Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.

Abstract:

:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP4 polymorphism leads to different palmitoleic acid contents. We overexpressed FABP4 of different genotypes in bovine intramuscular preadipocytes and examined whether the intracellular localization of FABP4 and the expression levels of lipid metabolism-related genes were different among cells expressing different genotypes. Nuclear localization was observed for the FABP4 V/V, while the FABP4 I/I almost did not. The cells expressing FABP4 of different genotypes were comparable in terms of the expression levels of genes involved in lipid metabolism. FABP4 I/I was localized in most of the lipid droplets 4 days after differentiation induction, whereas approximately 25% lipid droplet co-localized with FABP4 in cells expressing FABP4 V/V. The lipid droplet size increased when palmitoleic acid was added compared to the size observed when palmitic acid was added. These results suggest that lipid droplet enlargement caused by palmitoleic acid and genotype-dependent differences in the fatty acid transporting capacity underlie variations in palmitoleic acid content among FABP4 polymorphisms.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Yonekura S,Hirota S,Miyazaki H,Tokutake Y

doi

10.1080/10495398.2015.1102148

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

96-103

issue

2

eissn

1049-5398

issn

1532-2378

journal_volume

27

pub_type

杂志文章
  • SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1).

    abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399909525921

    authors: Prinzenberg EM,Krause I,Erhardt G

    更新日期:1999-01-01 00:00:00

  • Effect of in-straw thawing on in vitro- and in vivo-development of vitrified mouse morulae.

    abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601091040

    authors: Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

    更新日期:2007-01-01 00:00:00

  • A cloned pig model for examining atherosclerosis induced by high fat, high cholesterol diets.

    abstract::The pig is a recognized model for the onset of coronary heart disease and heart attacks. Previous studies have shown that serum cholesterol levels in the pig can be elevated using a high fat, high cholesterol (HFHC) diet. What has been lacking is a genetically defined model corresponding to human ApoE4 susceptibility ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2010.490693

    authors: Jensen TW,Mazur MJ,Pettigew JE,Perez-Mendoza VG,Zachary J,Schook LB

    更新日期:2010-07-01 00:00:00

  • Lipopolysaccharide binding protein/CD14/ TLR4-dependent recognition of salmonella LPS induces the functional activation of chicken heterophils and up-regulation of pro-inflammatory cytokine and chemokine gene expression in these cells.

    abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390500264896

    authors: Kogut MH,He H,Kaiser P

    更新日期:2005-01-01 00:00:00

  • Cytoskeleton Genes Expression and Survival Rate Comparison Between Immature and Mature Yak Oocyte After OPS Vitrification.

    abstract::This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1369429

    authors: Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

    更新日期:2018-01-01 00:00:00

  • Mutations in Leptin (LEP) Gene Are Associated with Carcass and Meat Quality Traits in Crossbreed Rabbits.

    abstract::Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1347881

    authors: Migdal L,Koziol K,Palka S,Migdal W,Zabek T,Otwinowska-Mindur A,Migdal A,Kmiecik M,Maj D,Bieniek J

    更新日期:2018-04-03 00:00:00

  • Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines.

    abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1735404

    authors: Gupta T,Rawat M,Kumar B,Varshney R,Chakravarti S,Qureshi S

    更新日期:2020-04-02 00:00:00

  • Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen.

    abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1863225

    authors: Ali SA,Boby N,Preena P,Singh SV,Kaur G,Ghosh SK,Nandi S,Chaudhuri P

    更新日期:2021-01-10 00:00:00

  • Polymorphic Microsatellite Markers for Solen grandis and Their Cross-Species Amplification in Three Other Species.

    abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1367690

    authors: Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

    更新日期:2019-01-01 00:00:00

  • Harvesting the genomic promise: recombineering sequences for phenotypes.

    abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120026481

    authors: Rogatcheva MB,Rund LA,Beever JE,Schook LB

    更新日期:2003-11-01 00:00:00

  • Comparative pharmacokinetic study of two kinds of altrenogest oral solutions for sows.

    abstract::This study was to compare the pharmacokinetic characteristics of domestic altrenogest oral solution (DAOS) or imported altrenogest oral solution (IAOS) in healthy sows. A single administration (1 mg/kg body weight) of DAOS or IAOS was performed in sixteen healthy sows according to a two-period crossover design. Plasma...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1723608

    authors: Li Y,Yang H,Xia L,Wang S,Bu S

    更新日期:2020-03-17 00:00:00

  • Characterization of a normalized cDNA library from bovine intestinal muscle and epithelial tissues.

    abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-200053398

    authors: Baumann RG,Baldwin RL,Van Tassell CP 6th,Sonstegard TS,Matukumalli LK

    更新日期:2005-01-01 00:00:00

  • Novel polymorphism of HSP70 gene affected caprine physiological vital parameters.

    abstract::The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1726364

    authors: Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G

    更新日期:2020-02-12 00:00:00

  • Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing.

    abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-100108341

    authors: Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

    更新日期:2001-11-01 00:00:00

  • The specificity protein 3 (SP3) gene in ducks (Anas platyrhynchos): cloning, characterization and expression during viral infection.

    abstract::Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, includ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1740240

    authors: Cao Z,Gao W,Gu T,Huo W,Zhang Y,Zhang Y,Xu Q,Chen G

    更新日期:2020-03-17 00:00:00

  • A novel 17 bp InDel polymorphism within the PPARGC1A gene is significantly associated with growth traits in sheep.

    abstract::Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a member of transcriptional coactivator of the peroxisome proliferator-activated receptor. It is involved in lipid metabolism, energy metabolism, adipocyte differentiation and regulation of mitochondrial biogenesis. Therefore, the genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1796697

    authors: Chen P,Zhao H,Wu M,He S,Yuan T,Yi X,Liu S,Pan Y,Li Q,Wang S,Sun X

    更新日期:2020-08-09 00:00:00

  • Health management with reduced antibiotic use - the U.S. experience.

    abstract::Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390600962274

    authors: Baker R

    更新日期:2006-01-01 00:00:00

  • Combined Haplotypes of CaSR Gene Sequence Variants and Their Associations with Growth Traits in Cattle.

    abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1271805

    authors: Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

    更新日期:2017-10-02 00:00:00

  • Temporal microRNA expression profile of pig peripheral blood during postnatal development.

    abstract::Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this stu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1824920

    authors: Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv X

    更新日期:2021-01-18 00:00:00

  • Simple sequence repeats for genetic studies of alpaca.

    abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802340923

    authors: Reed KM,Chaves LD

    更新日期:2008-01-01 00:00:00

  • Effects of Recombinant Human Lactoferrin on Osteoblast Growth and Bone Status in Piglets.

    abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1313269

    authors: Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

    更新日期:2018-04-03 00:00:00

  • Differential Transcriptome Analysis of Early Postnatal Developing Longissimus Dorsi Muscle from Two Pig Breeds Characterized in Divergent Myofiber Traits and Fatness.

    abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1437045

    authors: Xu X,Mishra B,Qin N,Sun X,Zhang S,Yang J,Xu R

    更新日期:2019-01-01 00:00:00

  • Sequence of GDF 8 (myostatin) gene in Bubalus bubalis.

    abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601115526

    authors: Tantia MS,Vijh RK,Mishra B,Kumar ST

    更新日期:2007-01-01 00:00:00

  • Cloning of the calpain regulatory subunit cDNA from fish reveals a divergent domain-V.

    abstract::Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/LABT-200036706

    authors: Salem M,Nath J,Killefer J

    更新日期:2004-01-01 00:00:00

  • Mitochondrial DNA sequence analyses and phylogenetic relationships among two Nigerian goat breeds and the South African Kalahari Red.

    abstract::The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2014.977907

    authors: Awotunde EO,Bemji MN,Olowofeso O,James IJ,Ajayi OO,Adebambo AO

    更新日期:2015-01-01 00:00:00

  • Development and Utilization of VHH Antibodies Derived from Camelus Dromedarius Against Foot-and-Mouth Disease Virus.

    abstract::Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of sero...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1433191

    authors: Dash L,Subramaniam S,Khulape SA,Prusty BR,Pargai K,Narnaware SD,Patil NV,Pattnaik B

    更新日期:2019-01-01 00:00:00

  • Candidate SNP of CACNA2D1 Gene Associated with Clinical Mastitis and Production Traits in Sahiwal (Bos taurus indicus) and Karan Fries (Bos taurus taurus × Bos taurus indicus).

    abstract::The present study was conducted to identify polymorphisms in CACNA2D1 gene and their association with clinical mastitis and production traits. Exon 18 and its flanking regions were screened for the presence of SNPs. Statistical analysis was performed to identify association of period of birth, breed, and genotype with...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2018.1437046

    authors: Magotra A,Gupta ID,Verma A,Alex R,Mr V,Ahmad T

    更新日期:2019-01-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-100102977

    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Identification of piRNAs in the testes of Sunite and Small-tailed Han sheep.

    abstract::P-element-induced wimpy testis-interacting RNAs (piRNAs) are small RNAs that are essential for mammalian spermatogenesis and testicular development. Comparative analyses of the molecular mechanisms of spermatogenesis among different organisms are therefore dependent on accurate piRNA characterizations. In this study, ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2019.1640717

    authors: He X,Li B,Fu S,Wang B,Qi Y,Da L,Te R,Sun S,Liu Y,Zhang W

    更新日期:2019-07-18 00:00:00

  • Metabolome fingerprints, performance and carcass quality of beef calves supplemented with antibiotic free additive.

    abstract::The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1828095

    authors: Gandra JR,Pedrini CA,Cônsolo NRB,Acosta AP,Seno LO,Barbosa LCGS,Noia IZ,Buarque VLM,Padilla ARH,Colnago LA,Gandra ERS

    更新日期:2020-10-06 00:00:00