Novel polymorphism of HSP70 gene affected caprine physiological vital parameters.


:The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar) which differ in body size, coat color and production performance were assessed for effect of thermal stress at different temperature humidity index (THI). The physiological vital parameters like rectal temperature (RT), skin temperature (ST), heart rate (HR) and respiration rate (RR) which are related to thermal stress susceptibility were recorded. The genetic variation of HSP70 gene in the three goat populations and its effect on physiological vital parameters related to thermal stress was assessed. A novel polymorphism (C241T) in the exonic region of HSP70 gene with significant (p < .05) statistical association with the physiological vital parameters like RT, ST and RR was identified indicating its role in thermo-tolerance.HighlightsThe HSP70 gene was found to be polymorphic in Indian goats.The non-synonymous nucleotide change (C241T) resulted change in amino acid Arginine 241 Cysteine in mature polypeptide which were not reported earlier.The constructed phylogenetic tree showed that Ganjam and Raighar goats are more close to each other.


Anim Biotechnol


Animal biotechnology


Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G




Has Abstract


2020-02-12 00:00:00








  • Molecular characterization and different expression patterns of the muscle ankyrin repeat protein (MARP) family during porcine skeletal muscle development in vitro and in vivo.

    abstract::CARP, ANKRD2, and DARP belong to the ankyrin repeat protein (MARP) family and play a critical role in the integration of cytoskeletal architecture, stress response, and transcriptional regulation. In this study, we cloned the cDNA and promoter sequences of porcine ankyrin repeat protein (MARP) gene family. RT-PCR anal...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Wang L,Lei M,Xiong Y

    更新日期:2011-04-01 00:00:00

  • Genetic variation at the MHC in a population of introduced wild turkeys.

    abstract::Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Bauer MM,Miller MM,Briles WE,Reed KM

    更新日期:2013-01-01 00:00:00

  • Investigation of tissue-specific human orthologous alternative splice events in pig.

    abstract::Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Nygard AB,Jørgensen CB,Cirera S,Fredholm M

    更新日期:2010-10-01 00:00:00

  • Partial cloning and expression of the bovine leptin gene.

    abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Ji S,Willis GM,Scott RR,Spurlock ME

    更新日期:1998-01-01 00:00:00

  • Determination of γ-D-glutamyl-meso-diaminopimelic acid in rumen fluid of dairy cows by pre-column chiral derivatization-HPLC.

    abstract::High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Wang Y,Gao Q,Cheng X,Chang G,Roy AC,Shen X

    更新日期:2021-01-24 00:00:00

  • A novel 17 bp InDel polymorphism within the PPARGC1A gene is significantly associated with growth traits in sheep.

    abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

    更新日期:2020-08-09 00:00:00

  • Identification of sequence tagged sites in the Asian and African elephant.

    abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Burk NE,Messer LA,Ernst CW,Rothschild MF

    更新日期:1998-01-01 00:00:00

  • Comparative pharmacokinetic study of two kinds of altrenogest oral solutions for sows.

    abstract::This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Li Y,Yang H,Xia L,Wang S,Bu S

    更新日期:2020-03-17 00:00:00

  • Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs.

    abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

    更新日期:2015-01-01 00:00:00

  • Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats.

    abstract::The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Chu MX,Jiao CL,He YQ,Wang JY,Liu ZH,Chen GH

    更新日期:2007-01-01 00:00:00

  • Genomic organization and promoter analysis of the bovine ADAM12 gene.

    abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

    更新日期:2008-01-01 00:00:00

  • A single nucleotide polymorphism in the dopamine receptor D2 gene may be informative for resistance to fescue toxicosis in angus-based cattle.

    abstract::Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Campbell BT,Kojima CJ,Cooper TA,Bastin BC,Wojakiewicz L,Kallenbach RL,Schrick FN,Waller JC

    更新日期:2014-01-01 00:00:00

  • Differential Transcriptome Analysis of Early Postnatal Developing Longissimus Dorsi Muscle from Two Pig Breeds Characterized in Divergent Myofiber Traits and Fatness.

    abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

    更新日期:2019-01-01 00:00:00

  • Transcriptome signature of two lactation stages in Ghezel sheep identifies using RNA-Sequencing.

    abstract::The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Farhadian M,Rafat SA,Panahi B,Ebrahimie E

    更新日期:2020-07-07 00:00:00

  • Identification of differentially expressed genes associated with osteochondrosis in standardbred horses using RNA arbitrarily primed PCR.

    abstract::The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Austbø L,Røed KH,Dolvik NI,Skretting G

    更新日期:2010-04-01 00:00:00

  • Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines.

    abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Gupta T,Rawat M,Kumar B,Varshney R,Chakravarti S,Qureshi S

    更新日期:2020-04-02 00:00:00

  • Cytoskeleton Genes Expression and Survival Rate Comparison Between Immature and Mature Yak Oocyte After OPS Vitrification.

    abstract::This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

    更新日期:2018-01-01 00:00:00

  • Candidate gene approach: potentional association of caspase-1, inhibitor of apoptosis protein-1, and prosaposin gene polymorphisms with response to Salmonella enteritidis challenge or vaccination in young chicks.

    abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Liu W,Lamont SJ

    更新日期:2003-05-01 00:00:00

  • Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle.

    abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

    更新日期:2011-01-01 00:00:00

  • Harvesting the genomic promise: recombineering sequences for phenotypes.

    abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Rogatcheva MB,Rund LA,Beever JE,Schook LB

    更新日期:2003-11-01 00:00:00

  • Comparison of Copy Number of HSF Genes in Two Buffalo Genomes.

    abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Lal SV,Mukherjee A,Brahma B,Gohain M,Patra MC,Saini SK,Mishra P,Ahlawat S,Upadhyaya RC,Datta TK,De S

    更新日期:2016-01-01 00:00:00

  • Maternal origin and genetic diversity of Algerian domestic chicken (Gallus gallus domesticus) from North-Western Africa based on mitochondrial DNA analysis.

    abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

    更新日期:2020-08-13 00:00:00

  • Biomedical applications of ovarian transvaginal ultrasonography in cattle.

    abstract::Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...

    journal_title:Animal biotechnology

    pub_type: 杂志文章,评审


    authors: Velazquez MA,Kues WA,Niemann H

    更新日期:2014-01-01 00:00:00

  • Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case-control study in the Indian cattle population.

    abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

    更新日期:2020-09-28 00:00:00

  • Sequence of GDF 8 (myostatin) gene in Bubalus bubalis.

    abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Tantia MS,Vijh RK,Mishra B,Kumar ST

    更新日期:2007-01-01 00:00:00

  • Metabolome fingerprints, performance and carcass quality of beef calves supplemented with antibiotic free additive.

    abstract::The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Gandra JR,Pedrini CA,Cônsolo NRB,Acosta AP,Seno LO,Barbosa LCGS,Noia IZ,Buarque VLM,Padilla ARH,Colnago LA,Gandra ERS

    更新日期:2020-10-06 00:00:00

  • Construction of a CRISPR-Cas9 System for Pig Genome Targeting.

    abstract::A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

    更新日期:2015-01-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Lipopolysaccharide binding protein/CD14/ TLR4-dependent recognition of salmonella LPS induces the functional activation of chicken heterophils and up-regulation of pro-inflammatory cytokine and chemokine gene expression in these cells.

    abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Kogut MH,He H,Kaiser P

    更新日期:2005-01-01 00:00:00

  • Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing.

    abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

    更新日期:2001-11-01 00:00:00