Abstract:
:Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this study, peripheral blood samples taken at five developmental stages were used to construct 15 miRNA libraries (three biological replicates/stage): 0 days (newborn), 30 days (weaning), 60 days (weaned), and 180 and 360 days (puberty). We identified 295 known mature miRNAs. Hierarchical clustering of the miRNA expression profile showed significant differences between individuals at the neonatal and postnatal stages. Functional enrichment analysis revealed that miRNAs differentially expressed between pairwise comparisons of the developmental stages were over-represented in immune-related pathways such as toll-like receptor signaling. The time-course of expression of the over-representated miRNAs exhibited a pattern of steady decline over time, for both the complete miRNA compendium and immune-related miRNAs. We identified six marker miRNAs that were highly negatively correlated with chronologic age and enriched for genes involved in immune-related pathways. This study of a peripheral blood miRNA transcriptome offers insight into immune system development in swine and provides a resource for pig genome annotation.
journal_name
Anim Biotechnoljournal_title
Animal biotechnologyauthors
Zhong Z,Zhu X,Tang Q,Hong L,Gu Y,He Z,Tao X,Yang X,Liang Y,Shen L,Tan Y,Zeng K,Ying S,Yang Y,Lei Y,Wang Y,Gong J,Chen X,Zhou R,Zhu L,Lv Xdoi
10.1080/10495398.2020.1824920subject
Has Abstractpub_date
2021-01-18 00:00:00pages
1-10eissn
1049-5398issn
1532-2378pub_type
杂志文章abstract::The sequence of myostatin gene (growth differentiation factor 8 [GDF 8]) in Indian riverine buffalo (Bubalus bubalis) is reported. The genomic DNA as well as mRNA were sequenced. The sequence is conserved across all the livestock species. Five nonsynonymous changes as compared to cattle were found in this study and we...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390601115526
更新日期:2007-01-01 00:00:00
abstract::High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1870484
更新日期:2021-01-24 00:00:00
abstract::The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genet...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1367690
更新日期:2019-01-01 00:00:00
abstract::Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Gian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1347881
更新日期:2018-04-03 00:00:00
abstract::Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1102148
更新日期:2016-01-01 00:00:00
abstract::Interferon-a genes were cloned from six breeds of three species of two genera (three Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one European breed of Holstein cow, and two water buffalo breeds of FuAn water buffalo and FuZhong water buffalo) by direct PCR. The PCR products were di...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390500461104
更新日期:2006-01-01 00:00:00
abstract::Aim of this study was to compare different combinations of penetrating intracellular CPAs, i.e., glycerol (G), ethylene glycol (EG), propylene glycol (PG), dimethyl formamide (DM), and methyl acetamide (MA) and extracellular [egg yolk (EY), egg yolk plasma (EYP), low-density lipoproteins (LDL), and coconut water (CW)]...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2019.1698434
更新日期:2019-12-08 00:00:00
abstract::CARP, ANKRD2, and DARP belong to the ankyrin repeat protein (MARP) family and play a critical role in the integration of cytoskeletal architecture, stress response, and transcriptional regulation. In this study, we cloned the cDNA and promoter sequences of porcine ankyrin repeat protein (MARP) gene family. RT-PCR anal...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.559562
更新日期:2011-04-01 00:00:00
abstract::The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observe...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1242490
更新日期:2017-04-03 00:00:00
abstract::Local chicken populations are a major source of food in the rural areas of Algeria. However, their origin has not been well characterized. The objectives of this study were to assess genetic diversity and maternal origin of domestic chicken from five agro-ecological regions of western Algeria: coastal (CT), inland pla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1803892
更新日期:2020-08-13 00:00:00
abstract::A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1863225
更新日期:2021-01-10 00:00:00
abstract::This study aimed to explore the association of single nucleotide polymorphisms (SNPs) in CD209 gene with the occurrence of bovine paratuberculosis (PTB) disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) in Indian cattle. A total of 213 animals were preliminarily selected on the basis of physical ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1823400
更新日期:2020-09-28 00:00:00
abstract::The associations between polymorphisms of five genes, calpain 1 (CAPN1), follicle stimulating hormone beta (FSHB), follicle stimulating hormone receptor (FSHR), peroxisome proliferator-activated receptor gamma (PPARG), and retinol binding protein 7 (RBP7), and live weight, carcass composition, and meat-quality traits ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2012.742909
更新日期:2013-01-01 00:00:00
abstract::This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Surviv...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1369429
更新日期:2018-01-01 00:00:00
abstract::Meat quality traits (MQTs) are very important in the porcine industry, which are mainly determined by skeletal muscle fiber composition, extra-muscular and/or intramuscular fat content. To identify the differentially expressed candidate genes affecting the meat quality traits, first we compared the MQTs and skeletal m...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2018.1437045
更新日期:2019-01-01 00:00:00
abstract::A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120005779
更新日期:2002-05-01 00:00:00
abstract::Brucellosis is a highly contagious bacterial zoonotic infectious disease severely affecting the public health and economic features of endemic and non-endemic countries. The present study assessed the potentials of using the touch-down polymerase chain reaction (TD-PCR) compared to the conventional PCR and culture met...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1777149
更新日期:2020-06-10 00:00:00
abstract::Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structure...
journal_title:Animal biotechnology
pub_type: 杂志文章,评审
doi:10.1080/10495398.2013.870075
更新日期:2014-01-01 00:00:00
abstract::Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1198796
更新日期:2017-01-02 00:00:00
abstract::Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2016.1273835
更新日期:2017-10-02 00:00:00
abstract::Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2020.1811294
更新日期:2020-08-31 00:00:00
abstract::The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice varian...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.652325
更新日期:2012-04-01 00:00:00
abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
abstract::We assessed SLC27A1, a candidate gene for milk production traits in Chinese Holstein cattle. DNA was extracted from the blood of 48 top Chinese Holstein Cattle selected according to phenotypic character and mixed into DNA pool for SNP detection. We tested blood samples of these cattle for SNPs in exon 3 and the 3'-fla...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2011.527567
更新日期:2011-01-01 00:00:00
abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120022136
更新日期:2003-05-01 00:00:00
abstract::Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regardi...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2017.1313269
更新日期:2018-04-03 00:00:00
abstract::The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations foll...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2015.1135807
更新日期:2016-01-01 00:00:00
abstract::Fescue toxicosis (FT) reduces beef animal growth and fertility. Animals afflicted with FT typically have decreased circulating prolactin concentrations and thicker summer hair coats. Preliminary experiments examined the informativeness of a novel Dopamine Receptor 2 (DRD2) G/A SNP for resistance to FT. Steers grazed t...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2013.796960
更新日期:2014-01-01 00:00:00
abstract::Genetic variation in the major histocompatibility complex (MHC) is known to affect disease resistance in many species. Investigations of MHC diversity in populations of wild species have focused on the antigen presenting class IIβ molecules due to the known polymorphic nature of these genes and the role these molecule...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495398.2013.767267
更新日期:2013-01-01 00:00:00
abstract::Tissue-specific cDNA library sequences (expressed sequence tags, or EST) yield a detailed snapshot of gene expression and are useful in developing second-generation molecular resources (i.e., microarrays) for gene expression profiling. The objective of this study was to develop and characterize an intestine-specific c...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-200053398
更新日期:2005-01-01 00:00:00