Association between polymorphisms in the SLC27A1 gene and milk production traits in Chinese Holstein cattle.

Abstract:

:We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-flanking region of the SLC27A1 gene by using polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) and DNA sequencing. We found 2 polymorphic sites: 112T>C, a synonymous mutation, in exon 3 (SNP(1)), and 64G>A in the 3'-UTR (SNP(2)). We also determined the genotypes of 330 Chinese Holstein cattle by using PCR-restriction fragment length polymorphism (RFLP). We found 3 genotypes each at SNP(1) (TT, TC, and CC) and SNP(2) (GG, GA, and AA). The association among the different genotypes at these 2 sites and milk production traits was analyzed using a least-squares procedure. The results showed that cows with genotype CC had higher milk yields than those with genotype TC (0.01 < p < 0.05). No significant difference was detected among the 3 SNP(2) genotypes in terms of milk production traits. Our results provide evidence that the C allele have potential effects on milk yield trait.

journal_name

Anim Biotechnol

journal_title

Animal biotechnology

authors

Lv Y,Wei C,Zhang L,Lu G,Liu K,Du L

doi

10.1080/10495398.2011.527567

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

1-6

issue

1

eissn

1049-5398

issn

1532-2378

pii

933388528

journal_volume

22

pub_type

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