Genetic characterization and assessment of diversity in Pandharpuri buffalo breed of India using heterologous microsatellite markers.

Abstract:

:The present study was aimed at genetic characterization and diversity analysis of Pandharpuri buffalo population using the Food and Agriculture Organization (FAO) recommended bovine microsatellite markers. Genomic DNA was isolated from blood samples of 50 unrelated animals and a total of 23 microsatellite loci were amplified using polymerase chain reaction. Among 23 recommended microsatellite markers, 14 markers (BM2113, BM1818, CSSM66, HEL13, INRA037, ILSTS05, HAUT27, INRA023, INRA035, HEL5, ETH3, NRA063, MM12 and ETH10) were found to be highly polymorphic in Pandharpuri population. The amplified products were run on polyacrylamide gel electrophoresis (PAGE) apparatus along with a ladder; subsequently, the allele typing was done based on silver staining of the gel. The effective and observed number of alleles, heterozygosity (expected and unbiased) estimates and polymorphic information content (PIC) levels were estimated for each locus. A total of 87 alleles were secured for 14 polymorphic loci studied giving an overall average of 6.21 alleles per locus. The number of alleles ranged from 2 (INRA063) to 9 (BM1818 and HEL13). The mean effective number of alleles across all polymorphic loci was found to be 4.28. The overall mean expected heterozygosity and unbiased expected heterozygosity values were 0.77 and 0.76, ranging from 0.50 (INRA063) to 0.88 (BM1818) and 0.50 (INRA063) to 0.88 (BM1818), respectively. The average PIC estimate across all polymorphic loci was 0.73 ranging from 0.373 (INRA063) to 0.864 (BM1818). In the present study, the characterization and diversity estimates are reported for Pandharpuri population. It was found to maintain optimum diversity based on 14 polymorphic microsatellite markers.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Khade KA,Panigrahi M,Ahmad SF,Kumar P,Bhushan B

doi

10.1080/10495398.2019.1612757

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

426-431

issue

5

eissn

1049-5398

issn

1532-2378

journal_volume

31

pub_type

杂志文章
  • Simple sequence repeats for genetic studies of alpaca.

    abstract::Trace sequences from the 2X alpaca genome sequencing effort were examined to identify simple sequence repeats (microsatellites) for genetic studies. A total of 6,685 repeat-containing sequences were downloaded from GenBank, processed, and assembled into contigs representing an estimated 4,278 distinct sequences. This ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802340923

    authors: Reed KM,Chaves LD

    更新日期:2008-01-01 00:00:00

  • Evaluation of Bovine High-Density SNP Genotyping Array in Indigenous Dairy Cattle Breeds.

    abstract::In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their perform...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1329150

    authors: Dash S,Singh A,Bhatia AK,Jayakumar S,Sharma A,Singh S,Ganguly I,Dixit SP

    更新日期:2018-04-03 00:00:00

  • Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing.

    abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-100108341

    authors: Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

    更新日期:2001-11-01 00:00:00

  • Genomic organization and promoter analysis of the bovine ADAM12 gene.

    abstract::A disintegrin and metalloprotease (ADAM) 12 is a member of the ADAM family possessing a putative role in a variety of biological processes such as modulation of proteolytic processing, cell adhesion, cell fusion, and signaling. Recently, it has been suggested that ADAM12 is involved in regulation of adipogenesis as we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390802212445

    authors: Taniguchi Y,Doronbekov K,Yamada T,Sasaki Y,Takano A,Sugimoto Y

    更新日期:2008-01-01 00:00:00

  • Effect of in-straw thawing on in vitro- and in vivo-development of vitrified mouse morulae.

    abstract::For the purpose of ascertaining parameters to embryo transfer on some domestic animals, mouse morulae were used as a model to investigate the effect of in-straw thawing on in vitro and in vivo-development of vitrified embryos. Embryos were vitrified in 0.25 ml straws preloaded with dilution solution (0.5 M Sucrose) an...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601091040

    authors: Yang ZQ,Zhou GB,Hou YP,Yan CL,Zhu SE

    更新日期:2007-01-01 00:00:00

  • Identification of sequence tagged sites in the Asian and African elephant.

    abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399809525902

    authors: Burk NE,Messer LA,Ernst CW,Rothschild MF

    更新日期:1998-01-01 00:00:00

  • Stage specific expression of ATP-binding cassette and solute carrier superfamily of transporter genes in mammary gland of riverine buffalo (Bubalus bubalis).

