Novel polymorphism of HSP70 gene affected caprine physiological vital parameters.

abstract：:The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...

journal_title：Animal biotechnology

authors： Laliotis GP,Marantidis A,Avdi M

更新日期：2017-04-03 00:00:00

abstract：:A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

journal_title：Animal biotechnology

authors： Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

更新日期：2002-05-01 00:00:00

abstract：:The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

journal_title：Animal biotechnology

authors： Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

更新日期：2019-01-01 00:00:00

abstract：:The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

journal_title：Animal biotechnology

authors： Fries R,Ewald D,Thaller G,Buitkamp J

更新日期：2001-05-01 00:00:00

abstract：:Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...

journal_title：Animal biotechnology

authors： Velazquez MA,Kues WA,Niemann H

更新日期：2014-01-01 00:00:00

abstract：:Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

journal_title：Animal biotechnology

authors： Ogorevc J,Poklukar K,Dovč P

更新日期：2019-11-18 00:00:00

abstract：:In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...

journal_title：Animal biotechnology

authors： Dash S,Singh A,Bhatia AK,Jayakumar S,Sharma A,Singh S,Ganguly I,Dixit SP

更新日期：2018-04-03 00:00:00

abstract：:Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...

journal_title：Animal biotechnology

authors： Campbell BT,Kojima CJ,Cooper TA,Bastin BC,Wojakiewicz L,Kallenbach RL,Schrick FN,Waller JC

更新日期：2014-01-01 00:00:00

abstract：:Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

journal_title：Animal biotechnology

authors： Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

更新日期：2017-01-02 00:00:00

abstract：:The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...

journal_title：Animal biotechnology

authors： Farhadian M,Rafat SA,Panahi B,Ebrahimie E

更新日期：2020-07-07 00:00:00

abstract：:Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

journal_title：Animal biotechnology

authors： Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

更新日期：2021-01-18 00:00:00

abstract：:MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

journal_title：Animal biotechnology

authors： Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

更新日期：2020-10-01 00:00:00

abstract：:The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...

journal_title：Animal biotechnology

authors： Huang YZ,Qian LN,Wang J,Zhang CL,Fang XT,Lei CZ,Lan XY,Ma Y,Bai YY,Lin FP,Chen H

更新日期：2019-01-01 00:00:00

abstract：:The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...

journal_title：Animal biotechnology

authors： Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

更新日期：2012-04-01 00:00:00

abstract：:A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...

journal_title：Animal biotechnology

authors： Wu H,Bi X,Cao F,Zhu C,Liu H,Song J,Ma L,Ma L,Zhang Y,Zhao D,Liu H,Xu X,Zhang S

更新日期：2016-10-01 00:00:00

abstract：:We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

journal_title：Animal biotechnology

authors： Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

更新日期：2011-01-01 00:00:00

abstract：:Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...

journal_title：Animal biotechnology

authors： Rahimi H,Tukmechi A,Rashidian E

更新日期：2020-06-10 00:00:00

abstract：:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

journal_title：Animal biotechnology

authors： Agca C,Bidwell CA,Donkin SS

更新日期：2004-05-01 00:00:00

abstract：:This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-P...

journal_title：Animal biotechnology

authors： Moaeen-ud-Din M,Malik N,Yang LG

更新日期：2009-01-01 00:00:00

abstract：:Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

journal_title：Animal biotechnology

authors： Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

更新日期：2016-01-01 00:00:00

abstract：:This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

journal_title：Animal biotechnology

authors： Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

更新日期：2020-09-28 00:00:00

abstract：:Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

journal_title：Animal biotechnology

authors： Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

更新日期：2019-01-01 00:00:00

abstract：:The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...

journal_title：Animal biotechnology

authors： Chen K,Hawken R,Flickinger GH,Rodriguez-Zas SL,Rund LA,Wheeler MB,Abrahamsen M,Rutherford MS,Beever JE,Schook LB

更新日期：2012-01-01 00:00:00

abstract：:The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

journal_title：Animal biotechnology

authors： Ji S,Willis GM,Scott RR,Spurlock ME

更新日期：1998-01-01 00:00:00

abstract：:Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

journal_title：Animal biotechnology

authors： Nygard AB,Jørgensen CB,Cirera S,Fredholm M

更新日期：2010-10-01 00:00:00

abstract：:Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

journal_title：Animal biotechnology

authors： Bauer MM,Miller MM,Briles WE,Reed KM

更新日期：2013-01-01 00:00:00

abstract：:The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...

journal_title：Animal biotechnology

authors： Chu MX,Jiao CL,He YQ,Wang JY,Liu ZH,Chen GH

更新日期：2007-01-01 00:00:00

abstract：:For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

journal_title：Animal biotechnology

authors： Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

更新日期：2007-01-01 00:00:00

abstract：:To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

journal_title：Animal biotechnology

authors： Burk NE,Messer LA,Ernst CW,Rothschild MF

更新日期：1998-01-01 00:00:00