Novel polymorphism of HSP70 gene affected caprine physiological vital parameters.


:The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar) which differ in body size, coat color and production performance were assessed for effect of thermal stress at different temperature humidity index (THI). The physiological vital parameters like rectal temperature (RT), skin temperature (ST), heart rate (HR) and respiration rate (RR) which are related to thermal stress susceptibility were recorded. The genetic variation of HSP70 gene in the three goat populations and its effect on physiological vital parameters related to thermal stress was assessed. A novel polymorphism (C241T) in the exonic region of HSP70 gene with significant (p < .05) statistical association with the physiological vital parameters like RT, ST and RR was identified indicating its role in thermo-tolerance.HighlightsThe HSP70 gene was found to be polymorphic in Indian goats.The non-synonymous nucleotide change (C241T) resulted change in amino acid Arginine 241 Cysteine in mature polypeptide which were not reported earlier.The constructed phylogenetic tree showed that Ganjam and Raighar goats are more close to each other.


Anim Biotechnol


Animal biotechnology


Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G




Has Abstract


2020-02-12 00:00:00








  • Association of BF, RBP4, and ESR2 Genotypes with Litter Size in an Autochthonous Pig Population.

    abstract::The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Laliotis GP,Marantidis A,Avdi M

    更新日期:2017-04-03 00:00:00

  • A 3800 gene microarray for cattle functional genomics: comparison of gene expression in spleen, placenta, and brain.

    abstract::A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

    更新日期:2002-05-01 00:00:00

  • Polymorphic Microsatellite Markers for Solen grandis and Their Cross-Species Amplification in Three Other Species.

    abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

    更新日期:2019-01-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Biomedical applications of ovarian transvaginal ultrasonography in cattle.

    abstract::Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...

    journal_title:Animal biotechnology

    pub_type: 杂志文章,评审


    authors: Velazquez MA,Kues WA,Niemann H

    更新日期:2014-01-01 00:00:00

  • Establishment and characterization of proliferating primary cultures of equine epidermal keratinocytes.

    abstract::Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Ogorevc J,Poklukar K,Dovč P

    更新日期:2019-11-18 00:00:00

  • Evaluation of Bovine High-Density SNP Genotyping Array in Indigenous Dairy Cattle Breeds.

    abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Dash S,Singh A,Bhatia AK,Jayakumar S,Sharma A,Singh S,Ganguly I,Dixit SP

    更新日期:2018-04-03 00:00:00

  • A single nucleotide polymorphism in the dopamine receptor D2 gene may be informative for resistance to fescue toxicosis in angus-based cattle.

    abstract::Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Campbell BT,Kojima CJ,Cooper TA,Bastin BC,Wojakiewicz L,Kallenbach RL,Schrick FN,Waller JC

    更新日期:2014-01-01 00:00:00

  • Bovine Sex Determining Region Y: Cloning, Optimized Expression, and Purification.

    abstract::Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

    更新日期:2017-01-02 00:00:00

  • Transcriptome signature of two lactation stages in Ghezel sheep identifies using RNA-Sequencing.

    abstract::The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Farhadian M,Rafat SA,Panahi B,Ebrahimie E

    更新日期:2020-07-07 00:00:00

  • Temporal microRNA expression profile of pig peripheral blood during postnatal development.

    abstract::Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

    更新日期:2021-01-18 00:00:00

  • The expression and regulation of miR-1 in goat skeletal muscle and satellite cell during muscle growth and development.

    abstract::MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

    更新日期:2020-10-01 00:00:00

  • Genetic Variants in ADD1 Gene and their Associations with Growth Traits in Cattle.

    abstract::The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Huang YZ,Qian LN,Wang J,Zhang CL,Fang XT,Lei CZ,Lan XY,Ma Y,Bai YY,Lin FP,Chen H

    更新日期:2019-01-01 00:00:00

  • Alternative splicing of testis-specific lactate dehydrogenase C gene in mammals and pigeon.

    abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

    更新日期:2012-04-01 00:00:00

  • Molecular Characterization of Equine APRIL and its Expression Analysis During the Adipogenic Differentiation of Equine Adipose-Derived Stem Cell In Vitro.

    abstract::A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Wu H,Bi X,Cao F,Zhu C,Liu H,Song J,Ma L,Ma L,Zhang Y,Zhao D,Liu H,Xu X,Zhang S

    更新日期:2016-10-01 00:00:00

  • Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle.

    abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

    更新日期:2011-01-01 00:00:00

  • Use of touch-down polymerase chain reaction to enhance the sensitivity of Brucella melitensis detection in raw milk.

    abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Rahimi H,Tukmechi A,Rashidian E

    更新日期:2020-06-10 00:00:00

  • Cloning of bovine pyruvate carboxylase and 5' untranslated region variants.

    abstract::Bovine pyruvate carboxylase (PC; EC cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Agca C,Bidwell CA,Donkin SS

    更新日期:2004-05-01 00:00:00

  • Somatostatin can alter fertility genes expression, oocytes maturation, and embryo development in cattle.

    abstract::This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-P...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Moaeen-ud-Din M,Malik N,Yang LG

    更新日期:2009-01-01 00:00:00

  • Expressional and Bioinformatic Analysis of Bovine Filia/Ecat1/Khdc3l Gene: A Comparison with Ovine Species.

    abstract::Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

    更新日期:2016-01-01 00:00:00

  • Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case-control study in the Indian cattle population.

    abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

    更新日期:2020-09-28 00:00:00

  • Development and Utilization of VHH Antibodies Derived from Camelus Dromedarius Against Foot-and-Mouth Disease Virus.

    abstract::Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

    更新日期:2019-01-01 00:00:00

  • Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs.

    abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

    更新日期:2015-01-01 00:00:00

  • Association of the porcine transforming growth factor beta type I receptor (TGFBR1) gene with growth and carcass traits.

    abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

    更新日期:2012-01-01 00:00:00

  • Partial cloning and expression of the bovine leptin gene.

    abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Ji S,Willis GM,Scott RR,Spurlock ME

    更新日期:1998-01-01 00:00:00

  • Investigation of tissue-specific human orthologous alternative splice events in pig.

    abstract::Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Nygard AB,Jørgensen CB,Cirera S,Fredholm M

    更新日期:2010-10-01 00:00:00

  • Genetic variation at the MHC in a population of introduced wild turkeys.

    abstract::Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Bauer MM,Miller MM,Briles WE,Reed KM

    更新日期:2013-01-01 00:00:00

  • Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats.

    abstract::The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Chu MX,Jiao CL,He YQ,Wang JY,Liu ZH,Chen GH

    更新日期:2007-01-01 00:00:00

  • Effect of in-straw thawing on in vitro- and in vivo-development of vitrified mouse morulae.

    abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

    更新日期:2007-01-01 00:00:00

  • Identification of sequence tagged sites in the Asian and African elephant.

    abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章


    authors: Burk NE,Messer LA,Ernst CW,Rothschild MF

    更新日期:1998-01-01 00:00:00