Abstract:
:The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using PLNCX2-hLF+FuGene 6 to generate SMGT yak spermatozoa for fertilizing bovine oocytes. The result showed that DNA-binding rate increased with the extension of incubation period and DNA treatment did not decrease sperm motility. Oocytes inseminated with SMGT-spermatozoa had a lower (P < 0.05) cleavage rate (57.7%) than the control (73.4%), but development up to blastocyst stage was not different (26.8 vs. 31.7%). It appears that PLNCX2 is useful for generating transgenic yaks by SMGT.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Zi XD,Chen SW,Liang GN,Chen DW,Zhang DW,Yin RHdoi
10.1080/10495390903196455subject
Has Abstractpub_date
2009-01-01 00:00:00pages
247-51issue
4eissn
1049-5398issn
1532-2378pii
915549490journal_volume
20pub_type
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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