Biomedical applications of ovarian transvaginal ultrasonography in cattle.

abstract：:The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

journal_title：Animal biotechnology

authors： Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

更新日期：2001-11-01 00:00:00

abstract：:A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...

journal_title：Animal biotechnology

authors： Prinzenberg EM,Krause I,Erhardt G

更新日期：1999-01-01 00:00:00

abstract：:This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...

journal_title：Animal biotechnology

authors： Wei X,Sijie Y,Weibin Z,Qing X,Jie Z,Xiangdong Z

更新日期：2018-01-01 00:00:00

abstract：:Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length...

journal_title：Animal biotechnology

authors： Kumar R,Ramteke PW,Sharma SK,Mitra A

更新日期：2015-01-01 00:00:00

abstract：:The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

journal_title：Animal biotechnology

authors： Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

更新日期：2011-07-01 00:00:00

abstract：:Medicinal plants exhibit colossal impact on poultry industries by improving its performance and productivity. However, some of these plants show adverse influence too by decreasing egg production percentage, egg mass, and microbiota counts. Green tea, nettle, pennyroyal, yarrow, and alfalfa in the form of seed, powder...

journal_title：Animal biotechnology

authors： Pliego AB,Tavakoli M,Khusro A,Seidavi A,Elghandour MMMY,Salem AZM,Márquez-Molina O,Rene Rivas-Caceres R

更新日期：2020-08-03 00:00:00

abstract：:A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant pro...

journal_title：Animal biotechnology

authors： Su YH,Lin TY,Huang CL,Tu CF,Chuang CK

更新日期：2015-01-01 00:00:00

abstract：:Knockdown of myostatin gene (MSTN), transforming growth factor-β superfamily, and a negative regulator of the skeletal muscle growth, by RNA interference (RNAi), has been reported to increase muscle mass in mammals. The current study was aimed to cotransfect two anti-MSTN short hairpin RNA (shRNA) constructs in caprin...

journal_title：Animal biotechnology

authors： Hati Boruah JL,Ranjan R,Gogoi H,Pandey SK,Kumar D,Phukan AJ,Bori J,Sarkhel BC

更新日期：2016-01-01 00:00:00

abstract：:MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 ...

journal_title：Animal biotechnology

authors： Sui M,Zheng Q,Wu H,Zhu L,Ling Y,Wang L,Fang F,Liu Y,Zhang Z,Chu M,Zhang Y

更新日期：2020-10-01 00:00:00

abstract：:Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was...

journal_title：Animal biotechnology

authors： Chen X,Luo Y,Jia G,Liu G,Zhao H,Huang Z

更新日期：2017-10-02 00:00:00

abstract：:Growth arrest and DNA-damage-inducible gamma (GADD45G) is a reproduction related gene. In this study, the full-length cDNA sequence of porcine GADD45G gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine GADD45G gene encodes a protein of 159 amino acids that shares high homology with the...

journal_title：Animal biotechnology

authors： Liu C,Zhang W,Yang D,Liu Y

更新日期：2015-01-01 00:00:00

abstract：:This study was designed to investigate the effect of somatostatin on oocytes maturation and subsequent embryo development in cattle. Bovine granulosa cells separated from oocytes, cultured for 24 h and transfected with pEGFP-N1 vector with mouse SST gene (Experimental) and with out plasmid transfection (Control). RT-P...

journal_title：Animal biotechnology

authors： Moaeen-ud-Din M,Malik N,Yang LG

更新日期：2009-01-01 00:00:00

abstract：:The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

journal_title：Animal biotechnology

authors： Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

更新日期：2017-10-02 00:00:00

abstract：:The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

journal_title：Animal biotechnology

authors： Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

更新日期：2015-01-01 00:00:00

abstract：:Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...

journal_title：Animal biotechnology

authors： Li Q,Zhao J,Hu W,Wang J,Yu T,Dai Y,Li N

更新日期：2018-04-03 00:00:00

abstract：:Skin-derived tissue cultures are a useful model to study molecular mechanisms of skin renewal and pathogenesis of dermal diseases. Horses often suffer from skin diseases, skin trauma and problems with proper wound healing, which could be improved by in vitro grown keratinocyte grafts. Herein we describe establishment ...