    abstract::In the present study, expression level of various ATP-binding cassette (ABC) viz., ABCA1, ABCA7, ABCG1, ABCG2, and ABCG5; associated transcription factors viz., SREBF1, LXRα (NR1H3), PPARA, and Solute Carriers (SLC); or Glucose transporters (GLUT) viz., SLC2A1(GLUT1), SLC2A4 (GLUT4), SLC2A8 (GLUT8), and SLC2A12 (GLUT1...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.839949

    authors: Sharma A,Aggarwal J,Sodhi M,Kishore A,Mishra BP,Mohanty AK,Kataria RS,Kaushik JK,Mukesh M

    更新日期:2014-01-01 00:00:00

  • SNaPshot minisequencing and a panel of candidate genes for complex routine testing of meat performance traits in pigs.

    abstract::The aim of the study was to introduce a convenient method for identification of differences among individual animals in genes supposed to influence meat performance in pigs. The set of seven candidate genes (IGF2, FOS, MC4R, DGAT1, MYF4, MYF, and MC3R) was used. To determine the genotypes, multiplex polymerase chain r...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601088657

    authors: Civánová K,Knoll A

    更新日期:2007-01-01 00:00:00

  • Next generation semiconductor based-sequencing of a nutrigenetics target gene (GPR120) and association with growth rate in Italian Large White pigs.

    abstract::The GPR120 gene (also known as FFAR4 or O3FAR1) encodes for a functional omega-3 fatty acid receptor/sensor that mediates potent insulin sensitizing effects by repressing macrophage-induced tissue inflammation. For its functional role, GPR120 could be considered a potential target gene in animal nutrigenetics. In this...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2014.881369

    authors: Fontanesi L,Bertolini F,Scotti E,Schiavo G,Colombo M,Trevisi P,Ribani A,Buttazzoni L,Russo V,Dall'Olio S

    更新日期:2015-01-01 00:00:00

  • Combined Haplotypes of CaSR Gene Sequence Variants and Their Associations with Growth Traits in Cattle.

    abstract::The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2016.1271805

    authors: Yue B,Han F,Wu J,Wang Y,Zhang C,Fang X,Qi X,Bai Y,Chen H

    更新日期:2017-10-02 00:00:00

  • A 3800 gene microarray for cattle functional genomics: comparison of gene expression in spleen, placenta, and brain.

    abstract::A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120005779

    authors: Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

    更新日期:2002-05-01 00:00:00

  • Association of genetic variability in CD209 gene with bovine paratuberculosis disease: a case-control study in the Indian cattle population.

    abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1823400

    authors: Kumar S,Kumar S,Singh RV,Chauhan A,Kumar A,Bharati J,Singh SV

    更新日期:2020-09-28 00:00:00

  • Associations of polymorphisms in four candidate genes with carcass and/or meat-quality traits in two meat-type chicken lines.

    abstract::The associations between polymorphisms of five genes, calpain 1 (CAPN1), follicle stimulating hormone beta (FSHB), follicle stimulating hormone receptor (FSHR), peroxisome proliferator-activated receptor gamma (PPARG), and retinol binding protein 7 (RBP7), and live weight, carcass composition, and meat-quality traits ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2012.742909

    authors: Sun Y,Liu R,Lu X,Hu Y,Zhao G,Zheng M,Chen J,Wang H,Wen J

    更新日期:2013-01-01 00:00:00

  • Growth, immunity, relative gene expression, carcass traits and economic efficiency of two rabbit breeds fed prebiotic supplemented diets.

    abstract::The current study designed to evaluate the effect of oligosaccharide supplemented diets on growth performance, histomorphometric changes, economic efficiency and genetic expression of some growth and immunity-relative genes. One hundred and twenty weaned male rabbits, six weeks of age of two breeds (NZW and APPRI) wer...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1800485

    authors: Abd El-Aziz AH,El-Kasrawy NI,Abd El-Hack ME,Kamel SZ,Mahrous UE,El-Deeb EM,Atta MS,Amer MS,Naiel MAE,Khafaga AF,Metwally AE,Abo Ghanima MM

    更新日期:2020-07-31 00:00:00

  • Isolation of thirty-one new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library.

    abstract::Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390009525945

    authors: Wang Z,Rohrer G,Stone R,Troyer D

    更新日期:2000-01-01 00:00:00

  • Polymorphic Microsatellite Markers for Solen grandis and Their Cross-Species Amplification in Three Other Species.

    abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2017.1367690

    authors: Nie H,Jiang L,Gao Y,Xu H,Du S,Yan X

    更新日期:2019-01-01 00:00:00

  • Mitochondrial DNA sequence analyses and phylogenetic relationships among two Nigerian goat breeds and the South African Kalahari Red.

