WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells.

Abstract:

:In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFalpha-NFkappaB signal pathway, is associated with HC11 cell apoptosis.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Kho Y,Kim S,Yoon BS,Moon JH,Kwak S,Park G,Woo J,Oh S,Hong K,Kim S,Kim H,You S,Choi Y

doi

10.1080/10495390801887361

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

89-103

issue

2

eissn

1049-5398

issn

1532-2378

pii

792376882

journal_volume

19

pub_type

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