Isolation of thirty-one new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library.

abstract：:The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...

journal_title：Animal biotechnology

authors： Gupta T,Rawat M,Kumar B,Varshney R,Chakravarti S,Qureshi S

更新日期：2020-04-02 00:00:00

abstract：:Prolactin is a versatile hormone with multiple activities, including a negative control on egg production. This study was conducted to genotype all the coding portions of the prl gene using PCR-SSCP-sequencing, and to investigate the effects of amino acid substitutions of the prl gene on the structure and function of ...

journal_title：Animal biotechnology

authors： Al-Shuhaib MBS,Al-Kafajy FR,Al-Jashami GS

更新日期：2019-10-29 00:00:00

abstract：:Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

journal_title：Animal biotechnology

authors： Liu W,Lamont SJ

更新日期：2003-05-01 00:00:00

abstract：:The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

journal_title：Animal biotechnology

authors： Ji S,Willis GM,Scott RR,Spurlock ME

更新日期：1998-01-01 00:00:00

abstract：:Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

journal_title：Animal biotechnology

authors： Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

更新日期：2021-01-18 00:00:00

abstract：:The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

journal_title：Animal biotechnology

authors： Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

更新日期：2011-07-01 00:00:00

abstract：:Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was...

journal_title：Animal biotechnology

authors： Chen X,Luo Y,Jia G,Liu G,Zhao H,Huang Z

更新日期：2017-10-02 00:00:00

abstract：:This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

journal_title：Animal biotechnology

authors： Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

更新日期：2018-01-01 00:00:00

abstract：:Growth arrest and DNA-damage-inducible gamma (GADD45G) is a reproduction related gene. In this study, the full-length cDNA sequence of porcine GADD45G gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine GADD45G gene encodes a protein of 159 amino acids that shares high homology with the...

journal_title：Animal biotechnology

authors： Liu C,Zhang W,Yang D,Liu Y

更新日期：2015-01-01 00:00:00

abstract：:Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

journal_title：Animal biotechnology

authors： Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

更新日期：2019-01-01 00:00:00

abstract：:Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a ...

journal_title：Animal biotechnology

authors： Zhang S,Chen X,Huang Z,Chen D,Yu B,He J,Zheng P,Yu J,Luo J,Luo Y,Chen H

更新日期：2018-07-03 00:00:00

abstract：:Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

journal_title：Animal biotechnology

authors： Salem M,Nath J,Killefer J

更新日期：2004-01-01 00:00:00

abstract：:Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...

journal_title：Animal biotechnology

authors： Yasue H,Mikawa S,Uenishi H,Wada Y

更新日期：1999-01-01 00:00:00

abstract：:Retinoid X receptor α (RXRα) is thought to be a key regulator in lipid metabolism, glucose metabolism, adipogenic differentiation, gene expression, and inflammatory response. However, it is not clear whether RXRα has any role in intramuscular preadipocyte of goat. In the current investigation, we report that adenoviru...

journal_title：Animal biotechnology

authors： Xu Q,Wang Y,Zhang Y,Zhu J,Lin Y

更新日期：2020-02-27 00:00:00

abstract：:The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...

journal_title：Animal biotechnology

authors： Civánová K,Knoll A

更新日期：2007-01-01 00:00:00

abstract：:Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

journal_title：Animal biotechnology

authors： Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

更新日期：2005-01-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

journal_title：Animal biotechnology

authors： Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

更新日期：2019-01-01 00:00:00

abstract：:The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...

journal_title：Animal biotechnology

authors： Austbø L,Røed KH,Dolvik NI,Skretting G

更新日期：2010-04-01 00:00:00

abstract：:A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...

journal_title：Animal biotechnology

authors： Wu H,Bi X,Cao F,Zhu C,Liu H,Song J,Ma L,Ma L,Zhang Y,Zhao D,Liu H,Xu X,Zhang S

更新日期：2016-10-01 00:00:00

abstract：:The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

journal_title：Animal biotechnology

authors： Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

更新日期：2020-07-31 00:00:00

abstract：:Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

journal_title：Animal biotechnology

authors： Shi X,Xia C,Pan B,Wang M

更新日期：2006-01-01 00:00:00

abstract：:To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

journal_title：Animal biotechnology

authors： Burk NE,Messer LA,Ernst CW,Rothschild MF

更新日期：1998-01-01 00:00:00

abstract：:Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length...

journal_title：Animal biotechnology

authors： Kumar R,Ramteke PW,Sharma SK,Mitra A

更新日期：2015-01-01 00:00:00

abstract：:The associations between polymorphisms of five genes, calpain 1 (CAPN1), follicle stimulating hormone beta (FSHB), follicle stimulating hormone receptor (FSHR), peroxisome proliferator-activated receptor gamma (PPARG), and retinol binding protein 7 (RBP7), and live weight, carcass composition, and meat-quality traits ...

journal_title：Animal biotechnology

authors： Sun Y,Liu R,Lu X,Hu Y,Zhao G,Zheng M,Chen J,Wang H,Wen J

更新日期：2013-01-01 00:00:00

abstract：:Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

journal_title：Animal biotechnology

authors： Reed KM,Chaves LD

更新日期：2008-01-01 00:00:00

abstract：:The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

journal_title：Animal biotechnology

authors： Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

更新日期：2019-01-01 00:00:00

abstract：:Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

journal_title：Animal biotechnology

authors： Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

更新日期：2017-01-02 00:00:00

abstract：:Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...

journal_title：Animal biotechnology

authors： Campbell BT,Kojima CJ,Cooper TA,Bastin BC,Wojakiewicz L,Kallenbach RL,Schrick FN,Waller JC

更新日期：2014-01-01 00:00:00

abstract：:P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...

journal_title：Animal biotechnology

authors： He X,Li B,Fu S,Wang B,Qi Y,Da L,Te R,Sun S,Liu Y,Zhang W

更新日期：2019-07-18 00:00:00

abstract：:In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

journal_title：Animal biotechnology

authors： Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

更新日期：2014-01-01 00:00:00