journal_title：Animal biotechnology

authors： Ogorevc J,Poklukar K,Dovč P

更新日期：2019-11-18 00:00:00

abstract：:Indigenous cattle of India belong to the species, Bos indicus and they possess various adaptability and production traits. However, little is known about the genetic diversity and origin of these breeds. To investigate the status, we sequenced and analyzed the whole mitochondrial DNA (mtDNA) of seven Indian cattle bre...

journal_title：Animal biotechnology

authors： Pramod RK,Velayutham D,P K S,P S B,Zachariah A,Zachariah A,B C,S S S,P G,Dhinoth Kumar B,Iype S,Gupta R,Santhosh S,Thomas G

更新日期：2019-07-01 00:00:00

abstract：:Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...

journal_title：Animal biotechnology

authors： Shi X,Xia C,Pan B,Wang M

更新日期：2006-01-01 00:00:00

abstract：:Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...

journal_title：Animal biotechnology

authors： Yonekura S,Hirota S,Miyazaki H,Tokutake Y

更新日期：2016-01-01 00:00:00

abstract：:Prolactin is a versatile hormone with multiple activities, including a negative control on egg production. This study was conducted to genotype all the coding portions of the prl gene using PCR-SSCP-sequencing, and to investigate the effects of amino acid substitutions of the prl gene on the structure and function of ...

journal_title：Animal biotechnology

authors： Al-Shuhaib MBS,Al-Kafajy FR,Al-Jashami GS

更新日期：2019-10-29 00:00:00

abstract：:In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 express...

journal_title：Animal biotechnology

authors： Kho Y,Kim S,Yoon BS,Moon JH,Kwak S,Park G,Woo J,Oh S,Hong K,Kim S,Kim H,You S,Choi Y

更新日期：2008-01-01 00:00:00

abstract：:The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) s...

journal_title：Animal biotechnology

authors： Farhadian M,Rafat SA,Panahi B,Ebrahimie E

更新日期：2020-07-07 00:00:00

abstract：:Since World War II the use of antimicrobial products associated with food animal production has increased. Antimicrobials along with evolving production practices have significantly increased throughput, animal welfare, and improved health. Concerns surrounding the growing significance of emerging and in some cases ra...

journal_title：Animal biotechnology

authors： Baker R

更新日期：2006-01-01 00:00:00

abstract：:Aim of this study was to compare different combinations of penetrating intracellular CPAs, i.e., glycerol (G), ethylene glycol (EG), propylene glycol (PG), dimethyl formamide (DM), and methyl acetamide (MA) and extracellular [egg yolk (EY), egg yolk plasma (EYP), low-density lipoproteins (LDL), and coconut water (CW)]...

journal_title：Animal biotechnology

authors： Cheema RS,Kaur S,Mavi GK,Singh AK,Honparkhe M,Gandotra VK

更新日期：2019-12-08 00:00:00

abstract：:Maternal effect genes have highly impressive effects on pre-implantation development. Filia/Ecat1/Khdc3l is a maternal effect gene found in mouse oocytes and embryos, loss of which causes a 50% decrease in fertility. In the present study, we investigated Filia mRNA expression in bovine oviduct, 30- to 40-day fetus, li...

journal_title：Animal biotechnology

authors： Zahmatkesh A,Ansari Mahyari S,Daliri Joupari M,Rahmani H,Shirazi A,Amiri Roudbar M,Ansari Majd S

更新日期：2016-01-01 00:00:00

abstract：:This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

journal_title：Animal biotechnology

authors： Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

更新日期：2020-09-28 00:00:00

abstract：:The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...

journal_title：Animal biotechnology

authors： Civánová K,Knoll A

更新日期：2007-01-01 00:00:00

abstract：:Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'U...

journal_title：Animal biotechnology

authors： Agca C,Bidwell CA,Donkin SS

更新日期：2004-05-01 00:00:00

abstract：:A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

journal_title：Animal biotechnology

authors： Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

更新日期：2002-05-01 00:00:00

abstract：:The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...

journal_title：Animal biotechnology

authors： Huang L,Lin Y,Jin S,Liu W,Xu Y,Zheng Y

更新日期：2012-04-01 00:00:00