Abstract:
:A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H and 1), and the third (named CSN3*A1) was found in unrelated animals, thus indicating the presence of three additional alleles at the bovine CSN3 locus. DNA sequencing revealed single mutations that led to a Thr/Ile substitution in amino acid position 135 for CSN3*H and to a Ser/Ala substitution in position 104 of the deduced amino acid sequence of CSN3*1 (GenBank accession numbers AF105260 and AF121023) compared to CSN3*A. In CSN3*A1, a silent mutation in the third codon position of Pro150 was found (GenBank accession number AF092513).
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Prinzenberg EM,Krause I,Erhardt Gdoi
10.1080/10495399909525921keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
49-62issue
1-2eissn
1049-5398issn
1532-2378journal_volume
10pub_type
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journal_title:Animal biotechnology
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