Abstract:
:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP4 polymorphism leads to different palmitoleic acid contents. We overexpressed FABP4 of different genotypes in bovine intramuscular preadipocytes and examined whether the intracellular localization of FABP4 and the expression levels of lipid metabolism-related genes were different among cells expressing different genotypes. Nuclear localization was observed for the FABP4 V/V, while the FABP4 I/I almost did not. The cells expressing FABP4 of different genotypes were comparable in terms of the expression levels of genes involved in lipid metabolism. FABP4 I/I was localized in most of the lipid droplets 4 days after differentiation induction, whereas approximately 25% lipid droplet co-localized with FABP4 in cells expressing FABP4 V/V. The lipid droplet size increased when palmitoleic acid was added compared to the size observed when palmitic acid was added. These results suggest that lipid droplet enlargement caused by palmitoleic acid and genotype-dependent differences in the fatty acid transporting capacity underlie variations in palmitoleic acid content among FABP4 polymorphisms.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Yonekura S,Hirota S,Miyazaki H,Tokutake Ydoi
10.1080/10495398.2015.1102148subject
Has Abstractpub_date
2016-01-01 00:00:00pages
96-103issue
2eissn
1049-5398issn
1532-2378journal_volume
27pub_type
杂志文章abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525921
更新日期:1999-01-01 00:00:00
abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601091040
更新日期:2007-01-01 00:00:00
abstract::The pig is a recognized model for the onset of coronary heart disease and heart attacks. Previous studies have shown that serum cholesterol levels in the pig can be elevated using a high fat, high cholesterol (HFHC) diet. What has been lacking is a genetically defined model corresponding to human ApoE4 susceptibility ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2010.490693
更新日期:2010-07-01 00:00:00
abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500264896
更新日期:2005-01-01 00:00:00
abstract::This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1369429
更新日期:2018-01-01 00:00:00
abstract::Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1347881
更新日期:2018-04-03 00:00:00
abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1735404
更新日期:2020-04-02 00:00:00
abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1863225
更新日期:2021-01-10 00:00:00
abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1367690
更新日期:2019-01-01 00:00:00
abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120026481
更新日期:2003-11-01 00:00:00
abstract::This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1723608
更新日期:2020-03-17 00:00:00
abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-200053398
更新日期:2005-01-01 00:00:00
abstract::The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1726364
更新日期:2020-02-12 00:00:00
abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-100108341
更新日期:2001-11-01 00:00:00
abstract::Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1740240
更新日期:2020-03-17 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1796697
更新日期:2020-08-09 00:00:00
abstract::Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390600962274
更新日期:2006-01-01 00:00:00
abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1271805
更新日期:2017-10-02 00:00:00
abstract::Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1824920
更新日期:2021-01-18 00:00:00
abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390802340923
更新日期:2008-01-01 00:00:00
abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1313269
更新日期:2018-04-03 00:00:00
abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437045
更新日期:2019-01-01 00:00:00
abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601115526
更新日期:2007-01-01 00:00:00
abstract::Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/LABT-200036706
更新日期:2004-01-01 00:00:00
abstract::The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2014.977907
更新日期:2015-01-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1433191
更新日期:2019-01-01 00:00:00
abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437046
更新日期:2019-01-01 00:00:00
abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-100102977
更新日期:2001-05-01 00:00:00
abstract::P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1640717
更新日期:2019-07-18 00:00:00
abstract::The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group th...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1828095
更新日期:2020-10-06 00:00:00