    abstract::The first hypervariable (HV1) region of mitochondrial DNA (mtDNA) of two popular Nigerian goat breeds: West African Dwarf (WAD) (n=35) and Red Sokoto (RS) (n=37) and one exotic breed: Kalahari Red (KR) (n=38) imported from South Africa were sequenced to investigate sequence diversity, genetic structure, origin, and de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2014.977907

    authors: Awotunde EO,Bemji MN,Olowofeso O,James IJ,Ajayi OO,Adebambo AO

    更新日期:2015-01-01 00:00:00

  • Sequence characterization of S100A8 gene reveals structural differences of protein and transcriptional factor binding sites in water buffalo and yak.

    abstract::The present study was undertaken to characterize the structure of S100A8 gene and its promoter in water buffalo and yak. Sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete S100A8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. BLAST analys...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2011.581558

    authors: Kathiravan P,Goyal S,Kataria RS,Mishra BP,Jayakumar S,Joshi BK

    更新日期:2011-07-01 00:00:00

  • Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle.

    abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2011.527567

    authors: Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

    更新日期:2011-01-01 00:00:00

  • Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.

    abstract::Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2015.1102148

    authors: Yonekura S,Hirota S,Miyazaki H,Tokutake Y

    更新日期:2016-01-01 00:00:00

  • The effect of retroviral vector on uptake of human lactoferrin DNA by Yak (Bos Grunniens) spermatozoa and their fertilizability in vitro.

    abstract::The objectives of this study were to evaluate the transfection effectiveness of retroviral vector PLNCX2 in yak sperm-mediated gene transfer (SMGT) and the effect of SMGT on sperm motility and fertilizability. Human lactoferrin (hLF) DNA was ligated into PLNCX2 to construct recombinant plasmid PLNCX2-hLF, then, using ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390903196455

    authors: Zi XD,Chen SW,Liang GN,Chen DW,Zhang DW,Yin RH

    更新日期:2009-01-01 00:00:00

  • Lipopolysaccharide binding protein/CD14/ TLR4-dependent recognition of salmonella LPS induces the functional activation of chicken heterophils and up-regulation of pro-inflammatory cytokine and chemokine gene expression in these cells.

    abstract::Lipopolysaccharide (LPS) is the major pathogen-associated molecular pattern (PAMP) found in the cell wall of gram-negative bacteria and, in mammals, is recognized by the Toll-like receptor 4 (TLR4) in conjunction with the serum protein, lipopolysaccharide-binding protein (LBP), and the CD14 co-receptor. We have found ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390500264896

    authors: Kogut MH,He H,Kaiser P

    更新日期:2005-01-01 00:00:00

  • Novel polymorphism of HSP70 gene affected caprine physiological vital parameters.

    abstract::The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1726364

    authors: Mohalik PK,Sahoo SS,Mishra C,Dash SK,Nayak G

    更新日期:2020-02-12 00:00:00

  • Harvesting the genomic promise: recombineering sequences for phenotypes.

    abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120026481

    authors: Rogatcheva MB,Rund LA,Beever JE,Schook LB

    更新日期:2003-11-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-100102977

    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines.

    abstract::The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of Brucella abortus strain 544 (S544) in the spleen of mice for potency testing of live B. abortus strain 19 (S19) vaccine. IS711 and eryC gene-based qPCR were optimized for calculating copy number. The copy ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1735404

    authors: Gupta T,Rawat M,Kumar B,Varshney R,Chakravarti S,Qureshi S

    更新日期:2020-04-02 00:00:00

  • Genetic variation at the MHC in a population of introduced wild turkeys.

    abstract::Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.767267

    authors: Bauer MM,Miller MM,Briles WE,Reed KM

    更新日期:2013-01-01 00:00:00

  • Genetic structuring of nine Indian domestic goat breeds based on SNPs identified in IGF-1 gene.

    abstract::The caprine Insulin like Growth Factor1 (IGF1) gene was analyzed for identification of single nucleotide polymorphisms (SNPs) and genetic structuring of Indian goat breeds. A panel of 80 samples belonging to nine Indian goat breeds (Capra hircus) including three large sized breeds (Jamunapari, Beetal and Jakhrana); th...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2013.763815

    authors: Sharma A,Dutt G,Jayakumar S,Saroha V,Verma NK,Dixit SP

    更新日期:2013-01-01 00:00:00

  • Sequence of GDF 8 (myostatin) gene in Bubalus bubalis.

    abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390601115526

    authors: Tantia MS,Vijh RK,Mishra B,Kumar ST

    更新日期:2007-01-01 00:00:00

  • Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen.

    abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495398.2020.1863225

    authors: Ali SA,Boby N,Preena P,Singh SV,Kaur G,Ghosh SK,Nandi S,Chaudhuri P

    更新日期:2021-01-10 00:00:00