The effect of retroviral vector on uptake of human lactoferrin DNA by Yak (Bos Grunniens) spermatozoa and their fertilizability in vitro.

Abstract:

:The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using PLNCX2-hLF+FuGene 6 to generate SMGT yak spermatozoa for fertilizing bovine oocytes. The result showed that DNA-binding rate increased with the extension of incubation period and DNA treatment did not decrease sperm motility. Oocytes inseminated with SMGT-spermatozoa had a lower (P < 0.05) cleavage rate (57.7%) than the control (73.4%), but development up to blastocyst stage was not different (26.8 vs. 31.7%). It appears that PLNCX2 is useful for generating transgenic yaks by SMGT.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Zi XD,Chen SW,Liang GN,Chen DW,Zhang DW,Yin RH

doi

10.1080/10495390903196455

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

247-51

issue

4

eissn

1049-5398

issn

1532-2378

pii

915549490

journal_volume

20

pub_type

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