Abstract:
:The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observed for the examined loci, while the B allele noted to be the more frequent in all analyzed genes. In addition, sows with the AA genotype of BF gene found to produce significantly lower numbers of the total born piglets (TNB) and number of piglets born alive (TNA), while the respective BB genotype significantly exceeded in TNB and NBA traits compared to the other two genotypes (P < 0.001). In the case of RBP4 gene, sows with the AB genotype noted to have significant higher values of TNB and NBA in regard to the respective homozygous genotypes (AA or BB), while the BB genotype showed intermediate results (P < 0.001). There were no statistical differences between the BB genotype and the AB genotype of ESR2 locus in regard to the examined traits. However, a noticeable superiority (P < 0.01) of the BB genotype compared to the homozygous AA genotype, adding almost 2 piglets/litter in TNB and NBA trait, was found. ABBREVIATIONS:TNB: Total number of born piglets; NBA: Number of piglets born alive.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Laliotis GP,Marantidis A,Avdi Mdoi
10.1080/10495398.2016.1242490subject
Has Abstractpub_date
2017-04-03 00:00:00pages
138-143issue
2eissn
1049-5398issn
1532-2378journal_volume
28pub_type
杂志文章abstract::Growth arrest and DNA-damage-inducible gamma (GADD45G) is a reproduction related gene. In this study, the full-length cDNA sequence of porcine GADD45G gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine GADD45G gene encodes a protein of 159 amino acids that shares high homology with the...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1005216
更新日期:2015-01-01 00:00:00
abstract::Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1811294
更新日期:2020-08-31 00:00:00
abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601115526
更新日期:2007-01-01 00:00:00
abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1777149
更新日期:2020-06-10 00:00:00
abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
更新日期:2008-01-01 00:00:00
abstract::The pig is a recognized model for the onset of coronary heart disease and heart attacks. Previous studies have shown that serum cholesterol levels in the pig can be elevated using a high fat, high cholesterol (HFHC) diet. What has been lacking is a genetically defined model corresponding to human ApoE4 susceptibility ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2010.490693
更新日期:2010-07-01 00:00:00
abstract::Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120037897
更新日期:2004-05-01 00:00:00
abstract::Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1687091
更新日期:2019-11-18 00:00:00
abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1823400
更新日期:2020-09-28 00:00:00
abstract::Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1157081
更新日期:2016-01-01 00:00:00
abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1735404
更新日期:2020-04-02 00:00:00
abstract::Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390009525945
更新日期:2000-01-01 00:00:00
abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-200053398
更新日期:2005-01-01 00:00:00
abstract::A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1182540
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.630897
更新日期:2012-01-01 00:00:00
abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
更新日期:2012-04-01 00:00:00
abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525887
更新日期:1998-01-01 00:00:00
abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1313269
更新日期:2018-04-03 00:00:00
abstract::Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2013.796960
更新日期:2014-01-01 00:00:00
abstract::Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprin...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1074915
更新日期:2016-01-01 00:00:00
abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500461104
更新日期:2006-01-01 00:00:00
abstract::The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.581558
更新日期:2011-07-01 00:00:00
abstract::This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1723608
更新日期:2020-03-17 00:00:00
abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
更新日期:1998-01-01 00:00:00
abstract::The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495391003608316
更新日期:2010-04-01 00:00:00
abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1271805
更新日期:2017-10-02 00:00:00
abstract::The effects of supplementing diet of growing lambs with microbial feed additive mixture (direct-fed microbial; DFM) based on Saccharomyces cerevisiae, lactic acid bacteria and exogenous enzymes on feed intake, nutrient digestibility, nitrogen (N) utilization, ruminal fermentation, blood chemistry and growth performanc...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1616554
更新日期:2020-10-01 00:00:00
abstract::The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1800485
更新日期:2020-07-31 00:00:00
abstract::Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1433191
更新日期:2019-01-01 00:00:00