Abstract:
:This study elucidated the molecular markers that decrease oocyte quality during in vitro culture, restricting optimal developmental potential. Here, we evaluated the transcriptomic differences between cysteamine-treated and non-treated bovine cumulus oocyte complexes (COCs) after 22 h of co-culture in the maturation media using RNA sequencing. In total, 39,014 transcripts were sequenced between cysteamine-treated and non-treated mature COCs. We evaluated the relative expression of 21,472 genes, with 59 genes showing differential expression between the two COC groups. The cysteamine-treated group had 36 up-regulated gene transcripts and 23 down-regulated gene transcripts. Moreover, gene ontology (GO) enrichment analysis revealed that multiple biological processes were significantly enriched after cysteamine supplementation. Differentially expressed genes appeared to maintain normal oocyte physiology, regulation of apoptosis, differentiation, ossification or bone formation, cardiac and muscle physiology, hormonal secretion, and membrane construction for further embryonic development. In conclusion, cysteamine affects the mRNA level of COCs during oocyte maturation by upregulating potential molecular markers and downregulating genes that affect further embryonic development.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Chowdhury MMR,Park J,Afrin F,Ko YG,Kim CL,Lee SS,Kim SWdoi
10.1080/10495398.2019.1706545subject
Has Abstractpub_date
2020-01-03 00:00:00pages
1-12eissn
1049-5398issn
1532-2378pub_type
杂志文章abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2014.881369
更新日期:2015-01-01 00:00:00
abstract::Calpains are Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed micro- and m-calpains. Both are heterodimers, consisting of a distinct catalytic subunit and a common regulatory subunit. We describe cloning and sequencing of the calpain small (regulatory) subunit (cpns) cDNA from rain...
journal_title:Animal biotechnology
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doi:10.1081/LABT-200036706
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2011.559562
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2019.1612757
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2011.652325
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1157081
更新日期:2016-01-01 00:00:00
abstract::The Jining Grey is a prolific local goat breed in P.R. China. Bone morphogenetic protein 15 (BMP15) gene that controls high fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge, and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of ovine...
journal_title:Animal biotechnology
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journal_title:Animal biotechnology
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doi:10.1080/10495399909525921
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abstract:BACKGROUND:Growth and carcass traits are of great economic importance in livestock production. A large number of quantitative trait loci (QTL) have been identified for growth and carcass traits on porcine chromosome one (SSC1). A key positional candidate for this chromosomal region is TGFBR1 (transforming growth factor...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.630897
更新日期:2012-01-01 00:00:00
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journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1005216
更新日期:2015-01-01 00:00:00
abstract::In pigs, susceptibility to enterotoxigenic Escherichia coli (ETEC) K88 strains (locus F4bcR) is determined by a dominant allele, with the recessive allele determining resistance. The susceptible allele also appeared to be associated with higher growth rate even with discordant results. A single nucleotide polymorphism...
journal_title:Animal biotechnology
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doi:10.1080/10495398.2011.653462
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abstract::Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...
journal_title:Animal biotechnology
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doi:10.1080/10495398.2015.1102148
更新日期:2016-01-01 00:00:00
abstract::Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...
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doi:10.1080/10495398.2013.796960
更新日期:2014-01-01 00:00:00
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journal_title:Animal biotechnology
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doi:10.1080/10495390009525945
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doi:10.1080/10495398.2020.1870484
更新日期:2021-01-24 00:00:00
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journal_title:Animal biotechnology
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doi:10.1080/10495398.2014.977907
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doi:10.1080/10495398.2013.872653
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journal_title:Animal biotechnology
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