Production of single-chain fragment variable (scFv) antibodies specific to plasma membrane epitopes on bull Y-bearing sperm.

abstract：:Retinoid X receptor α (RXRα) is thought to be a key regulator in lipid metabolism, glucose metabolism, adipogenic differentiation, gene expression, and inflammatory response. However, it is not clear whether RXRα has any role in intramuscular preadipocyte of goat. In the current investigation, we report that adenoviru...

journal_title：Animal biotechnology

authors： Xu Q,Wang Y,Zhang Y,Zhu J,Lin Y

更新日期：2020-02-27 00:00:00

abstract：:Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

journal_title：Animal biotechnology

authors： Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

更新日期：2005-01-01 00:00:00

abstract：:P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...

journal_title：Animal biotechnology

authors： He X,Li B,Fu S,Wang B,Qi Y,Da L,Te R,Sun S,Liu Y,Zhang W

更新日期：2019-07-18 00:00:00

abstract：:Medicinal plants exhibit colossal impact on poultry industries by improving its performance and productivity. However, some of these plants show adverse influence too by decreasing egg production percentage, egg mass, and microbiota counts. Green tea, nettle, pennyroyal, yarrow, and alfalfa in the form of seed, powder...

journal_title：Animal biotechnology

authors： Pliego AB,Tavakoli M,Khusro A,Seidavi A,Elghandour MMMY,Salem AZM,Márquez-Molina O,Rene Rivas-Caceres R

更新日期：2020-08-03 00:00:00

abstract：:Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

journal_title：Animal biotechnology

authors： Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

更新日期：2017-01-02 00:00:00

abstract：:The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

journal_title：Animal biotechnology

authors： Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

更新日期：2020-07-31 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

journal_title：Animal biotechnology

authors： Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

更新日期：2020-08-09 00:00:00

abstract：:This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

journal_title：Animal biotechnology

authors： Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

更新日期：2018-01-01 00:00:00

abstract：:The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

journal_title：Animal biotechnology

authors： Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

更新日期：2019-01-01 00:00:00

abstract：:This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

journal_title：Animal biotechnology

authors： Li Y,Yang H,Xia L,Wang S,Bu S

更新日期：2020-03-17 00:00:00

abstract：:A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

journal_title：Animal biotechnology

authors： Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

更新日期：2015-01-01 00:00:00

abstract：:A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...

journal_title：Animal biotechnology

authors： Prinzenberg EM,Krause I,Erhardt G

更新日期：1999-01-01 00:00:00

abstract：:The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

journal_title：Animal biotechnology

authors： Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

更新日期：2013-01-01 00:00:00

abstract：:The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...

journal_title：Animal biotechnology

authors： Austbø L,Røed KH,Dolvik NI,Skretting G

更新日期：2010-04-01 00:00:00

abstract：:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

journal_title：Animal biotechnology

authors： Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

更新日期：2014-01-01 00:00:00

abstract：:Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

journal_title：Animal biotechnology

authors： Bauer MM,Miller MM,Briles WE,Reed KM

更新日期：2013-01-01 00:00:00

abstract：:A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

journal_title：Animal biotechnology

authors： Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

更新日期：2008-01-01 00:00:00

abstract：:The associations between polymorphisms of five genes, calpain 1 (CAPN1), follicle stimulating hormone beta (FSHB), follicle stimulating hormone receptor (FSHR), peroxisome proliferator-activated receptor gamma (PPARG), and retinol binding protein 7 (RBP7), and live weight, carcass composition, and meat-quality traits ...

journal_title：Animal biotechnology

authors： Sun Y,Liu R,Lu X,Hu Y,Zhao G,Zheng M,Chen J,Wang H,Wen J

更新日期：2013-01-01 00:00:00

abstract：:Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

journal_title：Animal biotechnology

authors： Reed KM,Chaves LD

更新日期：2008-01-01 00:00:00

abstract：:The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

journal_title：Animal biotechnology

authors： Tantia MS,Vijh RK,Mishra B,Kumar ST

更新日期：2007-01-01 00:00:00

abstract：:Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

journal_title：Animal biotechnology

authors： Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

更新日期：2020-08-13 00:00:00

abstract：:High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...

journal_title：Animal biotechnology

authors： Wang Y,Gao Q,Cheng X,Chang G,Roy AC,Shen X

更新日期：2021-01-24 00:00:00

abstract：:The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...

journal_title：Animal biotechnology

authors： Awotunde EO,Bemji MN,Olowofeso O,James IJ,Ajayi OO,Adebambo AO

更新日期：2015-01-01 00:00:00

abstract：:Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...

journal_title：Animal biotechnology

authors： Migdal L,Koziol K,Palka S,Migdal W,Zabek T,Otwinowska-Mindur A,Migdal A,Kmiecik M,Maj D,Bieniek J

更新日期：2018-04-03 00:00:00

abstract：:CARP, ANKRD2, and DARP belong to the ankyrin repeat protein (MARP) family and play a critical role in the integration of cytoskeletal architecture, stress response, and transcriptional regulation. In this study, we cloned the cDNA and promoter sequences of porcine ankyrin repeat protein (MARP) gene family. RT-PCR anal...

journal_title：Animal biotechnology

authors： Wang L,Lei M,Xiong Y

更新日期：2011-04-01 00:00:00

abstract：:Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

journal_title：Animal biotechnology

authors： Wang Z,Rohrer G,Stone R,Troyer D

更新日期：2000-01-01 00:00:00

abstract：:The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

journal_title：Animal biotechnology

authors： Ji S,Willis GM,Scott RR,Spurlock ME

更新日期：1998-01-01 00:00:00

abstract：:Prolactin is a versatile hormone with multiple activities, including a negative control on egg production. This study was conducted to genotype all the coding portions of the prl gene using PCR-SSCP-sequencing, and to investigate the effects of amino acid substitutions of the prl gene on the structure and function of ...

journal_title：Animal biotechnology

authors： Al-Shuhaib MBS,Al-Kafajy FR,Al-Jashami GS

更新日期：2019-10-29 00:00:00

abstract：BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

journal_title：Animal biotechnology

authors： Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

更新日期：2012-01-01 00:00:00

abstract：:In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Dall'Olio S,Buttazzoni L,Gallo M,Russo V

更新日期：2012-01-01 00:00:00