Abstract:
:The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose depots and to evaluate the tissue-dependent nature of expression reported in other species. A 438 bp fragment representing the coding region of the bovine leptin gene excluding the N-terminal secretory signal was amplified, cloned into a plasmid vector (pASK75), and expressed in E. coli. Sequence analysis of the cDNA and the corresponding polypeptide indicate that, overall, both share approximately 87% homology with the mouse and human leptin genes and polypeptides. Amino terminal sequencing (30 amino acid residues) of the recombinant bovine leptin (rBL) protein revealed 100% homology with mouse and human leptin. The bovine leptin gene is expressed as a 3,090 nt mRNA which is detected in adipose tissue, but is not found in brain (despite the appreciable fat content and lipid metabolism) or other tissues. Leptin gene expression in several adipose depots (subcutaneous, renal, and omental) was similar (P = .73) in finished cattle.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Ji S,Willis GM,Scott RR,Spurlock MEdoi
10.1080/10495399809525887subject
Has Abstractpub_date
1998-01-01 00:00:00pages
1-14issue
1eissn
1049-5398issn
1532-2378journal_volume
9pub_type
杂志文章abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500264896
更新日期:2005-01-01 00:00:00
abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1135807
更新日期:2016-01-01 00:00:00
abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
更新日期:2008-01-01 00:00:00
abstract::Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1811294
更新日期:2020-08-31 00:00:00
abstract::Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390600962274
更新日期:2006-01-01 00:00:00
abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2014.881369
更新日期:2015-01-01 00:00:00
abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437046
更新日期:2019-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章,评审
doi:10.1080/10495398.2013.870075
更新日期:2014-01-01 00:00:00
abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
更新日期:2012-04-01 00:00:00
abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802212445
更新日期:2008-01-01 00:00:00
abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1803892
更新日期:2020-08-13 00:00:00
abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120022136
更新日期:2003-05-01 00:00:00
abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1777149
更新日期:2020-06-10 00:00:00
abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-200053398
更新日期:2005-01-01 00:00:00
abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
更新日期:1998-01-01 00:00:00
abstract::This study elucidated the molecular markers that decrease oocyte quality during in vitro culture, restricting optimal developmental potential. Here, we evaluated the transcriptomic differences between cysteamine-treated and non-treated bovine cumulus oocyte complexes (COCs) after 22 h of co-culture in the maturation m...
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pub_type: 杂志文章
doi:10.1080/10495398.2019.1706545
更新日期:2020-01-03 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390801887361
更新日期:2008-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2013.839949
更新日期:2014-01-01 00:00:00
abstract::Retinoid X receptor α (RXRα) is thought to be a key regulator in lipid metabolism, glucose metabolism, adipogenic differentiation, gene expression, and inflammatory response. However, it is not clear whether RXRα has any role in intramuscular preadipocyte of goat. In the current investigation, we report that adenoviru...
journal_title:Animal biotechnology
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doi:10.1080/10495398.2020.1732397
更新日期:2020-02-27 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1612757
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1182540
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2020.1740240
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abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1329150
更新日期:2018-04-03 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1198796
更新日期:2017-01-02 00:00:00
abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1271805
更新日期:2017-10-02 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1157081
更新日期:2016-01-01 00:00:00
abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601088657
更新日期:2007-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120005779
更新日期:2002-05-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1074915
更新日期:2016-01-01 00:00:00
abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-100102977
更新日期:2001-05-01 00:00:00