Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen.

abstract：:A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

journal_title：Animal biotechnology

authors： Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

更新日期：2015-01-01 00:00:00

abstract：:Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...

journal_title：Animal biotechnology

authors： Boudali SF,Al-Jumaili AS,Bouandas A,Mahammi FZ,Tabet Aoul N,Hanotte O,Gaouar SBS

更新日期：2020-08-13 00:00:00

abstract：:The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...

journal_title：Animal biotechnology

authors： Awotunde EO,Bemji MN,Olowofeso O,James IJ,Ajayi OO,Adebambo AO

更新日期：2015-01-01 00:00:00

abstract：:The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...

journal_title：Animal biotechnology

authors： Chu MX,Jiao CL,He YQ,Wang JY,Liu ZH,Chen GH

更新日期：2007-01-01 00:00:00

abstract：:Aim of this study was to compare different combinations of penetrating intracellular CPAs, i.e., glycerol (G), ethylene glycol (EG), propylene glycol (PG), dimethyl formamide (DM), and methyl acetamide (MA) and extracellular [egg yolk (EY), egg yolk plasma (EYP), low-density lipoproteins (LDL), and coconut water (CW)]...

journal_title：Animal biotechnology

authors： Cheema RS,Kaur S,Mavi GK,Singh AK,Honparkhe M,Gandotra VK

更新日期：2019-12-08 00:00:00

abstract：:Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

journal_title：Animal biotechnology

authors： Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

更新日期：2005-01-01 00:00:00

abstract：:The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

journal_title：Animal biotechnology

authors： Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

更新日期：2020-07-31 00:00:00

abstract：:Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

journal_title：Animal biotechnology

authors： Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

更新日期：2016-01-01 00:00:00

abstract：:CARP, ANKRD2, and DARP belong to the ankyrin repeat protein (MARP) family and play a critical role in the integration of cytoskeletal architecture, stress response, and transcriptional regulation. In this study, we cloned the cDNA and promoter sequences of porcine ankyrin repeat protein (MARP) gene family. RT-PCR anal...

journal_title：Animal biotechnology

authors： Wang L,Lei M,Xiong Y

更新日期：2011-04-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

journal_title：Animal biotechnology

authors： Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

更新日期：2019-01-01 00:00:00

abstract：:Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternati...

journal_title：Animal biotechnology

authors： Nygard AB,Jørgensen CB,Cirera S,Fredholm M

更新日期：2010-10-01 00:00:00

abstract：:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...

journal_title：Animal biotechnology

authors： Yonekura S,Hirota S,Miyazaki H,Tokutake Y

更新日期：2016-01-01 00:00:00

abstract：:This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

journal_title：Animal biotechnology

authors： Li Y,Yang H,Xia L,Wang S,Bu S

更新日期：2020-03-17 00:00:00

abstract：:Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

journal_title：Animal biotechnology

authors： Ogorevc J,Poklukar K,Dovč P

更新日期：2019-11-18 00:00:00

abstract：:Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

journal_title：Animal biotechnology

authors： Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

更新日期：2018-04-03 00:00:00

abstract：:Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

journal_title：Animal biotechnology

authors： Salem M,Nath J,Killefer J

更新日期：2004-01-01 00:00:00

abstract：:Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...

journal_title：Animal biotechnology

authors： Velazquez MA,Kues WA,Niemann H

更新日期：2014-01-01 00:00:00

abstract：:The α-adducin (ADD1) is a subunit of adducin which is a cytoskeleton heterodimeric protein. Adducin participates in oocytes chromosome meiosis of mice, prompting adducin has an effect on embryonic development. Adducin gene mutation has significantly functional change. So the present study was to identify and character...

journal_title：Animal biotechnology

authors： Huang YZ,Qian LN,Wang J,Zhang CL,Fang XT,Lei CZ,Lan XY,Ma Y,Bai YY,Lin FP,Chen H

更新日期：2019-01-01 00:00:00

abstract：:MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

journal_title：Animal biotechnology

authors： Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

更新日期：2020-10-01 00:00:00

abstract：:The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

journal_title：Animal biotechnology

authors： Rogatcheva MB,Rund LA,Beever JE,Schook LB

更新日期：2003-11-01 00:00:00

abstract：:The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

journal_title：Animal biotechnology

authors： Tantia MS,Vijh RK,Mishra B,Kumar ST

更新日期：2007-01-01 00:00:00

abstract：:The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

journal_title：Animal biotechnology

authors： Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

更新日期：2017-10-02 00:00:00

abstract：:We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

journal_title：Animal biotechnology

authors： Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

更新日期：2011-01-01 00:00:00

abstract：:Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

journal_title：Animal biotechnology

authors： Bauer MM,Miller MM,Briles WE,Reed KM

更新日期：2013-01-01 00:00:00

abstract：:In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Dall'Olio S,Buttazzoni L,Gallo M,Russo V

更新日期：2012-01-01 00:00:00

abstract：:A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and th...

journal_title：Animal biotechnology

authors： Wu H,Bi X,Cao F,Zhu C,Liu H,Song J,Ma L,Ma L,Zhang Y,Zhao D,Liu H,Xu X,Zhang S

更新日期：2016-10-01 00:00:00

abstract：:Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...

journal_title：Animal biotechnology

authors： Soleymani B,Hafezian SH,Mianji GR,Mansouri K,Chaharaein B,Tajehmiri A,Sharifi Tabar M,Mostafaie A

更新日期：2017-01-02 00:00:00

abstract：:The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

journal_title：Animal biotechnology

authors： Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

更新日期：2013-01-01 00:00:00

abstract：:The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

journal_title：Animal biotechnology

authors： Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

更新日期：2019-01-01 00:00:00

abstract：:The aim of this study was to investigate genes for differential expression in cartilage of foals predisposed to osteochondrosis (OC). Tissue was sampled from the cranial part of the distal intermediate ridge of the tibia in the tarso-crural joint. Foals were considered predisposed to OC when parents had OC at the dist...

journal_title：Animal biotechnology

authors： Austbø L,Røed KH,Dolvik NI,Skretting G

更新日期：2010-04-01 00:00:00