Effects of Recombinant Human Lactoferrin on Osteoblast Growth and Bone Status in Piglets.

Abstract:

:Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regarding the effects of recombinant human LF (rhLF) supplementation on bone status in young healthy infants. We found osteoblast cell differentiation was significantly promoted in vitro. Furthermore, treatment of human osteoblast cells with rhLF rapidly induced phosphorylation of p44/p42 mitogen-activated protein kinase (p44/p42 MAPK, ERK1/2). In order to investigate the effects of rhLF on bone status in vivo, we used a piglet model, which is a useful model for human infants. Piglets were supplemented with rhLF milk for 30 days. Bone formation markers, Serum calcium concentration, bone mineral density (BMD), bone mineral content (BMC), tibia bone strength, and the overall metabolite profile analysis showed that rhLF was advantageous to the bone growth in piglets. These findings suggest that rhLF supplementation benefits neonate bone health by modulating bone formation.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

doi

10.1080/10495398.2017.1313269

subject

Has Abstract

pub_date

2018-04-03 00:00:00

pages

90-99

issue

2

eissn

1049-5398

issn

1532-2378

journal_volume

29

pub_type

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