Zachary J,Schook LB

更新日期：2010-07-01 00:00:00

abstract：:This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

journal_title：Animal biotechnology

authors： Li Y,Yang H,Xia L,Wang S,Bu S

更新日期：2020-03-17 00:00:00

abstract：:Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

journal_title：Animal biotechnology

authors： Liu W,Lamont SJ

更新日期：2003-05-01 00:00:00

abstract：:Indigenous cattle of India belong to the species, Bos indicus and they possess various adaptability and production traits. However, little is known about the genetic diversity and origin of these breeds. To investigate the status, we sequenced and analyzed the whole mitochondrial DNA (mtDNA) of seven Indian cattle bre...

journal_title：Animal biotechnology

authors： Pramod RK,Velayutham D,P K S,P S B,Zachariah A,Zachariah A,B C,S S S,P G,Dhinoth Kumar B,Iype S,Gupta R,Santhosh S,Thomas G

更新日期：2019-07-01 00:00:00

abstract：:Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

journal_title：Animal biotechnology

authors： Nygard AB,Jørgensen CB,Cirera S,Fredholm M

更新日期：2010-10-01 00:00:00

abstract：:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

journal_title：Animal biotechnology

authors： Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

更新日期：2014-01-01 00:00:00

abstract：:The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...

journal_title：Animal biotechnology

authors： Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

更新日期：2012-04-01 00:00:00

abstract：:Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

journal_title：Animal biotechnology

authors： Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

更新日期：2005-01-01 00:00:00

abstract：:In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Dall'Olio S,Buttazzoni L,Gallo M,Russo V

更新日期：2012-01-01 00:00:00

abstract：:Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

journal_title：Animal biotechnology

authors： Wang Z,Rohrer G,Stone R,Troyer D

更新日期：2000-01-01 00:00:00

abstract：:The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...

journal_title：Animal biotechnology

authors： Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G

更新日期：2020-02-12 00:00:00

abstract：:Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

journal_title：Animal biotechnology

authors： Shi X,Xia C,Pan B,Wang M

更新日期：2006-01-01 00:00:00

abstract：:The present study was aimed at genetic characterization and diversity analysis of Pandharpuri buffalo population using the Food and Agriculture Organization (FAO) recommended bovine microsatellite markers. Genomic DNA was isolated from blood samples of 50 unrelated animals and a total of 23 microsatellite loci were am...

journal_title：Animal biotechnology

authors： Khade KA,Panigrahi M,Ahmad SF,Kumar P,Bhushan B

更新日期：2020-10-01 00:00:00

abstract：:Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

journal_title：Animal biotechnology

authors： Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

更新日期：2019-01-01 00:00:00

abstract：:The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...

journal_title：Animal biotechnology

authors： Lal SV,Mukherjee A,Brahma B,Gohain M,Patra MC,Saini SK,Mishra P,Ahlawat S,Upadhyaya RC,Datta TK,De S

更新日期：2016-01-01 00:00:00

abstract：:For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

journal_title：Animal biotechnology

authors： Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

更新日期：2007-01-01 00:00:00

abstract：:Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

journal_title：Animal biotechnology

authors： Rahimi H,Tukmechi A,Rashidian E

更新日期：2020-06-10 00:00:00

abstract：:Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

journal_title：Animal biotechnology

authors： Salem M,Nath J,Killefer J

更新日期：2004-01-01 00:00:00

abstract：:A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

journal_title：Animal biotechnology

authors： Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

更新日期：2002-05-01 00:00:00

abstract：:The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...

journal_title：Animal biotechnology

authors： Laliotis GP,Marantidis A,Avdi M

更新日期：2017-04-03 00:00:00

abstract：:We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

journal_title：Animal biotechnology

authors： Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

更新日期：2011-01-01 00:00:00

abstract：:The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

journal_title：Animal biotechnology

authors： Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

更新日期：2011-07-01 00:00:00

abstract：:In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 express...

journal_title：Animal biotechnology

authors： Kho Y,Kim S,Yoon BS,Moon JH,Kwak S,Park G,Woo J,Oh S,Hong K,Kim S,Kim H,You S,Choi Y

更新日期：2008-01-01 00:00:00

abstract：:Growth arrest and DNA-damage-inducible gamma (GADD45G) is a reproduction related gene. In this study, the full-length cDNA sequence of porcine GADD45G gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine GADD45G gene encodes a protein of 159 amino acids that shares high homology with the...

journal_title：Animal biotechnology

authors： Liu C,Zhang W,Yang D,Liu Y

更新日期：2015-01-01 00:00:00

abstract：:The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using ...

journal_title：Animal biotechnology

authors： Zi XD,Chen SW,Liang GN,Chen DW,Zhang DW,Yin RH

更新日期：2009-01-01 00:00:00

abstract：:The associations between polymorphisms of five genes, calpain 1 (CAPN1), follicle stimulating hormone beta (FSHB), follicle stimulating hormone receptor (FSHR), peroxisome proliferator-activated receptor gamma (PPARG), and retinol binding protein 7 (RBP7), and live weight, carcass composition, and meat-quality traits ...

journal_title：Animal biotechnology

authors： Sun Y,Liu R,Lu X,Hu Y,Zhao G,Zheng M,Chen J,Wang H,Wen J

更新日期：2013-01-01 00:00:00

abstract：:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

journal_title：Animal biotechnology

authors： Agca C,Bidwell CA,Donkin SS

更新日期：2004-05-01 00:00:00

abstract：:A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

journal_title：Animal biotechnology

authors： Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

更新日期：2015-01-01 00:00:00

abstract：:The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

journal_title：Animal biotechnology

authors： Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

更新日期：2001-11-01 00:00:00

abstract：:Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length...

journal_title：Animal biotechnology

authors： Kumar R,Ramteke PW,Sharma SK,Mitra A

更新日期：2015-01-01 00:00:00